Genome Analysis and Physiology of Pseudomonas sp. Strain OVF7 Degrading Naphthalene and n-Dodecane

Abstract

The complete genome of the naphthalene- and n-alkane-degrading strain Pseudomonas sp. strain OVF7 was collected and analyzed. Clusters of genes encoding enzymes for the degradation of naphthalene and n-alkanes are localized on the chromosome. Based on the Average Nucleotide Identity and digital DNA–DNA Hybridization compared with type strains of the group of fluorescent pseudomonads, the bacterium studied probably belongs to a new species. Using light, fluorescent, and scanning electron microscopy, the ability of the studied bacterium to form biofilms of different architectures when cultured in liquid mineral medium with different carbon sources, including naphthalene and n-dodecane, was demonstrated. When grown on a mixture of naphthalene and n-dodecane, the strain first consumed naphthalene and then n-dodecane. Cultivation of the strain on n-dodecane was characterized by a long adaptation phase, in contrast to cultivation on naphthalene and a mixture of naphthalene and n-dodecane.

Effects of Polycyclic Aromatic Hydrocarbons on the Composition of the Soil Bacterial Communities in the Tidal Flat Wetlands of the Yellow River Delta of China

“…Many studies reported that some microorganisms can degrade both PAHs and alkanes [ 21 , 22 ]. Moreover, many studies found that some microorganisms included the PAH and alkane degradation genes, such as benzene, toluene, ethylbenzene, xylene, and naphthalene, can be linked to similar genes, and isolated the function microorganisms in different environments [ 23 , 24 ].…”

“…Moreover, many studies found that some microorganisms included the PAH and alkane degradation genes, such as benzene, toluene, ethylbenzene, xylene, and naphthalene, can be linked to similar genes, and isolated the function microorganisms in different environments [ 23 , 24 ]. For example, Ivanova et al reported that Paraburkholderia aromaticivorans strain BN5 included a total of 29 monooxygenase and 54 dioxygenase genes related to the biodegradation of different hydrocarbons [ 21 ]. Therefore, the utilization of the microorganisms that degraded the PAHs has been a focus worldwide.…”