Genome characterization and taxonomy of Plantago asiatica mosaic potexvirus

Solovyev, Andrey G.; Novikov, V. K.; Merits, Andres; Savenkov, Eugene I.; Zelenina, D. A.; Tyulkina, L.G.; Morozov, Sergey Y.

doi:10.1099/0022-1317-75-2-259

Cited by 42 publications

(19 citation statements)

References 39 publications

(42 reference statements)

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“…Uncapped FoMV synthetic transcripts were not infectious. Potexvirus genomes have a 5 -cap structure and the use of capped transcripts has been found essential for infectivity with other viruses as well [6,15,16,28,42]. The percentage of transcripts that are actually capped during in vitro synthesis was not determined, but it is estimated to be about 67% by the Ambion kit used to generate the transcripts.…”

Section: Discussionmentioning

confidence: 99%

“…Several counterparts to ORF 5A have been reported for other potexviruses including PMV [2,39], CYMV [1,40,46], SMEaV [17], and Plantago asiatica mosaic virus [42] which encode 5, 45, 19, and 14 amino acids on the N-terminus of the coat protein, respectively. However, in vivo synthesis of extended CP products have not been reported for any of these potexviruses.…”

Section: Discussionmentioning

confidence: 99%

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The open reading frame 5A of foxtail mosaic virus is expressed in vivo and is dispensable for systemic infection

Robertson

French

Morris

2000

Archives of Virology

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Summary. Infectious transcripts were successfully derived from full-length cDNA clones of foxtail mosaic potexvirus (FoMV). Full-length clones were constructed by RT-PCR whereby 5 and 3 genomic segments of 2.7 and 3.4 kb, respectively, were ligated into Bluescript II KS. The in vitro RNA transcripts were infectious to moncotyledonous (barley) and dicotyledonous (Chenopodium amaranticolor) plant species. Individual mutation studies on clones of each of the five major ORFs confirmed predicted gene function for the polymerase, TGB (triple gene block), and coat protein (CP) genes. Protoplast studies on expression of a unique open reading frame, ORF 5A, which initiates 143 nts upstream of the CP before it "reads through" the CP, revealed that the 5A protein was produced in vivo. Mutation analysis of the 5A ORF indicated, however, that it was not required for either replication or for productive infection of plants. However, the nucleic acid sequences encoding the extended CP segment were shown to be important for CP expression. Additional mutations in 5A had no effect on FoMV replication in protoplasts but rendered the virus noninfectious to plants. A correlation with diminished CP production from both mutant clones implies that synthesis of subgenomic CP mRNA was compromised, and this limited systemic infection.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

The open reading frame 5A of foxtail mosaic virus is expressed in vivo and is dispensable for systemic infection

Robertson

French

Morris

2000

Archives of Virology

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“…To date, however, neither the viral factors nor the mechanisms underlying these variations have been elucidated. Plantago asiatica mosaic virus (PlAMV) is a member of the genus Potexvirus which has a relatively broad host range including the monocotyledon lily, dicotyledon Plantago asiatica L., and the woody plant Nandina domestica (Komatsu et al, 2008;Kostin & Volkov, 1976;Solovyev et al, 1994;Hughes et al, 2005). We previously determined the complete genomic sequence of PlAMV lily isolates (Komatsu et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

Variability in the level of RNA silencing suppression caused by triple gene block protein 1 (TGBp1) from various potexviruses during infection

Senshu

Ozeki

Komatsu

et al. 2009

Journal of General Virology

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RNA silencing is an important defence mechanism against virus infection, and many plant viruses encode RNA silencing suppressors as a counter defence. In this study, we analysed the RNA silencing suppression ability of multiple virus species of the genus Potexvirus. Nicotiana benthamiana plants exhibiting RNA silencing of a green fluorescent protein (GFP) transgene showed reversal of GFP fluorescence when systemically infected with potexviruses. However, the degree of GFP fluorescence varied among potexviruses. Agrobacterium-mediated transient expression assay in N. benthamiana leaves demonstrated that the triple gene block protein 1 (TGBp1) encoded by these potexviruses has drastically different levels of silencing suppressor activity, and these differences were directly related to variations in the silencing suppression ability during virus infection. These results suggest that suppressor activities differ even among homologous proteins encoded by viruses of the same genus, and that TGBp1 contributes to the variation in the level of RNA silencing suppression by potexviruses. Moreover, we investigated the effect of TGBp1 encoded by Plantago asiatica mosaic virus (PlAMV), which exhibited a strong suppressor activity, on the accumulation of microRNA, virus genomic RNA and virus-derived small interfering RNAs. INTRODUCTIONRNA silencing is an RNA-guided gene regulatory mechanism that operates in a wide variety of eukaryotic organisms. RNA silencing begins with processing of an RNA trigger into small RNAs (~21-30 nt) by the RNase III-type enzyme Dicer (Bernstein et al., 2001). In plants, small RNAs are divided into two classes: small interfering RNAs (siRNAs) and microRNAs (miRNAs) (Mallory & Vaucheret, 2006;Brodersen & Voinnet, 2006). siRNAs are generated from double-stranded RNAs (dsRNAs) which are usually derived from transgenes, viruses or endogenous non-coding RNA genes. On the other hand, miRNAs are generated from genome-encoded precursor RNAs with imperfect stem-loop structures. These small RNAs are then incorporated into multicomponent RNA-induced silencing complexes (RISC), which contain an Argonaute (AGO) family protein and lead to homologous RNA cleavage or translational repression, and (or) DNA/chromatin methylation (Hammond, 2005;Xie et al., 2004;Chapman & Carrington, 2007).One of the important roles of RNA silencing in plants is antiviral defence (Ratcliff et al., 1997;Vance & Vaucheret, 2001;Baulcombe, 2004;Ding & Voinnet, 2007). During virus infection, dsRNAs derived from viral replication intermediates or highly structured viral genomic RNAs trigger RNA silencing directed against the viruses. Consequently, initially symptomatic plants recover from infection and become resistant to secondary infection with homologous viruses. To counteract RNA silencing, viruses have evolved RNA silencing suppressors. More than 40 RNA silencing suppressors have been identified in plant, animal, insect and fungal viruses (Li & Ding, 2006). These suppressors do not have obvious sequence similarity to one another, and t...

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“…Plantago asiatica mosaic virus (PlAMV; genus Potexvirus, family Alphaflexiviridae, order Tymovirales) was initially identified from P. asiatica, a member of the family Plantaginaceae, and the complete genomic sequence of PlAMV was determined about two decades later [10]. To date, the complete genomic sequences of 17 PlAMV isolates are available in the GenBank database.…”

mentioning

confidence: 99%

“…To date, the complete genomic sequences of 17 PlAMV isolates are available in the GenBank database. Although the first-reported isolate was characterized from P. asiatica [10], the other isolates of PlAMV have been identified in Lilium spp., Nandina domestica, and Primula sieboldii [3,5] except for one PlAMV isolate from P. asiatica in this work. The majority of these have been identified in Lilium spp.…”

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confidence: 99%

Molecular detection and characterization of a divergent isolate of Plantago asiatica mosaic virus in Plantago asiatica

et al. 2016

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Four samples of Plantago asiatica showing mottle or mottled yellowing symptoms were collected and three of them were confirmed to be infected with Plantago asiatica mosaic virus (PlAMV) by Illumina HiSeq 2500 paired-end RNA sequencing. Consequently, the complete genomic sequence of a Korean isolate of PlAMV (PlAMV isolate Gunwi) from P. asiatica was determined. The nucleotide sequence of PlAMV-Gunwi was most closely related to a Russian isolate of PlAMV (PlAMV-Ru) collected from P. asiatica but highly dissimilar (*23 %) to other isolates of PlAMV from other plant species. Pairwise comparisons revealed that the complete replicase protein and coat protein of PlAMV-Gunwi share 85.15-91.20 and 84.54-90.82 % amino acid sequence identities, respectively, to the corresponding proteins of other PlAMV isolates. To our knowledge, this is the first report of the natural infection of P. asiatica by PlAMV in Korea, and the divergent PlAMV isolate Gunwi expands our understanding of the epidemiology of PlAMV.

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Genome characterization and taxonomy of Plantago asiatica mosaic potexvirus

Cited by 42 publications

References 39 publications

The open reading frame 5A of foxtail mosaic virus is expressed in vivo and is dispensable for systemic infection

The open reading frame 5A of foxtail mosaic virus is expressed in vivo and is dispensable for systemic infection

Variability in the level of RNA silencing suppression caused by triple gene block protein 1 (TGBp1) from various potexviruses during infection

Molecular detection and characterization of a divergent isolate of Plantago asiatica mosaic virus in Plantago asiatica

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