Genome duplication improves the resistance of watermelon root to salt stress

Zhu, Huiling; Zhao, Shengjie; Lu, Xingrong; He, Nan; Gao, Lei; Dou, Junling; Bie, Zhilong; Liu, Wenge

doi:10.1016/j.plaphy.2018.10.019

Cited by 33 publications

(26 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Also, ploidy results in chromosome doubling leading to gene doubling, which results in higher expression, thus causing an increase in protein contents [75]. Polyploidy is more tolerant of abiotic stress than Di it was reported in watermelon [76], rice [73,77], citrus [72], black locust [78], honeysuckle [79], kinnow mandarin [80], cotton [81], and rangpur lime [82]. Peroxidase and catalase activities were increased in the grafted plants [83].…”

Section: Discussionmentioning

confidence: 95%

Comparative Physiological and Biochemical Mechanisms in Di, Tri, and Tetraploid Watermelon (<em>Citrullus lanatus</em> L.) Grafted by Branches

Kaseb¹,

Umer²,

Gebremeskel³

et al. 2020

Preprint

Self Cite

View full text Add to dashboard Cite

Polyploid seeds production is laborious, complicated, and costly work. Tetraploid and triploid plants produce a fewer number of seeds/fruit, and triploid embryos are fairly weak, covered with a more hardened seed coat as compared to diploid seeds. Here we investigated the interactive effect of new grafting technique of polyploid watermelon scion onto rootstock on plants' survival rate, biochemical, and hormones contents. In this study, three different branches, apical meristem (AM), branch with 1 node (1N), and 2 nodes (2N) from di, Tri, and tetraploid watermelon plants, were used as scion and grafted onto squash rootstock. The results showed highly significant differences between polypoid watermelon when 1N using as a scion, tetraploid showed maximum survival rates, higher contents of hormones, and antioxidants (AOX) activities, compared to diploid, these may be the possible reasons for high compatibility in tetraploid and degrading the grafting zone in diploid. RTq-PCR results confirm that the expression of genes linked to compatibility is consistent with the hormonal and AOX activities. This study provides an alternative and economical approach to produce more tetraploid and triploid plants for breeding or seeds production by using branches as scions.

show abstract

Section: Discussionmentioning

confidence: 95%

Comparative Physiological and Biochemical Mechanisms in Di, Tri, and Tetraploid Watermelon (<em>Citrullus lanatus</em> L.) Grafted by Branches

Kaseb¹,

Umer²,

Gebremeskel³

et al. 2020

Preprint

Self Cite

View full text Add to dashboard Cite

show abstract

“…The production of useful tetraploid inbred lines is essential to ensure the development of triploid hybrids with excellent yield and quality (Mason, 2017). Advantages of polyploidy such as enlarged organs (Jaskani et al , 2005; Saminathan et al , 2015), high content of secondary metabolites (Dou et al , 2017) and tolerance to different stresses including salinity, cold, flooding and Fusarium wilt have been noted in tetraploid watermelon (Zhu et al , 2018). One study (Saminathan et al , 2015) revealed that tetraploid progenitors of triploid watermelon exhibited wide phenotype differences as compared with their diploid counterparts despite no change in fruit characteristics from both lines.…”

Section: Introductionmentioning

confidence: 99%

Altered chromatin conformation and transcriptional regulation in watermelon following genome doubling

et al. 2021

View full text Add to dashboard Cite

Polyploidy has played a crucial role in plant evolution, development and function. Synthetic autopolyploid represents an ideal system to investigate the effects of polyploidization on transcriptional regulation. In this study, we deciphered the impact of genome duplication at phenotypic and molecular levels in watermelon. Overall, 88% of the genes in tetraploid watermelon followed a >1:1 dosage effect, and accordingly, differentially expressed genes were largely upregulated. In addition, a great number of hypomethylated regions (1688) were identified in an isogenic tetraploid watermelon. These differentially methylated regions were localized in promoters and intergenic regions and near transcriptional start sites of the identified upregulated genes, which enhances the importance of methylation in gene regulation. These changes were reflected in sophisticated higher-order chromatin structures. The genome doubling caused switching of 108 A and 626 B compartments that harbored genes associated with growth, development and stress responses.

show abstract

“…For example, the tetraploid watermelon contains an enlarged leaf, thicker vine and peel, larger pistil and stamen flower organs, and bigger seeds. Additionally, the tetraploid watermelon has: a higher chlorophyll content; the total β-carotene, lycopene, fructose, and glucose content is higher than diploid [4,5]; and it is more tolerant to salt stress compared to diploid [6]. Although with respect to those benefits, the low frequency of autopolyploids and the obscure observation and selection limit the application to commercial cultivation.…”

Section: Introductionmentioning

confidence: 99%

Efficient Characterization of Tetraploid Watermelon

Zhang

Bao

Xie

et al. 2019

Plants

View full text Add to dashboard Cite

Watermelon (Citrullus lanatus (Thunb.) Matsum. &Nakai) is an economic crop, which is widely cultivated around the world. The ploidy study of watermelon has an important role in field breeding and production, therefore, timely and convenient ploidy detection is necessary to accelerate its application. Traditionally, the ploidy of watermelon was determined by a series of time-consuming, expensive, and less efficient methods. In this study, we developed a more efficient method to simplify and accelerate the polyploidy identification in watermelons. We first confirmed the ploidy of watermelon by traditional tetraploid morphological features and well-established flow cytometry (FCM). Then we developed a reliable real-time quantitative PCR (qPCR) technique by quantifying the highly conserved 5S rDNA sequence and its copy numbers. This technique requires less sample collection and has comparable accuracy to FCM, it accelerates the analysis process and provides a new method for the identification of polyploidy of watermelon.

show abstract

Genome duplication improves the resistance of watermelon root to salt stress

Cited by 33 publications

References 34 publications

Comparative Physiological and Biochemical Mechanisms in Di, Tri, and Tetraploid Watermelon (<em>Citrullus lanatus</em> L.) Grafted by Branches

Comparative Physiological and Biochemical Mechanisms in Di, Tri, and Tetraploid Watermelon (<em>Citrullus lanatus</em> L.) Grafted by Branches

Altered chromatin conformation and transcriptional regulation in watermelon following genome doubling

Efficient Characterization of Tetraploid Watermelon

Contact Info

Product

Resources

About