Genome Defense

Clark, David P.; Pazdernik, Nanette J.; McGehee, Michelle R.

doi:10.1016/b978-0-12-813288-3.00020-3

Cited by 4 publications

(8 citation statements)

References 8 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Both classes of CRISPR systems have a significant potential for genome editing; however effector complexes of class 2 systems are more simplified and, thus, they are preferable in genetic engineering. Genome editing via CRISPR class 2 systems utilizes an artificial single-guide RNA (sgRNA) that consists of tracrRNA, crRNA, and an artificial RNA linker [ 21 ]. In contrast to artificial sgRNAs, a natural guide RNA (gRNA) in type II systems, also termed a crRNA–tracrRNA complex consists only of tracrRNA and crRNA without the linker ( Figure 6 A).…”

Section: Applications Of Crispr–cas Systemsmentioning

confidence: 99%

“…Further modifications were carried out on the Cas9 active sites. These modifications in the CRISPR–Cas9 system improved some mechanisms compared to the wild-type system, e.g., in DNA targeting or the introduction of single-stranded breaks (SSB) in DNA instead of double-stranded breaks (DSB) [ 21 ].…”

Section: Applications Of Crispr–cas Systemsmentioning

confidence: 99%

“…HDR allows for inserting novel genes (knock-in) or “knockout” existing genes. The homologous sequences can be provided exogenously and utilized to target genome editing [ 21 , 28 ].…”

Section: Applications Of Crispr–cas Systemsmentioning

confidence: 99%

“…The CBEs convert a C–G base pair into a T–A pair, whereas ABEs convert the A–T pair into a G–C base pair. Both base editors thus can provide all four possible conversions (C→T, A→G, T→C, and G→A) [ 21 , 29 ].…”

Section: Applications Of Crispr–cas Systemsmentioning

confidence: 99%

See 3 more Smart Citations

Novel CRISPR–Cas Systems: An Updated Review of the Current Achievements, Applications, and Future Research Perspectives

Nidhi

Anand

Olekšák

et al. 2021

IJMS

144

View full text Add to dashboard Cite

According to Darwin’s theory, endless evolution leads to a revolution. One such example is the Clustered Regularly Interspaced Palindromic Repeats (CRISPR)–Cas system, an adaptive immunity system in most archaea and many bacteria. Gene editing technology possesses a crucial potential to dramatically impact miscellaneous areas of life, and CRISPR–Cas represents the most suitable strategy. The system has ignited a revolution in the field of genetic engineering. The ease, precision, affordability of this system is akin to a Midas touch for researchers editing genomes. Undoubtedly, the applications of this system are endless. The CRISPR–Cas system is extensively employed in the treatment of infectious and genetic diseases, in metabolic disorders, in curing cancer, in developing sustainable methods for fuel production and chemicals, in improving the quality and quantity of food crops, and thus in catering to global food demands. Future applications of CRISPR–Cas will provide benefits for everyone and will save countless lives. The technology is evolving rapidly; therefore, an overview of continuous improvement is important. In this review, we aim to elucidate the current state of the CRISPR–Cas revolution in a tailor-made format from its discovery to exciting breakthroughs at the application level and further upcoming trends related to opportunities and challenges including ethical concerns.

show abstract

Section: Applications Of Crispr–cas Systemsmentioning

confidence: 99%

Section: Applications Of Crispr–cas Systemsmentioning

confidence: 99%

“…HDR allows for inserting novel genes (knock-in) or “knockout” existing genes. The homologous sequences can be provided exogenously and utilized to target genome editing [ 21 , 28 ].…”

Section: Applications Of Crispr–cas Systemsmentioning

confidence: 99%

Section: Applications Of Crispr–cas Systemsmentioning

confidence: 99%

See 2 more Smart Citations

Novel CRISPR–Cas Systems: An Updated Review of the Current Achievements, Applications, and Future Research Perspectives

Nidhi

Anand

Olekšák

et al. 2021

IJMS

144

View full text Add to dashboard Cite

show abstract

“…CRISPR is considered the third generation of gene editing tools, and it has been developed from a natural defense mechanism that helps bacteria fight viral invasion. Further, the CRISPR system is divided into two classes and six types, with more than 19 subtypes and many associated proteins [10]. There are many CRISPR platforms that have been developed in order to combat COVID-19, such as SHERLOCK and DETECTR [7].…”

mentioning

confidence: 99%

CRISPR as a novel technique for COVID-19 diagnosis: a review

Saleem¹,

Annooz²,

Hadi³

et al. 2023

Ukr.Biochem.J.

View full text Add to dashboard Cite

To this moment, the human coronavirus disease COVID-19 that occurs as a result of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) infection is still a critical case that provokes concern around the world. In January 2022, there were over 300 million infections and over 5 million fatalities from COVID-19. As a countermeasure against this rapid spread, there is a vital need for effective and low-cost diagnosis methods in order to control the danger of this pandemic. CRISPR technology has proved its efficiency in detecting COVID-19 due to its simplicity, specificity and high sensitivity. This paper reviews the state-of-the-art of developing the CRISPR platforms for the purpose of COVID-19 diagnosis and treatment. Limitations and challenges of CRISPR in terms of nucleic acid analytical methods for viral infection diagnosis are discussed. Keywords: Cas protein, COVID-19, CRISPR, RT-PCR, SARS-COV-2

show abstract

In Silico Evidence of the Multifunctional Features of Lactiplantibacillus pentosus LPG1, a Natural Fermenting Agent Isolated from Table Olive Biofilms

López-García

Benítez-Cabello

Ramiro-García

et al. 2023

Foods

View full text Add to dashboard Cite

In recent years, there has been a growing interest in obtaining probiotic bacteria from plant origins. This is the case of Lactiplantibacillus pentosus LPG1, a lactic acid bacterial strain isolated from table olive biofilms with proven multifunctional features. In this work, we have sequenced and closed the complete genome of L. pentosus LPG1 using both Illumina and PacBio technologies. Our intention is to carry out a comprehensive bioinformatics analysis and whole-genome annotation for a further complete evaluation of the safety and functionality of this microorganism. The chromosomic genome had a size of 3,619,252 bp, with a GC (Guanine-Citosine) content of 46.34%. L. pentosus LPG1 also had two plasmids, designated as pl1LPG1 and pl2LPG1, with lengths of 72,578 and 8713 bp (base pair), respectively. Genome annotation revealed that the sequenced genome consisted of 3345 coding genes and 89 non-coding sequences (73 tRNA and 16 rRNA genes). Taxonomy was confirmed by Average Nucleotide Identity analysis, which grouped L. pentosus LPG1 with other sequenced L. pentosus genomes. Moreover, the pan-genome analysis showed that L. pentosus LPG1 was closely related to the L. pentosus strains IG8, IG9, IG11, and IG12, all of which were isolated from table olive biofilms. Resistome analysis reported the absence of antibiotic resistance genes, whilst PathogenFinder tool classified the strain as a non-human pathogen. Finally, in silico analysis of L. pentosus LPG1 showed that many of its previously reported technological and probiotic phenotypes corresponded with the presence of functional genes. In light of these results, we can conclude that L. pentosus LPG1 is a safe microorganism and a potential human probiotic with a plant origin and application as a starter culture for vegetable fermentations.

show abstract

Genome Defense

Cited by 4 publications

References 8 publications

Novel CRISPR–Cas Systems: An Updated Review of the Current Achievements, Applications, and Future Research Perspectives

Novel CRISPR–Cas Systems: An Updated Review of the Current Achievements, Applications, and Future Research Perspectives

CRISPR as a novel technique for COVID-19 diagnosis: a review

In Silico Evidence of the Multifunctional Features of Lactiplantibacillus pentosus LPG1, a Natural Fermenting Agent Isolated from Table Olive Biofilms

Contact Info

Product

Resources

About