Genome editing technologies: CRISPR, LEAPER, RESTORE, ARCUT, SATI, and RESCUE

Yılmaz, Şenay

doi:10.17179/excli2020-3070

EXCLI Journal; 20:Doc19; ISSN 1611-2156 2021

DOI: 10.17179/excli2020-3070

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Genome editing technologies: CRISPR, LEAPER, RESTORE, ARCUT, SATI, and RESCUE

Şenay Yılmaz

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Cited by 5 publications

References 141 publications

(143 reference statements)

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Optimization of CRISPR–Cas system for clinical cancer therapy

Meng

Lou

et al. 2022

Bioengineering & Transla Med

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Cancer is a genetic disease caused by alterations in genome and epigenome and is one of the leading causes for death worldwide. The exploration of disease development and therapeutic strategies at the genetic level have become the key to the treatment of cancer and other genetic diseases. The functional analysis of genes and mutations has been slow and laborious. Therefore, there is an urgent need for alternative approaches to improve the current status of cancer research. Gene editing technologies provide technical support for efficient gene disruption and modification in vivo and in vitro, in particular the use of clustered regularly interspaced short palindromic repeats (CRISPR)-Cas systems. Currently, the applications of CRISPR-Cas systems in cancer rely on different Cas effector proteins and the design of guide RNAs. Furthermore, effective vector delivery must be met for the CRISPR-Cas systems to enter human clinical trials. In this review article, we describe the mechanism of the CRISPR-Cas systems and highlight the applications of class II Cas effector proteins. We also propose a synthetic biology approach to modify the CRISPR-Cas systems, and summarize various delivery approaches facilitating the clinical application of the CRISPR-Cas systems. By modifying the CRISPR-Cas system and optimizing its

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Optimization of CRISPR–Cas system for clinical cancer therapy

Meng

Lou

et al. 2022

Bioengineering & Transla Med

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Mechanism and Applications of CRISPR/Cas-9-Mediated Genome Editing

Mengstie¹,

Zawdie²

2021

BTT

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Establishment, optimization, and application of genetic technology in Aspergillus spp.

et al. 2023

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Aspergillus is widely distributed in nature and occupies a crucial ecological niche, which has complex and diverse metabolic pathways and can produce a variety of metabolites. With the deepening of genomics exploration, more Aspergillus genomic informations have been elucidated, which not only help us understand the basic mechanism of various life activities, but also further realize the ideal functional transformation. Available genetic engineering tools include homologous recombinant systems, specific nuclease based systems, and RNA techniques, combined with transformation methods, and screening based on selective labeling. Precise editing of target genes can not only prevent and control the production of mycotoxin pollutants, but also realize the construction of economical and efficient fungal cell factories. This paper reviewed the establishment and optimization process of genome technologies, hoping to provide the theoretical basis of experiments, and summarized the recent progress and application in genetic technology, analyzes the challenges and the possibility of future development with regard to Aspergillus.

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Genome editing technologies: CRISPR, LEAPER, RESTORE, ARCUT, SATI, and RESCUE

Cited by 5 publications

References 141 publications

Optimization of CRISPR–Cas system for clinical cancer therapy

Optimization of CRISPR–Cas system for clinical cancer therapy

Mechanism and Applications of CRISPR/Cas-9-Mediated Genome Editing

Establishment, optimization, and application of genetic technology in Aspergillus spp.

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