Genome Editing Enables Next-Generation Hybrid Seed Production Technology

Qi, Xiantao; Zhang, Congsheng; Zhu, Jinjie; Liu, Changlin; Huang, Changling; Li, Xinhai; Xie, Chuanxiao

doi:10.1016/j.molp.2020.06.003

Cited by 47 publications

(33 citation statements)

References 26 publications

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“…Self-pollination of the transgenic plant produced 50% male sterile seeds and 50% transgenic seeds that could be sorted out based on the red fluorescence. Since then, various systems similar to SPT have been constructed in maize and rice with different NMS genes and pollen-killer genes, but all use RFP as the seed sorting marker (Chang et al 2016 ; Wu et al 2016 ; An et al 2019 ; Qi et al 2020 ; Wang et al 2020 ; Liao et al 2021 ; Song et al 2021 ).…”

Section: Resultsmentioning

confidence: 99%

Construction of a weight-based seed sorting system for the third-generation hybrid rice

Qiu

Wang

et al. 2021

Rice

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Background The third-generation hybrid rice technology can be constructed by transforming a recessive nuclear male sterile (NMS) mutant with a transgenic cassette containing three functional modules: the wild type male fertility gene to restore the fertility of the mutant, the pollen killer gene that specifically kills the pollen grains carrying the transgene, and the red fluorescence protein (RFP) gene to mark the transgenic seed (maintainer). The transgenic plant produces 1:1 NMS seeds and maintainer seeds that can be distinguished by the RFP signal. However, the RFP signals in the partially filled or pathogen-infected maintainer seeds are often too weak to be detected by RFP-based seed sorting machine, resulting in intermingling of the maintainer seeds with NMS seeds. Results Here we constructed a weight-based seed sorting system for the third-generation hybrid rice technology by silencing the genes encoding ADP-glucose pyrophosphorylase (AGP) essential for endosperm starch biosynthesis via endosperm-specific expression of artificial microRNAs (amiRNAs). In this system, the NMS seeds have normal endosperm and are heavy, but the maintainer seeds have shrunken endosperms and are light-weighted. The maintainer seeds can be easily and accurately sorted out from the NMS seeds by weight-sorting machines, so pure and fully filled NMS seeds are available. Conclusions The weight-based seed sorting system shows obvious advantages over the RFP-based seed sorting system in accuracy, efficiency, and cost for propagation of pure male sterile seeds. These characteristics will significantly increase the value and transgenic safety of the third-generation hybrid rice technology.

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Section: Resultsmentioning

confidence: 99%

Construction of a weight-based seed sorting system for the third-generation hybrid rice

Qiu

Wang

et al. 2021

Rice

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“…Therefore, these genes should be a priority to facilitate hybrid breeding. Very recently, a maize male fertility gene (ZmMS26) was targeted by the CRISPR/Cas system to produce a one-step hybrid (Qi et al, 2020). Similarly, another lipid-metabolism-related maize gene ZmMs17 has recently been used to create male sterility in rice, and interestingly, it did not affect the vegetative growth and female fertility (An et al, 2020).…”

Section: Discussionmentioning

confidence: 99%

Exploiting Genic Male Sterility in Rice: From Molecular Dissection to Breeding Applications

et al. 2021

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Rice (Oryza sativa L.) occupies a very salient and indispensable status among cereal crops, as its vast production is used to feed nearly half of the world’s population. Male sterile plants are the fundamental breeding materials needed for specific propagation in order to meet the elevated current food demands. The development of the rice varieties with desired traits has become the ultimate need of the time. Genic male sterility is a predominant system that is vastly deployed and exploited for crop improvement. Hence, the identification of new genetic elements and the cognizance of the underlying regulatory networks affecting male sterility in rice are crucial to harness heterosis and ensure global food security. Over the years, a variety of genomics studies have uncovered numerous mechanisms regulating male sterility in rice, which provided a deeper and wider understanding on the complex molecular basis of anther and pollen development. The recent advances in genomics and the emergence of multiple biotechnological methods have revolutionized the field of rice breeding. In this review, we have briefly documented the recent evolution, exploration, and exploitation of genic male sterility to the improvement of rice crop production. Furthermore, this review describes future perspectives with focus on state-of-the-art developments in the engineering of male sterility to overcome issues associated with male sterility-mediated rice breeding to address the current challenges. Finally, we provide our perspectives on diversified studies regarding the identification and characterization of genic male sterility genes, the development of new biotechnology-based male sterility systems, and their integrated applications for hybrid rice breeding.

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“…Third, our discovery of GmMS1 can greatly accelerate genetic interchange amongst different soybean cultivars. Moreover, it is even possible to envision a next‐generation hybridizing system in soybean to take full advantage of heterosis (Qi et al ., 2020). Finally, we want to emphasize that the bulking strategy we employed—based on a public control bulk comprising public resequencing data and a single mutant bulk from a recurrent population—should be suitable for identifying causal genes of other recessive male sterility loci in recurrent populations.…”

Section: Figurementioning

confidence: 99%

The cloning and CRISPR/Cas9‐mediated mutagenesis of a male sterility gene MS1 of soybean

Jiang

Chen

Yang

et al. 2021

Plant Biotechnology Journal

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Genome Editing Enables Next-Generation Hybrid Seed Production Technology

Cited by 47 publications

References 26 publications

Construction of a weight-based seed sorting system for the third-generation hybrid rice

Construction of a weight-based seed sorting system for the third-generation hybrid rice

Exploiting Genic Male Sterility in Rice: From Molecular Dissection to Breeding Applications

The cloning and CRISPR/Cas9‐mediated mutagenesis of a male sterility gene MS1 of soybean

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