Genome analysis uncovers the prolific antagonistic and plant growth-promoting potential of endophyte Bacillus velezensis K1

Nanjani, Sandhya; Soni, Riteshri; Paul, Dhiraj; Keharia, Haresh

doi:10.1016/j.gene.2022.146671

Cited by 18 publications

(14 citation statements)

References 99 publications

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“…B. velezensis KOF112 had antagonistic activities against gray mold caused by Botrytis cinerea , anthracnose by Colletotrichum gloeosporioides , and downy mildew by Plasmopara viticola [ 44 ]. Currently, a few B. velezensis are commercialized as efficient biocontrol agents, include B. velezensis FZB42, B. velezensis 9912D, B. velezensis SQR9 [ 21 , 42 ]. In Vitro, 6-5 could significantly inhibit the mycelial growth of different physiological races T30-4 and 88069, with inhibition rates of 91.89% and 95.89%, respectively.…”

Section: Discussionmentioning

confidence: 99%

“…Bacillus velezensis K1, an endophytic bacterium originally isolated from aerial roots of Ficus benghalensi , could inhibit 14 different fungal pathogens, including Fusarium oxysporum , Mucor indicus , Sclerotia rolfsii , etc. [ 21 ]. Aspergillus fumigatus LN-4, an endophytic fungus isolated from Melia azedarach , secreted alkaloids that displayed varying degrees of antifungal activities against Fusarium oxysporum , Botrytis cinerea , Colletotrichum gloeosporioides and so on [ 22 ].…”

Section: Introductionmentioning

confidence: 99%

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Isolation and Screening of Antagonistic Endophytes against Phytophthora infestans and Preliminary Exploration on Anti-oomycete Mechanism of Bacillus velezensis 6-5

Zhang

Huang

Hou

et al. 2023

Plants

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Phytophthora infestans, the notorious pathogen of potato late blight, leads to a severe decline in potato yields and even harvest failure. We isolated 201 endophytic isolates from healthy root tissues of potatoes, among which 41 showed strong antagonistic activity against P. infestans. Further, the tolerance to stress and the potential application against potato late blight of these antagonistic isolates were tested. Most of them were extremely tolerant to stresses such as acid–alkali, temperature, UV, salt, and heavy metal stress. However, some antagonistic isolates with excellent stress tolerance might be pathogenic to potatoes. Combining the screening results, a total of 14 endophytes had excellent comprehensive performance in all the tests. In this paper, the endophyte 6-5 was selected among them for the preliminary exploration of the anti-oomycete mechanism. Analysis of the 16S rDNA sequence revealed that 6-5 had a high homology to the corresponding sequence of Bacillus velezensis (99.72%) from the NCBI database. Endophyte 6-5 significantly inhibited the mycelial growth of P. infestans, with an inhibition rate of over 90% in vitro assays, and deformed the hyphal phenotype of P. infestans. In addition, endophyte 6-5 could secrete protease and cellulase, and produce antagonistic substances with high thermal stability, which might be helpful to its antagonistic activity against P. infestans. Furthermore, it was demonstrated that 6-5 had the ability to improve the resistance of potato tubers to late blight. In short, our study described the process of isolating and screening endophytes with antagonistic activity against P. infestans from potato roots, and further explored the potential of biocontrol candidate strain 6-5 in potato late blight control.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Isolation and Screening of Antagonistic Endophytes against Phytophthora infestans and Preliminary Exploration on Anti-oomycete Mechanism of Bacillus velezensis 6-5

Zhang

Huang

Hou

et al. 2023

Plants

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“…Plant-associated bacteria ubiquitously inhabit the rhizosphere (soil-root interface), phyllosphere (aerial surfaces), and endosphere (inside of the plant tissues). These bacteria may contribute to plant growth and health by assisting in nutrient uptake or by suppressing plant pathogens (Vejan et al 2016 ; Afzal et al 2019 ; Saberi Riseh et al 2021 ; Majeed et al 2018 ; Bhardwaj et al 2014 ; Nanjani et al 2022 ). Many studies have demonstrated plant–microbe symbiosis, in which bacteria produce extracellular enzymes or metabolites, thereby improving mineral nutrition and thus impacting plant health and growth, while plants provide energy to those beneficial microbes (Marschner and Rengel 2007 ; Santoyo et al 2016 ; Hardoim et al 2008 ; Zhao et al 2022 ; Thiruvengadam et al 2022 ).…”

Section: Introductionmentioning

confidence: 99%

Genome insights into the plant growth-promoting bacterium Saccharibacillus brassicae ATSA2T

et al. 2023

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Endophytes can facilitate the improvement of plant growth and health in agriculturally important crops, yet their genomes and secondary metabolites remain largely unexplored. We previously isolated Saccharibacillus brassicae strain ATSA2T from surface-sterilized seeds of kimchi cabbage and represented a novel species of the genus Saccharibacillus. In this study, we evaluated the plant growth-promoting (PGP) effect of strain ATSA2T in kimchi cabbage, bok choy, and pepper plants grown in soils. We found a significant effect on the shoot and root biomass, and chlorophyll contents following strain ATSA2T treatment. Strain ATSA2T displayed PGP traits such as indole acetic acid (IAA, 62.9 μg/mL) and siderophore production, and phosphate solubilization activity. Furthermore, genome analysis of this strain suggested the presence of gene clusters involved in iron acquisition (fhuABD, afuABC, fbpABC, and fepCDG) and phosphate solubilization (pstABCHS, phoABHLU, and phnCDEP) and other phytohormone biosynthesis genes, including indole-3-acetic acid (trpABCDEFG), in the genome. Interestingly, the secondary metabolites cerecidin, carotenoid, siderophore (staphylobactin), and bacillaene underlying plant growth promotion were found in the whole genome via antiSMASH analysis. Overall, physiological testing and genome analysis data provide comprehensive insights into plant growth-promoting mechanisms, suggesting the relevance of strain ATSA2T in agricultural biotechnology.

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“…Endophytes, isolated from healthy plants in disease‐problem habitats, may be more resistant to pathogens than introduced species because of their superior environmental adaptation 13 . Endophytes have the ability to antagonize pathogens, induce plant defense and promote plant growth 20–25 . Nowadays, a small number of studies have begun to focus on the screening of endophytes with antagonistic ability against Phytophthora infestans .…”

Section: Introductionmentioning

confidence: 99%

“…13 Endophytes have the ability to antagonize pathogens, induce plant defense and promote plant growth. [20][21][22][23][24][25] Nowadays, a small number of studies have begun to focus on the screening of endophytes with antagonistic ability against Phytophthora infestans. The endophytic fungus Phoma eupatorii isolated from Eupatorium cannabinum has broad-spectrum anti-oomycete activity against nine European Phytophthora infestans isolates.…”

Section: Introductionmentioning

confidence: 99%

Endophytic Bacillus subtilis H17‐16 effectively inhibits Phytophthora infestans, the pathogen of potato late blight, and its potential application

Zhang

Huang

Yang

et al. 2023

Pest Management Science

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BACKGROUNDAs a highly prevalent epidemic disease of potato, late blight caused by Phytophthora infestans poses a serious threat to potato yield and quality. At present, Chemical fungicides are mainly used to control potato late blight, but long‐term overuse of chemical fungicides may lead to environmental pollution and human health threats. Endophytes, natural resources for plant diseases control, can promote plant growth, enhance plant resistance, and secrete antifungal substances. Therefore, there is an urgent need to find some beneficial endophytes to control potato late blight.RESULTSWe isolated a strain of Bacillus subtilis H17‐16 from potato healthy roots. It can significantly inhibit mycelial growth, sporangia germination and the pathogenicity of Phytophthora infestans, induce the resistance of potato to late blight, and promote potato growth. In addition, H17‐16 has the ability to produce protease, volatile compounds (VOCs) and form biofilms. After H17‐16 treatment, most of the genes involved in metabolism, virulence and drug resistance of Phytophthora infestans were down‐regulated significantly, and the genes related to ribosome biogenesis were mainly up‐regulated. Moreover, field and postharvest application of H17‐16 can effectively reduce the occurrence of potato late blight, and the combination of H17‐16 with chitosan or chemical fungicides had a better effect than single H17‐16.CONCLUSIONOur results reveal that Bacillus subtilis H17‐16 has great potential as a natural fungicide for controlling potato late blight, laying a theoretical basis for its development as a biological agent.This article is protected by copyright. All rights reserved.

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Genome analysis uncovers the prolific antagonistic and plant growth-promoting potential of endophyte Bacillus velezensis K1

Cited by 18 publications

References 99 publications

Isolation and Screening of Antagonistic Endophytes against Phytophthora infestans and Preliminary Exploration on Anti-oomycete Mechanism of Bacillus velezensis 6-5

Isolation and Screening of Antagonistic Endophytes against Phytophthora infestans and Preliminary Exploration on Anti-oomycete Mechanism of Bacillus velezensis 6-5

Genome insights into the plant growth-promoting bacterium Saccharibacillus brassicae ATSA2T

Endophytic Bacillus subtilis H17‐16 effectively inhibits Phytophthora infestans, the pathogen of potato late blight, and its potential application

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