Geno-toxicity assay of sediment and water samples from the Upper Silesia post-mining areas, Poland by means of Allium-test

Гераськин, С. А.; Oudalova, A.; Michalik, B.; Dikareva, Nina S.; Dikarev, Vladimir G.

doi:10.1016/j.chemosphere.2011.01.008

Cited by 45 publications

(19 citation statements)

References 26 publications

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“…По литературным данным, аберрации хромосомно-го типа (кольца, фрагменты, двойные мосты) считают-ся маркерами радиационного воздействия [19,22,20]. Поскольку хромосомы элодеи канадской очень мелкие (длина -1-2,5 мкм, ширина -0,7-1 мкм), однознач-но идентифицировать фрагменты и двойные мосты было затруднительно даже с использованием иммерсионного объектива × 100.…”

Section: Discussionunclassified

“…Полученные данные по цитогенетическим наруше-ниям в клетках элодеи канадской согласуются с вывода-ми других авторов о различии в реакциях живой клетки на действие факторов нерадиационной и радиационной природы. Считается, что воздействие радиации приводит к возрастанию частоты хромосомных аберраций в клетке (делеции, транслокации), а действие химических мутаге-нов чаще всего приводит к генным мутациям или повре-ждению митотического веретена [13,22,23].…”

Section: Discussionunclassified

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Types of cytogenetic abnormalities in apical root meristem of Elodea сanadensis from Yenisei River areas with different types of anthropogenic pollution

Medvedeva

Bolsunovsky

2016

Ecological genetics

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Background. The spectrum of cytogenetic abnormalities in the apical root meristem of Elodea сanadensis Michx. (elodea) from the Yenisei River was studied depending on the type of anthropogenic pollution. We compared elodea samples from areas with radiation-contaminated sediments (area affected by Rosatom Mining and Chemical Combine, MCC, Zheleznogorsk) and areas with chemical pollution (Krasnoyarsk). Materials and methods. The apical root meristem of elodea was fixed in acetic alcohol (96 % ethanol and glacial acetic acid, 3 : 1) and stained with 1 % aceto-orcein. The cytogenetic analysis of metaphase and ana-telophase elodea cells was carried out with temporal squashed preparations using an Olympus CX31 microscope. Results. At metaphase stage, the predominant types of abnormalities in elodea cells were disoriented chromosomes (up to 9 % of total metaphase cells) and chromosome agglutination (6 %). In the area affected by the MCC an increased content of ring chromosomes in elodea cells was detected, but in terms of frequency of their occurrence no significant differences were revealed between samples from areas with different types of pollution. Among abnormalities at ana-telophase stage, bridges (to 20% of dividing cells) and disoriented chromosomes (up to 8%) dominated. The following abnormalities were also detected: multipolar and asymmetrical mitoses, agglutination and cells with multiple chromosome abnormalities. It was shown that in areas with the highest content of 137Cs in bottom sediments the frequency of cells with bridges and multiple chromosome abnormalities significantly increased as compared to samples from other areas. Conclusion. In the radioactive contamination area of the Yenisei River the spectrum of abnormalities was dominated by chromosome aberrations (bridges, agglutination, chromosome fragmentation) and multiple abnormalities in one and the same cell. In areas with non-radioactive (chemical) contamination of sediments, mitotic spindle irregularities prevailed.

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Section: Discussionunclassified

Types of cytogenetic abnormalities in apical root meristem of Elodea сanadensis from Yenisei River areas with different types of anthropogenic pollution

Medvedeva

Bolsunovsky

2016

Ecological genetics

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“…Chemical maceration of rootlets was done during 1 min in 1N solution of hydrochloric acid. After maceration, the rootlets were placed in aceto-orcein solution for 24 h at [23][24][25] ºС to analyze chromosome aberrations and disorders of cell mitosis.…”

Section: Methodsmentioning

confidence: 99%

Cytogenetic disorders in Triticum aestivum L. cells affected by radionu-clide contamination of water reservoirs in the alienation zone of Chornobyl NPP

Yakymchuk

2018

Biopolym. Cell

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Aim.To study frequency and spectrum of chromosome aberrations in the cells of Triticum aestivum L. root meristem under a prolonged effect of radionuclides from water reservoirs in the proximal alienation zone of the Chernobyl nuclear power plant NPP. Methods. The seeds of two soft winter wheat varieties were treated with samples from water reservoirs in the alienation zone of the Chernobyl NPP. Ana-telophase analysis of chromosome aberrations in crushed cytological preparations of apical meristem of primary rootlets was carried out. Results. Radionuclides from water reservoirs in the proximal alienation zone of the Chornobyl NPP cause an increase in the occurrence of aberrant cells and mitoses by 1.6-4.2 times. The highest level of cytogenetic activity is typical for the radionuclides from the reservoir-cooler of the Chernobyl NPP, Semyhodskyi backwater, drainage N 3 of the NPP and Lake Hlyboke. The obseved aberrations are mostly represented by single and paired acentric fragments, bridges and lagging chromosomes. Conclusion. A prolonged exposure to ionizing radiation by radionuclides from the water reservoirs of the proximal alienation zone of the Chernobyl NPP produces leads to high cytogenetic activity. No correlation between the chromosome aberration level and the scope of specific radionuclides from water reservoirs was found; this may prove the induction of cytogenetic disorders under the radiation effect in the low-rate range. The increased level of aneuploid cells and those with multiple chromosome aberrations confirms genetic сonsequences for the organisms in water reservoirs even with a low specific activity of radionuclides. K e y w o r d s:Aberrations, cytogenetic disorders, radionuclide, low-rate radiation.

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“…Substitution methods are simple to apply [17] and, for situations where LOD values comprise a minor proportion of the total dataset, substitution methods may also be viewed as a pragmatic approach. However, there is little, if any, statistical rationale behind the substitution method 1 and the approach performs worst in situations where there are multiple detection limits [2], which is a situation often encountered in radioecological data sets and especially those obtained from gamma spectrometry [9].…”

Section: Current Norms In Environmental Radioactivity Researchmentioning

confidence: 99%

Limit of detection values in data analysis: Do they matter?

2011

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Abstract. Data sets containing values below the limit of detection (LOD) are known as 'censored data sets'. Such data sets are encountered regularly in most fields of environmental contaminant research. The current norm within environmental radioactivity research is to use substitution methods when analysing data sets that include values below the LOD, commonly replacing each LOD value with a value equal to half the LOD (LOD/2). However, this approach has no statistical basis and has implications when summarising or comparing data sets because it can lead to underestimates or overestimates of both the mean and the standard deviation. To remove the need to apply substitution methods, over the last four decades other fields of environmental science have been adopting statistical techniques developed for medical research applications. Despite the long history of applying these techniques in other fields and two recent environmental radioactivity publications that have used survival analysis techniques, there still seems to be reluctance within the environmental radioactivity research community to adopt these 'new' methods. This paper introduces the statistical techniques that can be used in place of LOD substitution, presents some guidance on the applicability of these techniques for different levels of data censoring and provides some examples of the use of these methods in various contexts. It is hoped the present paper will contribute to the evidence-base supporting the use of survival analysis within the field of environmental radioactivity research and go some way to changing the current norm of substitution using LOD/2.

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Geno-toxicity assay of sediment and water samples from the Upper Silesia post-mining areas, Poland by means of Allium-test

Cited by 45 publications

References 26 publications

Types of cytogenetic abnormalities in apical root meristem of Elodea сanadensis from Yenisei River areas with different types of anthropogenic pollution

Types of cytogenetic abnormalities in apical root meristem of Elodea сanadensis from Yenisei River areas with different types of anthropogenic pollution

Cytogenetic disorders in Triticum aestivum L. cells affected by radionu-clide contamination of water reservoirs in the alienation zone of Chornobyl NPP

Limit of detection values in data analysis: Do they matter?

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