2016
DOI: 10.1371/journal.pone.0167539
|View full text |Cite
|
Sign up to set email alerts
|

Genome and Proteome Analysis of Rhodococcus erythropolis MI2: Elucidation of the 4,4´-Dithiodibutyric Acid Catabolism

Abstract: Rhodococcus erythropolis MI2 has the extraordinary ability to utilize the xenobiotic 4,4´-dithiodibutyric acid (DTDB). Cleavage of DTDB by the disulfide-reductase Nox, which is the only verified enzyme involved in DTDB-degradation, raised 4-mercaptobutyric acid (4MB). 4MB could act as building block of a novel polythioester with unknown properties. To completely unravel the catabolism of DTDB, the genome of R. erythropolis MI2 was sequenced, and subsequently the proteome was analyzed. The draft genome sequence… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
3
1
1

Citation Types

3
9
0

Year Published

2017
2017
2023
2023

Publication Types

Select...
7
2
1

Relationship

1
9

Authors

Journals

citations
Cited by 15 publications
(12 citation statements)
references
References 86 publications
3
9
0
Order By: Relevance
“…Although stimulation of the oxidative stress response in the dibenzothiophene culture, evident from the upregulation of alkylhydroperoxide reductase, agrees with previous studies ( 22 , 60 ), the upshift in biosynthesis of the low-molecular weight thiols mycothiol and ergothioneine under biodesulfurization conditions has not been reported to date. Accordingly, we propose to extend the list of the sulfur starvation-induced proteins to include enzymes of low-molecular weight thiols biosynthesis.…”
Section: Discussionsupporting
confidence: 90%
“…Although stimulation of the oxidative stress response in the dibenzothiophene culture, evident from the upregulation of alkylhydroperoxide reductase, agrees with previous studies ( 22 , 60 ), the upshift in biosynthesis of the low-molecular weight thiols mycothiol and ergothioneine under biodesulfurization conditions has not been reported to date. Accordingly, we propose to extend the list of the sulfur starvation-induced proteins to include enzymes of low-molecular weight thiols biosynthesis.…”
Section: Discussionsupporting
confidence: 90%
“…), R. erythropolis exhibits an increased resistance to the concomitant strong oxidative stress arising from the toxic substrate decomposition and leading to the accumulation of highly reactive oxygen species, H 2 O 2 , and highly toxic lipid peroxides. Additionally, the induced activity of antioxidant enzymes due to the overexpression of antioxidant-encoding genes (cytochrome P450, catalase, superoxide dismutase, alkyl hydroperoxide reductase, and the protective protein RecA) involved in DNA repair, detoxification of reactive oxygen species, and, finally, in detoxification of pollutants, has been observed [ 99 , 100 ]. Morphological changes occur, particularly changes in size and shape, in addition to an increase in the number of cytoplasmic polyphosphate inclusions (volutin), hypertrophy of individual organelles, and the formation of multicellular aggregates, biofilms, and other spatial structures.…”
Section: Adaptive Cell Modifications Of Rhodococci Exposed To Hydrocarbons and Other Environmental Pollutantsmentioning
confidence: 99%
“…All the previous proteomic investigations, which were performed on Rhodococcus , only highlighted its catabolic behavior towards different organic compounds viz . Fluoranthene 14 , toluene and phenol 15 , propane 16 , triacylglycerol 17 , 4,4′-Dithiodibutyric Acid 18 , benzoate 19 and polyaromatic compounds 20 . In this perspective, the present study for the first time unravels its proteomic response towards the abiotic stress and identified a protein biomarker for low-temperature nitrogen deficient conditions.…”
Section: Discussionmentioning
confidence: 99%