Genome Analysis of Two Novel Lytic Vibrio maritimus Phages Isolated from the Coastal Surface Seawater of Qingdao, China

Han, Yuye; Wang, Min; Liu, Qian; Liu, Huan; Wang, Qi; Duan, Xueping; Liu, Lu; Shao, Hongbing; Guo, Cui

doi:10.1007/s00284-019-01736-2

Cited by 8 publications

(3 citation statements)

References 32 publications

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“…The host bacteria was mixed with the optimal MOI phage, shake and incubate at 37 for 100 min. Next, 200 μL volume was taken at 5,10,15,20,25,30,40,60,90, and 100 min, respectively. Draw one-step growth curve with the potency as the vertical axis and the cultivation time as the horizontal axis, determine the incubation period and cleavage period, and calculate the cleavage amount.…”

Section: Biochemical Characteristics Determinationmentioning

confidence: 99%

“…Lysis amount = final phage titer/initial phage titer. To detect the thermal stability, 1×10 10 PFU/mL bacteriophage solution was added into LB broth at various temperatures (45, 60, and 70), and then aliquots (100 μL) were collected at 5, 10, 15, 20, and 30 min [10] . To determine the pH stability, the phage (100 μL, 2 ×10 9 PFU/mL) was dropped into 900 μL SM at various pH (2-12, adjusted using NaOH or HCl).…”

Section: Biochemical Characteristics Determinationmentioning

confidence: 99%

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Characterization of phage vB_EcoP_HC25 and its therapeutic effect on chicken colibacillosis

Wang,

Li,

Xie

et al. 2023

Preprint

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APEC (avian pathogenic Escherichia coli) infections result in huge economic losses, the emergence of antibiotic resistance bacteria has brought enormous pressure to public health. Bacteriophage therapy has proved to be an attractive candidate for decreasing drug-resistant bacteria. In this paper, a novel Escherichia coli phage vB_EcoP_HC25 was isolated from sewage. Phage vB_EcoP_HC25 had an elongated head, and a short tail, exhibiting the typical features of the rare C3 morphotype phages. The optimal MOI of vB_EcoP_HC25 phage was 0.01, the latency of vB_EcoP_HC25 was 30 min, and approximately 60 min for the lytic phase. The lysis amount was approximately 105 PFU/cell. Phage vB_EcoP_HC25 exhibited wide pH stability (pH 5~11) and good temperature tolerance (< 60℃). In addition, the genomic analysis revealed that phage vB_EcoP_HC25 did not carry any virulence factor genes or antibiotic resistance genes. Moreover, it was found that vB_EcoP_HC25 is a promising candidate phage for biocontrol against antibiotic-resistant Escherichia coli in milk and chicken meat. In addition, the results of the mental state, behavior, serum inflammatory factors, and intestinal morphological analysis indicated that phage vB_EcoP_HC25 was effective to control colibacillosis in chicks infected with E. coli.

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Section: Biochemical Characteristics Determinationmentioning

confidence: 99%

Section: Biochemical Characteristics Determinationmentioning

confidence: 99%

Characterization of phage vB_EcoP_HC25 and its therapeutic effect on chicken colibacillosis

Wang,

Li,

Xie

et al. 2023

Preprint

View full text Add to dashboard Cite

show abstract

“…The precipitate was washed with 10-fold diluted PBS buffer and fixed in a 2% glutaraldehyde solution for 30 min, centrifuged again as before, and the supernatant was discarded, and the precipitate was resuspended in diluted PBS buffer. The phosphotungstic acid negative staining method [20] was used to drop the immobilized Phage168 on a copper mesh, and the morphological structure was observed by 150,000 times transmission electron microscopy.…”

Section: Transmission Electron Microscopymentioning

confidence: 99%

Effect of a Depolymerase Encoded by Phage168 on a Carbapenem-Resistant Klebsiella pneumoniae and Its Biofilm

Sun,

Pu,

Qin

et al. 2023

Pathogens

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Infections caused by carbapenem-resistant Klebsiella pneumoniae (CRKP) are becoming increasingly common within clinical settings, requiring the development of alternative therapies. In this study, we isolated, characterized, and sequenced the genome of a CRKP phage, Phage168. The total genomic DNA of Phage168 was 40,222 bp in length, encoding 49 predicted proteins. Among these proteins, Dep40, the gene product of ORF40, is a putative tail fiber protein that exhibits depolymerase activity based on the result of bioinformatics analyses. In vitro, we confirmed that the molecular weight of the Phage168 depolymerase protein was about 110 kDa, the concentration of the produced phage 168 depolymerase protein was quantified as being 1.2 mg/mL, and the depolymerase activity was still detectable after the dilution of 1.2 µg/mL. This recombinant depolymerase exhibited enzyme activity during the depolymerization of the formed CRKP biofilms. We also found that depolymerase, when combined with polymyxin B, was able to enhance the bactericidal effect of polymyxin B on CRKP strains by disrupting their biofilm. When recombinant depolymerase was used in combination with human serum, it enhanced the sensitivity of the CRKP strain UA168 to human serum, and the synergistic bactericidal effect reached the strongest level when the ratio of depolymerase to human serum was 3:1. Our results indicated that depolymerase encoded by Phage168 may be a promising strategy for combating infections caused by drug-resistant CRKP formed within the biofilm.

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Characterization and Complete Genome Analysis of Pseudomonas aeruginosa Bacteriophage vB_PaeP_LP14 Belonging to Genus Litunavirus

Shi

Zhao

Sun³

et al. 2020

Curr Microbiol

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Genome Analysis of Two Novel Lytic Vibrio maritimus Phages Isolated from the Coastal Surface Seawater of Qingdao, China

Cited by 8 publications

References 32 publications

Characterization of phage vB_EcoP_HC25 and its therapeutic effect on chicken colibacillosis

Characterization of phage vB_EcoP_HC25 and its therapeutic effect on chicken colibacillosis

Effect of a Depolymerase Encoded by Phage168 on a Carbapenem-Resistant Klebsiella pneumoniae and Its Biofilm

Characterization and Complete Genome Analysis of Pseudomonas aeruginosa Bacteriophage vB_PaeP_LP14 Belonging to Genus Litunavirus

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