Genome analysis of Excretory/Secretory proteins in Taenia solium reveals their Abundance of Antigenic Regions (AAR)

Gómez, Sandra María; Adalid‐Peralta, Laura; Palafox-Fonseca, Hector; Cantu-Robles, Vito Adrian; Soberón, Xavier; Sciutto, Edda; Fragoso, Gladis; Bobes, Raúl J.; Laclette, Juan Pedro; Pozo‐Yauner, Luis del; Ochoa-Leyva, Adrián

doi:10.1038/srep09683

Cited by 45 publications

(67 citation statements)

References 65 publications

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“…The AAR value for the nTsKE7 protein was 44.2, as estimated by BepiPred analysis. This value is lower than the previously established AAR value for the antigenic experimental T. solium secretome, 81.3 (24), which suggests a higher density of antigenic regions in the nTsKE7 protein. This result is consistent with the immunogenic properties reported herein for the rTsKE7 protein.…”

Section: Resultscontrasting

confidence: 50%

“…The threshold score was set to 0.35, and only those antigenic regions with 6 or more amino acids in length were selected. Then, an abundance of antigenic regions (AAR) value was calculated to normalize the number of antigenic regions according to the protein length, as previously reported (24).…”

Section: Automated Determination Of Protein Featuresmentioning

confidence: 99%

“…The number of antigenic regions in the nTsKE7 protein was calculated by the BepiPred method (24,41). The BepiPred (v.1.0) method predicts linear epitopes in a protein on the basis of the physicochemical profile of each amino acid (41).…”

Section: Automated Determination Of Protein Featuresmentioning

confidence: 99%

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Experimental and Theoretical Approaches To Investigate the Immunogenicity of Taenia solium-Derived KE7 Antigen

et al. 2017

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Taenia solium cysticercosis, a parasitic disease that affects human health in various regions of the world, is preventable by vaccination. Both the 97-aminoacid-long KETc7 peptide and its carboxyl-terminal, 18-amino-acid-long sequence (GK-1) are found in Taenia crassiceps. Both peptides have proven protective capacity against cysticercosis and are part of the highly conserved, cestode-native, 264-amino-acid long protein KE7. KE7 belongs to a ubiquitously distributed family of proteins associated with membrane processes and may participate in several vital cell pathways. The aim of this study was to identify the T. solium KE7 (TsKE7) fulllength protein and to determine its immunogenic properties. Recombinant TsKE7 (rTsKE7) was expressed in Escherichia coli Rosetta2 cells and used to obtain mouse polyclonal antibodies. Anti-rTsKE7 antibodies detected the expected native protein among the 350 spots developed from T. solium cyst vesicular fluid in a mass spectrometry-coupled immune proteomic analysis. These antibodies were then used to screen a phage-displayed 7-random-peptide library to map B-cell epitopes. The recognized phages displayed 9 peptides, with the consensus motif Y(F/Y)PS sequence, which includes YYYPS (named GK-1M, for being a GK-1 mimotope), exactly matching a part of GK-1. GK-1M was recognized by 58% of serum samples from cysticercotic pigs with 100% specificity but induced weak protection against murine cysticercosis. In silico analysis revealed a universal T-cell epitope(s) in native TsKE7 potentially capable of stimulating cytotoxic T lymphocytes and helper T lymphocytes under different major histocompatibility complex class I and class II mouse haplotypes. Altogether, these results provide a rationale for the efficacy of the KETc7, rTsKE7, and GK-1 peptides as vaccines.

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Section: Resultscontrasting

confidence: 50%

Section: Automated Determination Of Protein Featuresmentioning

confidence: 99%

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Experimental and Theoretical Approaches To Investigate the Immunogenicity of Taenia solium-Derived KE7 Antigen

et al. 2017

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“…We wanted to get the maximum score for each epitope so as to get the most stable structure of mRNA that would lead to the most antigenic epitope for building our immunodiagnostic platform for assessing stress in poultry. Other studies have shown a high performance using this analysis and the determination of the best antigenic epitope relevant for an immunodiagnostic platform, vaccine production, and several treatments, especially in humans (Kolaskar and Tongaonkar, 1990;Hernández-Guzmán et al, 2014;Cai et al, 2015;Gomez et al, 2015). Such results could start the process for understanding of the immunomodulation caused by stress.…”

Section: Resultsmentioning

confidence: 89%

Predicted philogeny, secondary conformational structure, and epitope antigenicity of immunological sequences in poultry

et al. 2017

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ABSTRACT. Poultry production is faced with different types of stresses that are responsible for issues of animal welfare as well as for economic losses. Moreover, the immunity decreases when animals are stressed. In silico analyses are important in reducing the cost and in increasing the accuracy of scientific results. A bioinformatics tool was used to perform ontology studies on 15 different immunological sequences of poultry. The mRNA structures and sequences with maximum antigenic residues were also predicted. No homology was found between the sequences of poultry and mammals. These results helped in the prediction of new potential molecular markers. Of the 15 sequences that were analyzed, predictions could not be made for five because they were longer than 2500 nucleotides; for the remaining 10 sequences, 20 conformational 2 L.J. Lara et al. Genetics and Molecular Research 16 (2): gmr16029423structures per sequence were predicted and the most stable sequences were identified by their minimum free energy values. The highest antigenic epitopes were accepted by the maximum scores; 15 of the total 8934 epitopes that were predicted were analyzed. These results would aid future studies that use synthetic peptides or recombinants as markers or immunomodulators and would expand our understanding on how stress can modulate the immune system. These would also help in developing rapid diagnostic tools, in increasing animal welfare, biosecurity, and productivity, and also in developing of food additives and environmental enrichment for stress control, thereby, making animal production more sustainable.

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“…The more antigenic a protein the more likely it can be used as a biomarker, targeted for immunotherapy, or used in vaccinations. To help prioritize Aspergillus proteins, the Abundance of Antigenic Regions (AAR) 24 value of proteins was calculated using two methods, Kolaskar-Tongaonkar 90 and BepiPred 2.0 91 (Methods). The lower the AAR value the more antigenic the protein 24 .…”

Section: Antigenicity Of the Secretomementioning

confidence: 99%

Comparative systems analysis of the secretome of the opportunistic pathogen Aspergillus fumigatus and other Aspergillus species

Vivek-Ananth

Mohanraj

Vandanashree

et al. 2017

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Aspergillus fumigatus and multiple other Aspergillus species cause a wide range of lung infections, collectively termed aspergillosis. Aspergilli are ubiquitous in environment with healthy immune systems routinely eliminating inhaled conidia, however, Aspergilli can become an opportunistic pathogen in immune-compromised patients. The aspergillosis mortality rate and emergence of drug-resistance reveals an urgent need to identify novel targets. Secreted and cell membrane proteins play a critical role in fungal-host interactions and pathogenesis. Using a computational pipeline integrating data from high-throughput experiments and bioinformatic predictions, we have identified secreted and cell membrane proteins in ten Aspergillus species known to cause aspergillosis. Small secreted and effector-like proteins similar to agents of fungal-plant pathogenesis were also identified within each secretome. A comparison with humans revealed that at least 70% of Aspergillus secretomes has no sequence similarity with the human proteome. An analysis of antigenic qualities of Aspergillus proteins revealed that the secretome is significantly more antigenic than cell membrane proteins or the complete proteome.Finally, overlaying an expression dataset, four A. fumigatus proteins upregulated during infection and with available structures, were found to be structurally similar to known drug target proteins in other organisms, and were able to dock in silico with the respective drug.

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Genome analysis of Excretory/Secretory proteins in Taenia solium reveals their Abundance of Antigenic Regions (AAR)

Cited by 45 publications

References 65 publications

Experimental and Theoretical Approaches To Investigate the Immunogenicity of Taenia solium-Derived KE7 Antigen

Experimental and Theoretical Approaches To Investigate the Immunogenicity of Taenia solium-Derived KE7 Antigen

Predicted philogeny, secondary conformational structure, and epitope antigenicity of immunological sequences in poultry

Comparative systems analysis of the secretome of the opportunistic pathogen Aspergillus fumigatus and other Aspergillus species

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