Genome Comparison of Erythromycin Resistant Campylobacter from Turkeys Identifies Hosts and Pathways for Horizontal Spread of erm(B) Genes

Florez‐Cuadrado, Diego; Ugarte‐Ruiz, María; Méric, Guillaume; Quesada, Alberto; Porrero, M. Concepción; Pascoe, Ben; Sáez-Llorente, José Luis; Orozco, Gema López; Domı́nguez, Lucas; Sheppard, Samuel K.

doi:10.3389/fmicb.2017.02240

Cited by 38 publications

(43 citation statements)

References 45 publications

(70 reference statements)

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“…). Furthermore, a C. jejuni plasmid (pCJ14980A; Accession: CP017030.1) previously isolated from turkey faeces (Florez‐Cuadrado et al ., ) was identified in a C. jejuni isolate from cattle in our study (Fig. ).…”

Section: Resultssupporting

confidence: 54%

“…In addition to tetO , our analyses identified 14 other accessory genes associated with Campylobacter resistance to other known antimicrobial classes (Supporting Information Table S4). These included aminoglycosides (10 genes), β‐lactams ( bla OXA‐61 ) and macrolides ( erm B) that have been variously used as treatments targeting Campylobacter and other infectious agents (or even as growth promoters; Engberg et al ., ) in humans and animals (Lambert et al ., ; Engberg et al ., ; Griggs et al ., ; Qin et al ., , ; Chen et al ., ; Toth et al ., ; Florez‐Cuadrado et al ., , ; Lapierre et al ., ; Yao et al ., ). Initial evidence of the importance of HGT in the transmission of these genes can be seen with inconsistent topology of individual AMR gene trees, compared to the Campylobacter core genome phylogeny (Supporting Information Fig.…”

Section: Discussionmentioning

confidence: 99%

“…Fifty microliters of this suspension was added to 11 ml of Mueller-Hinton broth (TREK Diagnostics Systems, Waltham, MA) supplemented with 5.5% lysed horse blood (Oxoid). The solution was poured onto EUCAMP2 microdilution plates (TREK Diagnostics Systems) which were incubated under microaerophilic conditions for 48 h at 37 C as previously described (Florez-Cuadrado et al, 2017). The interpretation of the quantitative data was performed according to the European Committee of Antimicrobial Susceptibility Testing, EUCAST (http:// www.eucast.org/; last accessed: 06/2017).…”

Section: Culture and Antimicrobial Susceptibility Testingmentioning

confidence: 99%

“…For genome sequencing, isolates stored at −80 C in 1% protease peptone and 10% glycerol broth were cultured onto blood agar plates (bioMérieux) in microaerophilic conditions at 42 C for 48 h as previously described (Florez-Cuadrado et al, 2017). Genomic DNA was extracted using the QIAamp DNA Mini Kit (QIAGEN, Crawley, U.K.), according to manufacturer's instructions.…”

Section: Dna Extraction Genome Sequencing and Archivingmentioning

confidence: 99%

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Gene pool transmission of multidrug resistance among Campylobacter from livestock, sewage and human disease

Mourkas

Florez‐Cuadrado

Pascoe

et al. 2019

Environmental Microbiology

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Summary The use of antimicrobials in human and veterinary medicine has coincided with a rise in antimicrobial resistance (AMR) in the food‐borne pathogens Campylobacter jejuni and Campylobacter coli. Faecal contamination from the main reservoir hosts (livestock, especially poultry) is the principal route of human infection but little is known about the spread of AMR among source and sink populations. In particular, questions remain about how Campylobacter resistomes interact between species and hosts, and the potential role of sewage as a conduit for the spread of AMR. Here, we investigate the genomic variation associated with AMR in 168 C. jejuni and 92 C. coli strains isolated from humans, livestock and urban effluents in Spain. AMR was tested in vitro and isolate genomes were sequenced and screened for putative AMR genes and alleles. Genes associated with resistance to multiple drug classes were observed in both species and were commonly present in multidrug‐resistant genomic islands (GIs), often located on plasmids or mobile elements. In many cases, these loci had alleles that were shared among C. jejuni and C. coli consistent with horizontal transfer. Our results suggest that specific antibiotic resistance genes have spread among Campylobacter isolated from humans, animals and the environment.

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Section: Resultssupporting

confidence: 54%

Section: Discussionmentioning

confidence: 99%

Section: Culture and Antimicrobial Susceptibility Testingmentioning

confidence: 99%

Section: Dna Extraction Genome Sequencing and Archivingmentioning

confidence: 99%

See 2 more Smart Citations

Gene pool transmission of multidrug resistance among Campylobacter from livestock, sewage and human disease

Mourkas

Florez‐Cuadrado

Pascoe

et al. 2019

Environmental Microbiology

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“…It is noteworthy that, since the first report of erm(B) in Campylobacter (14), novel genetic environments have continuously been described. Analysis of all published studies of erm(B) in Campylobacter (14,15,17,18,(22)(23)(24)(25) shows that type VI and type III are the predominant MDRGI types in this genus, with both types consistently being represented in isolates from humans and animals. This finding suggests that these two MDRGI types are widely disseminated among Campylobacter isolates (Table 2).…”

Section: Characterization Of Erm(b)-carrying C Jejuni Isolatesmentioning

confidence: 99%

Emerging erm (B)-Mediated Macrolide Resistance Associated with Novel Multidrug Resistance Genomic Islands in Campylobacter

Liu

et al. 2019

Antimicrob Agents Chemother

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The rapid dissemination of the macrolide resistance gene erm(B) will likely compromise the efficacy of macrolides as the treatment of choice for campylobacteriosis. More importantly, erm(B) is always associated with several multidrug resistance genomic islands (MDRGIs), which confer resistance to multiple other antimicrobials. Continuous monitoring of the emergence of erm(B) and analysis of its associated genetic environments are crucial for our understanding of macrolide resistance in Campylobacter. In this study, 290 Campylobacter isolates (216 Campylobacter coli isolates and 74 Campylobacter jejuni isolates) were obtained from 1,039 fecal samples collected in 2016 from pigs and chickens from three regions of China (344 samples from Guangdong, 335 samples from Shanghai, and 360 samples from Shandong). Overall, 74 isolates (72 C. coli isolates and 2 C. jejuni isolates) were PCR positive for erm (B). Combined with data from previous years, we observed a trend of increasing prevalence of erm(B) in C. coli. Pulsed-field gel electrophoresis analyses suggested that both clonal expansion and horizontal transmission were involved in the dissemination of erm(B) in C. coli, and three novel types of erm(B)-associated MDRGIs were identified among the isolates. Furthermore, 2 erm(B)-harboring C. jejuni isolates also contained an aminoglycoside resistance genomic island and a multidrug-resistance-enhancing efflux pump, encoded by RE-cmeABC. Antimicrobial susceptibility testing showed that most of the isolates were resistant to all clinically important antimicrobial agents used for the treatment of campylobacteriosis. These findings suggest that the increasing prevalence of erm(B)-associated MDRGIs might further limit treatment options for campylobacteriosis.

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Natural Competence and Horizontal Gene Transfer in Campylobacter

Golz

Stingl

2021

Current Topics in Microbiology and Immunology

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Thermophilic Campylobacter, in particular Campylobacter jejuni, C. coli and C. lari are the main relevant Campylobacter species for human infections. Due to their high capacity of genetic exchange by horizontal gene transfer (HGT), rapid adaptation to changing environmental and host conditions contribute to successful spreading and persistence of these foodborne pathogens. However, extensive HGT can exert dangerous side effects for the bacterium, such as the incorporation of gene fragments leading to disturbed gene functions. Here we discuss mechanisms of HGT, notably natural transformation, conjugation and bacteriophage transduction and limiting regulatory strategies of gene transfer. In particular, we summarize the current knowledge on how the DNA macromolecule is exchanged between single cells. Mechanisms to stimulate and to limit HGT obviously coevolved and maintained an optimal balance. Chromosomal rearrangements and incorporation of harmful mutations are risk factors for survival and can result in drastic loss of fitness. In Campylobacter, the restricted recognition and preferential uptake of free DNA from relatives are mediated by a short methylated DNA pattern and not by a classical DNA uptake sequence as found in other bacteria. A class two CRISPR-Cas system is present but also other DNases and restriction–modification systems appear to be important for Campylobacter genome integrity. Several lytic and integrated bacteriophages have been identified, which contribute to genome diversity. Furthermore, we focus on the impact of gene transfer on the spread of antibiotic resistance genes (resistome) and persistence factors. We discuss remaining open questions in the HGT field, supposed to be answered in the future by current technologies like whole-genome sequencing and single-cell approaches.

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Genome Comparison of Erythromycin Resistant Campylobacter from Turkeys Identifies Hosts and Pathways for Horizontal Spread of erm(B) Genes

Cited by 38 publications

References 45 publications

Gene pool transmission of multidrug resistance among Campylobacter from livestock, sewage and human disease

Gene pool transmission of multidrug resistance among Campylobacter from livestock, sewage and human disease

Emerging erm (B)-Mediated Macrolide Resistance Associated with Novel Multidrug Resistance Genomic Islands in Campylobacter

Natural Competence and Horizontal Gene Transfer in Campylobacter

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