Genome Characteristics Reveal the Biocontrol Potential of Actinobacteria Isolated From Sugarcane Rhizosphere

Wang, Zhen; Solanki, Manoj Kumar; Yu, Zhuo‐Xin; Anas, Muhammad; Dong, Dan; Xing, Yong‐Xiu; Malviya, Mukesh Kumar; Pang, Fei; Li, Yang–Rui

doi:10.3389/fmicb.2021.797889

Cited by 26 publications

(20 citation statements)

References 118 publications

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“…miltiorrhiza were obtained from the root surface. The isolation of actinomycetes was performed according to the methods described previously with modifications ( Wang et al, 2021 ). Briefly, 10 ml of soil suspension containing 1 g rhizosphere soil and 10 ml sterile water was incubated in a shaker at 100 rpm for 30 min, then diluted into 10 −3 g/ml, 10 −4 g/ml, and 10 −5 g/ml.…”

Section: Methodsmentioning

confidence: 99%

Biological control and plant growth promotion properties of Streptomyces albidoflavus St-220 isolated from Salvia miltiorrhiza rhizosphere

Wang

Jiang³

et al. 2022

Front. Plant Sci.

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Root rot disease caused by Fusarium oxysporum is a devastating disease of Salvia miltiorrhiza and dramatically affected the production and quality of Sa. miltiorrhiza. Besides the agricultural and chemical control, biocontrol agents can be utilized as an additional solution. In the present study, an actinomycete that highly inhibited F. oxysporum was isolated from rhizosphere soil and identified as based on morphological and molecular characteristics. Greenhouse assay proved that the strain had significant biological control effect against Sa. miltiorrhiza root rot disease and growth-promoting properties on Sa. miltiorrhiza seedlings. To elucidate the biocontrol and plant growth-promoting properties of St-220, we employed an analysis combining genome mining and metabolites detection. Our analyses based on genome sequence and bioassays revealed that the inhibitory activity of St-220 against F. oxysporum was associated with the production of enzymes targeting fungal cell wall and metabolites with antifungal activities. Strain St-220 possesses phosphate solubilization activity, nitrogen fixation activity, siderophore and indole-3-acetic acid production activity in vitro, which may promote the growth of Sa. miltiorrhiza seedlings. These results suggest that St. albidoflavus St-220 is a promising biocontrol agent and also a biofertilizer that could be used in the production of Sa. miltiorrhiza.

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Section: Methodsmentioning

confidence: 99%

Biological control and plant growth promotion properties of Streptomyces albidoflavus St-220 isolated from Salvia miltiorrhiza rhizosphere

Wang

Jiang³

et al. 2022

Front. Plant Sci.

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“…When choosing biomass, the main factor should be their availability and cheapness. The original biomass can be obtained from industrial waste, for example, biotechnological industries, from the natural environment, or easily grown under certain conditions [16][17][18][19].…”

Section: Introductionmentioning

confidence: 99%

“…Thus, it is Karavaiko G.I. et al [17] found that the choice for biosorption is a serious problem for the development of cheaper and more efficient technologies. Studies on the biosorption of metals by actinomycetes are relatively few.…”

Section: Introductionmentioning

confidence: 99%

The characteristics of actinomyces strptomyces griseorubiginosus 83 – the effective biosorbent of silver

Bekmukhamedova

Khamidova²

2023

E3S Web Conf.

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The bacteria bound about the same amount of silver at the time of inoculation and when added after 24 hours Fourier transform infrared spectroscopy (FT-IR) and measurements of the ζ- potential showed that several groups, mainly carboxy groups are involved in the binding and biotransformation of silver cations. Also, volatile organic compounds profiling for L. lactis, demonstrated the presence of many compounds in the free space of bacteria, which were typical products of bacterial metabolism. Finally, it can be stated that the combination of various instrumental methods used has opened up new possibilities for the interpretation of physicochemical phenomena occurring at the interface between bacteria-bacteria and bacteria-metals- bacteria. The ability of the biomass of cultures of various taxonomic groups to sorb metals is used both for purification of wastewater from metals and in the additional separation of valuable and environmentally significant metals from various wastes. In this work 48 strains of actinomycetes were isolated from various samples of desert soils of Uzbekistan under agricultural crops. As a result of screening, a strain of Streptomyces sp. 83, efficiently absorbs silver from solutions. The morphological-cultural and physiological-biochemical characteristics of the selected

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“…vasinfectum , the pathogen of fusarium wilt in cotton [ 15 ]. Streptomyces griseorubiginosus regulated plant defense enzyme activities and induced sugarcane smut resistance by regulating stress-related enzyme activities in sugarcane [ 16 ]. Streptomyces sichuanensis produced siderophore and antifungal metabolites that work against F. oxysporum f.sp.…”

Section: Introductionmentioning

confidence: 99%

A Rhizobacterium, Streptomyces albulus Z1-04-02, Displays Antifungal Activity against Sclerotium Rot in Mungbean

Ruangwong

Kunasakdakul

Chankaew

et al. 2022

Plants

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Sclerotium rot causes damping-off and stem rot in seedlings and mature mungbeans, which negatively impacts cultivation. The use of a rhizobacterium to control soil-borne diseases is an alternative method to the excess use of synthetic fungicides; therefore, this study aims to screen rhizosphere actinobacteria with fungicidal activities against Sclerotium rolfsii, the pathogen that causes sclerotium rot in mungbeans. Primary screening showed that the Streptomyces sp. isolate Z1-04-02 displayed the highest effectiveness against S. rolfsii in dual culture plates, with a percentage inhibition of 74.28%. An assay containing enzymes that degrade cell walls, of the cell-free culture filtrate (CF) of Z1-04-02, showed that the activities of chitinase and β-1,3-glucanase were 0.0209 and 1.0210 U/mL, respectively, which was significantly higher than that of the control (media alone). The cell-free CF of Z1-04-02, incubated at 37 °C and 100 °C, using agar well diffusion, effectively inhibited the growth of S. rolfsii with inhibition percentages of 37.78% and 27.78%, respectively. Solid-phase microextraction (SPME) was applied to trap volatiles released from Z1-04-02 and gas chromatography–mass spectrometry (GC/MS); volatile antifungal compounds were tentatively identified as bicyclic monoterpene (1R)-(-)-myrtenal. The application of the cell-free CF, and the spore suspension of Z1-04-02, showed disease severity indexes (DSIs) of 12.5% and 8.25%, respectively, which were significantly lower than those showing inoculation by S. rolfsii alone. The identification of this strain by morphology, biochemistry tests, and 16s rDNA sequences revealed that Z1-04-02 was Streptomyces albulus. This finding revealed that S. albulus Z1-04-02 displayed diverse fungicidal activities against S. rolfsii, and it has the potential to act as a biological control agent in terms of inhibiting sclerotium rot in mungbeans.

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Genome Characteristics Reveal the Biocontrol Potential of Actinobacteria Isolated From Sugarcane Rhizosphere

Cited by 26 publications

References 118 publications

Biological control and plant growth promotion properties of Streptomyces albidoflavus St-220 isolated from Salvia miltiorrhiza rhizosphere

Biological control and plant growth promotion properties of Streptomyces albidoflavus St-220 isolated from Salvia miltiorrhiza rhizosphere

The characteristics of actinomyces strptomyces griseorubiginosus 83 – the effective biosorbent of silver

A Rhizobacterium, Streptomyces albulus Z1-04-02, Displays Antifungal Activity against Sclerotium Rot in Mungbean

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