Genome Assembly of Serratia marcescens Type Strain ATCC 13880

Daligault, Hajnalka E.; Davenport, Karen W.; Minogue, Timothy D.; Broomall, S. M.; Bruce, David; Chain, Patrick; Coyne, S. R.; Gibbons, Henry S.; Jaissle, J.; Rosenzweig, C. Nicole; Scholz, Matthew; Teshima, Hazuki; Johnson, Shannon L.

doi:10.1128/genomea.00967-14

Cited by 10 publications

(9 citation statements)

References 8 publications

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“…marcescens Bizio (ATCC 13880) was obtained from the American Type Culture Collection and was the primary strain used in this study. The genome sequence of this strain has been recently published [ 29 ]. The non-pigmented, Drosophila isolate Db10 [ 30 , 31 ] was a gift from the lab of Dr. Jonathan J. Ewbank (Centre d’Immunologie de Marseille-Luminy, Université de la Méditerranée, Marseille, France).…”

Section: Methodsmentioning

confidence: 99%

Carbon-Starvation Induces Cross-Resistance to Thermal, Acid, and Oxidative Stress in Serratia marcescens

2015

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The broad host-range pathogen Serratia marcescens survives in diverse host and non-host environments, often enduring conditions in which the concentration of essential nutrients is growth-limiting. In such environments, carbon and energy source starvation (carbon-starvation) is one of the most common forms of stress encountered by S. marcescens. Related members of the family Enterobacteriaceae are known to undergo substantial changes in gene expression and physiology in response to the specific stress of carbon-starvation, enabling non-spore-forming cells to survive periods of prolonged starvation and exposure to other forms of stress (i.e., starvation-induced cross-resistance). To determine if carbon-starvation also results in elevated levels of cross-resistance in S. marcescens, both log-phase and carbon-starved cultures, depleted of glucose before the onset of high cell-density stationary-phase, were grown in minimal media at either 30 °C or 37 °C and were then challenged for resistance to high temperature (50 °C), low pH (pH 2.8), and oxidative stress (15 mM H2O2). In general, carbon-starved cells exhibited a higher level of resistance to thermal stress, acid stress, and oxidative stress compared to log-phase cells. The extent of carbon-starvation-induced cross-resistance was dependent on incubation temperature and on the particular strain of S. marcescens. In addition, strain- and temperature-dependent variations in long-term starvation survival were also observed. The enhanced stress-resistance of starved S. marcescens cells could be an important factor in their survival and persistence in many non-host environments and within certain host microenvironments where the availability of carbon sources is suboptimal for growth.

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Section: Methodsmentioning

confidence: 99%

Carbon-Starvation Induces Cross-Resistance to Thermal, Acid, and Oxidative Stress in Serratia marcescens

2015

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“…37,38 S. marcescens is one of the pathogenic bacteria and many strains of this bacterium have been sequenced at genome and proteome levels. 10,18,20,22,[39][40][41] To the best of our knowledge, the genome of S. marcescens (ATCC 13880) sequence is available and the proteome of the same is not available till date. 10 Hence, this study was performed to understand the proteome of S. marcescens (ATCC 13880).…”

Section: Discussionmentioning

confidence: 99%

“…10,18,20,22,[39][40][41] To the best of our knowledge, the genome of S. marcescens (ATCC 13880) sequence is available and the proteome of the same is not available till date. 10 Hence, this study was performed to understand the proteome of S. marcescens (ATCC 13880). This strain was initially isolated from pond water and later known to be pathogenic to humans especially children and the immunocompromised patients in the ICU units.…”

Section: Discussionmentioning

confidence: 99%

Proteomic profiling of Serratia marcescens by high-resolution mass spectrometry

2020

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Introduction: Serratia marcescens, an opportunistic human pathogen, is reported as an important cause of nosocomial infection and outbreaks. Although the genome of S. marcescens (ATCC 13880) was completely sequenced by 2014, there are no studies on the proteomic profile of the organism. The objective of the present study is to analyze the protein profile of S. marcescens (ATCC 13880) using a high resolution mass spectrometry (MS). Methods: Serratia marcescens ATCC 13880 strain was grown in Luria-Bertani broth and the protein extracted was subjected to trypsin digestion, followed by basic reverse phase liquid chromatography fractionation. The peptide fractions were then analysed using Orbitrap Fusion Mass Spectrometry and the raw MS data were processed in Proteome Discoverer software. Results: The proteomic analysis identified 15 009 unique peptides mapping to 2541 unique protein groups, which corresponds to approximately 54% of the computationally predicted protein-coding genes. Bioinformatic analysis of these identified proteins showed their involvement in biological processes such as cell wall organization, chaperone-mediated protein folding and ATP binding. Pathway analysis revealed that some of these proteins are associated with bacterial chemotaxis and beta-lactam resistance pathway. Conclusion: To the best of our knowledge, this is the first high-throughput proteomics study of S. marcescens (ATCC 13880). These novel observations provide a crucial baseline molecular profile of the S. marcescens proteome which will prove to be helpful for the future research in understanding the host-pathogen interactions during infection, elucidating the mechanism of multidrug resistance, and developing novel diagnostic markers or vaccine for the disease.

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“…Genes for monofunctional (HPII; katE, 478 aa) and bifunctional (HPI; katG , 731 aa) catalase have been sequenced in Serratia [34, 35]. Presumably, then, both constitutive and inducible forms of catalase operate in Serratia .…”

Section: Discussionmentioning

confidence: 99%

Ozone Sensitivity and Catalase Activity in Pigmented and Non-Pigmented Strains of Serratia Marcescens

Ondarza¹

2017

TOMICROJ

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Background:Ozone exposure rapidly leads to bacterial death, making ozone an effective disinfectant in food industry and health care arena. However, microbial defenses may moderate this effect and play a role in the effective use of oxidizing agents for disinfection. Serratia marcescens is an opportunistic pathogen, expressing genes differentially during infection of a human host. A better understanding of regulatory systems that control expression of Serratia’s virulence genes and defenses is therefore valuable.Objective:Here, we investigated the role of pigmentation and catalase in Serratia marcescens on survival to ozone exposure.Method:Pigmented and non-pigmented strains of Serratia marcescens were cultured to exponential or stationary phase and exposed to 5 ppm of gaseous ozone for 2.5 – 10 minutes. Survival was calculated via plate counts. Catalase activity was measured photometrically and tolerance to hydrogen peroxide was assayed by disk-diffusion.Results:Exposure of S. marcescens to 5 ppm gaseous ozone kills > 90% of cells within 10 minutes in a time and concentration-dependent manner. Although pigmented Serratia (grown at 28°C) survived ozonation better than unpigmented Serratia (grown at 35°C), non-pigmented mutant strains of Serratia had similar ozone survival rates, catalase activity and H2O2 tolerance as wild type strains. Rather, ozone survival and catalase activity were elevated in 6 hour cultures compared to 48 hour cultures.Conclusion:Our studies did not bear out a role for prodigiosin in ozone survival. Rather, induction of oxidative stress responses during exponential growth increased both catalase activity and ozone survival in both pigmented and unpigmented S. marcescens.

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Genome Assembly of Serratia marcescens Type Strain ATCC 13880

Abstract: Serratia marcescens ATCC 13880 is the type strain of the species and a commonly used quality control strain. Here, we present the annotated genome assembly of 5.13 Mbp (59.8% G+C content) as submitted to NCBI under accession no. JOVM00000000.

Cited by 10 publications

References 8 publications

Carbon-Starvation Induces Cross-Resistance to Thermal, Acid, and Oxidative Stress in Serratia marcescens

Carbon-Starvation Induces Cross-Resistance to Thermal, Acid, and Oxidative Stress in Serratia marcescens

Proteomic profiling of Serratia marcescens by high-resolution mass spectrometry

Ozone Sensitivity and Catalase Activity in Pigmented and Non-Pigmented Strains of Serratia Marcescens

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