Genome Analysis Provides Insights into the Osmoadaptation Mechanisms of Halomonas titanicae

Abstract

Here, we report the osmoadaptation strategies adopted by the halotolerant species Halomonas titanicae BH1(T) inferred from genome sequence analysis. BH strain was isolated in 2010 from a rusticated sample collected in 1991 from the wreck of the Titanic, genome deposited in the database under the accession number (CP059082.1). It showed a high salt tolerance ranging from 0.5 to 25% NaCl (w/v) (optimal growth at 10% NaCl) with no growth in the absence of NaCl. The phylogenomic analysis showed that the BH1 strain is more closely related to the Halomonas sedementi QX-2, a strain isolated from deep-sea sediments. The RAST (Rapid Annotation using Subsystem Technology) annotation revealed divergent mechanisms involved in the primary and secondary response to osmotic stress citing protein implicated in potassium transport, periplasmic glucan synthesis, choline and betaine upake system, biosynthesis of glycine-betaine, ectoine, and proline. These findings provide an overview of the osmoadaptive mechanisms of H. titanicae BH1, and could offer helpful information to future biotechnological applications like osmolyte synthesis and related applications.

In Silico Characterization of Plant Salt Tolerance Promoting KDP Proteins from Alcaligenes xylosoxydans

“…Present study deals with characterization of the kdpFABC complex in the halotolerant bacterium A. xylosoxydans. Multiple studies identified the presence of kdp ATPaseencoding genes in many halotolerant bacteria, but no one has characterized this complex protein (Gumulya et al, 2018;González-Rosales et al, 2022;Najjari, 2023). Therefore, this is the first-ever study reporting the characterization of proteins constituting the kdp ATPase complex.…”