Genome Analysis and Expression Patterns of Odorant-Binding Proteins from the Southern House Mosquito Culex pipiens quinquefasciatus

Pelletier, Julien; Leal, Walter S.

doi:10.1371/journal.pone.0006237

Cited by 87 publications

(94 citation statements)

References 49 publications

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“…In conclusion, our findings demonstrate that the empty neuron system in the ab3 basiconic sensilla of D. melanogaster has some limitations for testing ORs from species of a distant taxon (e.g., Lepidoptera vs. Diptera), although this mutant fruit fly was an enormously successful surrogate for D. melanogaster (10, 11) as well as mosquito ORs (16), probably because flies and mosquitoes, both in the order Diptera, harbor related odorant-binding proteins (21,22). We then tested BmorOR1 in the trichoid sensilla of the fruit fly, which already has a biochemical machinery for the detection of the fruit fly sex pheromone (23,24).…”

Section: Bombykol Elicits Significantly Higher Responses In Flies Thamentioning

confidence: 97%

Bombykol receptors in the silkworm moth and the fruit fly

Syed¹,

Kopp

Kimbrell

et al. 2010

Proc. Natl. Acad. Sci. U.S.A.

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Male moths are endowed with odorant receptors (ORs) to detect species-specific sex pheromones with remarkable sensitivity and selectivity. We serendipitously discovered that an endogenous OR in the fruit fly, Drosophila melanogaster, is highly sensitive to the sex pheromone of the silkworm moth, bombykol. Intriguingly, the fruit fly detectors are more sensitive than the receptors of the silkworm moth, although its ecological significance is unknown. By expression in the "empty neuron" system, we identified the fruit fly bombykol-sensitive OR as DmelOR7a (= DmOR7a). The profiles of this receptor in response to bombykol in the native sensilla (ab4) or expressed in the empty neuron system (ab3 sensilla) are indistinguishable. Both WT and transgenic flies responded with high sensitivity, in a dose-dependent manner, and with rapid signal termination. In contrast, the same empty neuron expressing the moth bombykol receptor, BmorOR1, demonstrated low sensitivity and slow signal inactivation. When expressed in the trichoid sensilla T1 of the fruit fly, the neuron housing BmorOR1 responded with sensitivity comparable to that of the native trichoid sensilla in the silkworm moth. By challenging the native bombykol receptor in the fruit fly with high doses of another odorant to which the receptor responds with the highest sensitivity, we demonstrate that slow signal termination is induced by overdose of a stimulus. As opposed to the empty neuron system in the basiconic sensilla, the structural, biochemical, and/or biophysical features of the sensilla make the T1 trichoid system of the fly a better surrogate for the moth receptor.S ince the identification of bombykol (IUPAC name (10E,12Z)-hexadeca-10,12-dien-1-ol) as a sex pheromone for the silkworm moth, Bombyx mori (1), hundreds of sex pheromones have been identified from lepidopteran species (2). In general, femaleproduced sex pheromones in moths are complex mixtures of straight-chain acetates, aldehydes, and alcohols, with 10-12 carbon atoms and up to three unsaturations (3). This fatty acid-derived semiochemistry (4, 5) is unique to chemical communication in moths, although (7Z)-dodec-7-en-1-yl acetate also has been identified as a sex pheromone for the Asian elephant, Elephas maximus (6). Male moths detect these sex pheromones with such high selectivity and sensitivity that minimal structural modifications render them inactive (7), whereas a single molecule of bombykol is estimated to be sufficient to activate olfactory receptor neurons (ORNs) in male antennae of the silkworm moth (8). These species-specific communication channels seem to have evolved under a strong evolutionary pressure into a sophisticated signal receptor system. In contrast, we have serendipitously identified an ORN housed in the ab4 basiconic sensilla in the antennae of the fruit fly, Drosophila melanogaster, which responds to bombykol with high sensitivity (9), although the ecological significance of this bombykol detector in the fruit fly remains enigmatic. The OR housed in the ab4a neuron was pr...

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Section: Bombykol Elicits Significantly Higher Responses In Flies Thamentioning

confidence: 97%

Bombykol receptors in the silkworm moth and the fruit fly

Syed¹,

Kopp

Kimbrell

et al. 2010

Proc. Natl. Acad. Sci. U.S.A.

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“…The sequencing of the Cx. quinquefasciatus genome has almost been completed (Pelletier andLeal 2009, Waterhouse et al 2008). A large family of olfactory receptor genes (ORs) and gustatory receptor genes (GRs) has been identified (Van der Goes van Naters and Carlson 2006).…”

Section: Van Der Goes Van Naters and Carlson 2006)mentioning

confidence: 99%

“…For example, mechanisms that affect the expression of olfactory genes in mosquitoes can now be studied in detail, allowing for a comprehensive understanding of the insect response to selected odorants (Touhara and Vosshall 2009, Chapter 2 in this volume). In addition, recent data on odorant binding proteins (OBPs) demonstrate the significance of these molecules in transporting volatile chemicals through the sensillum fluid to the receptor membrane, but also how gene silencing, using RNAi technologies, can fundamentally alter these processes, suggesting behavioural changes that went hitherto unexplained (Pelletier and Leal 2009, Zhou et al 2008. The challenge is how this information can be maximally exploited for vector-borne disease control.…”

Section: Introductionmentioning

confidence: 99%

Olfaction in vector-host interactions

Takken¹,

Knols²

2010

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“…Several OBPs have been isolated and cloned from Culex spp. (7)(8)(9), and CquiOBP1 has been mapped to a specific sensilla type on antenna, and functional studies showed binding of this OBP to semiochemicals involved in the chemical ecology of this mosquito species (10).…”

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Acute olfactory response of Culex mosquitoes to a human- and bird-derived attractant

Syed

Leal

2009

Proc. Natl. Acad. Sci. U.S.A.

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West Nile virus, which is transmitted by Culex mosquitoes while feeding on birds and humans, has emerged as the dominant vector borne disease in North America. We have identified natural compounds from humans and birds, which are detected with extreme sensitivity by olfactory receptor neurons (ORNs) on the antennae of Culex pipiens quinquefasciatus (Cx. quinquefasciatus). One of these semiochemicals, nonanal, dominates the odorant spectrum of pigeons, chickens, and humans from various ethnic backgrounds. We determined the specificity and sensitivity of all ORN types housed in different sensilla types on Cx. quinquefasciatus antennae. Here, we present a comprehensive map of all antennal ORNs coding natural ligands and their dose-response functions. Nonanal is detected by a large array of sensilla and is by far the most potent stimulus; thus, supporting the assumption that Cx. quinquefasciatus can smell humans and birds. Nonanal and CO2 synergize, thus, leading to significantly higher catches of Culex mosquitoes in traps baited with binary than in those with individual lures.bird odorants ͉ human odorants across different ethnicities ͉ nonanal-baited traps ͉ semiochemicals ͉ single sensillum recordings

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Genome Analysis and Expression Patterns of Odorant-Binding Proteins from the Southern House Mosquito Culex pipiens quinquefasciatus

Cited by 87 publications

References 49 publications

Bombykol receptors in the silkworm moth and the fruit fly

Bombykol receptors in the silkworm moth and the fruit fly

Olfaction in vector-host interactions

Acute olfactory response of Culex mosquitoes to a human- and bird-derived attractant

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