Genome editing in rice: New paths for an old crop

Abstract

Rice is one of the most consumed cereals worldwide and feeds about 3 billion people. However, the occurrence of climate changes has influenced abiotic and biotic stresses and consequently affected the production and quality of rice grains. The development of new, higher yielding cultivars is necessary to ensure global food security. Although great progress has already been achieved by conventional breeding, biotechnological tools, such as transgenics and genome editing, can aid in meeting future demands. Gene editing is characterized by cutting and modifying target genes. Among the genome editing techniques, CRISPR/Cas (clustered regularly interspaced short palindromic repeats/Cas), has been proposed because of its ease of manipulation. Variants such as multiple Cas proteins, base editing and prime editing, which aim to increase editing efficiency have also been proposed. Edited plants are more accepted because they are transgene-free. Thus, the objective of this review is to describe the different gene editing techniques and their respective applications in rice breeding. It was observed that the CRISPR/Cas tool was efficient in gene editing in studies related to yield, tolerance to biotic and abiotic stresses, and rice grain quality. However, the impact of this approach in breeding programs depends on the cultivation of edited plants on a large scale in the field. Based on the progress made so far, it is believed that rice breeders can bet on CRISPR/Cas and its variants to help face the current and future challenges of climate change and high food demand.