Genital tract infections and infertility

Pellati, Donatella; Mylonakis, Ioannis; Bertoloni, Giulio; Fiore, Cristina; Andrisani, Alessandra; Ambrosini, Guido; Armanini, Decio

doi:10.1016/j.ejogrb.2008.03.009

Cited by 288 publications

(227 citation statements)

References 42 publications

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“…Moreover, bacterial infection could result in epididymitis, which is one of the causes of male infertility, in which sperm function could be impaired through sperm concentration reduction and low motility. 9 The epididymal sperm in the E. coli-challenged mice was analyzed, and results showed that both sperm concentration and motility were significantly decreased in WT mice. The same was also observed in TG mice, but much less severely than normal mice (Figures 4d and e).…”

Section: Resultsmentioning

confidence: 99%

mBin1b transgenic mice show enhanced resistance to epididymal infection by bacteria challenge

et al. 2012

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The mBin1b is a beta-defensin gene identified in the mouse epididymis. In the current report, its expression pattern and antibacterial activities were characterized, and a transgenic (TG) mouse model was developed in which mBin1b was exclusively overexpressed by up to 50-fold over normal levels in the caput epididymis. The experimental animals are healthy with normal reproductive activity, but are more resistant to epididymal infection from Escherichia coli than normal animals. The expression of IL1a and IL1b in the epididymis was decreased in the TG mice, which suggests that mBin1b has a role in the regulation of inflammatory response in the epididymis.

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Section: Resultsmentioning

confidence: 99%

mBin1b transgenic mice show enhanced resistance to epididymal infection by bacteria challenge

et al. 2012

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“…gonorrhoeae es un diplococo gran negativo, aerobio no esporulado e inmóvil, implicado en diferentes infecciones del tracto genitourinario tanto masculino como femenino (1,2). El gonococo, como también suele denominarse frecuentemente a esta bacteria, causa infecciones severas en las mujeres que incluyen la enfermedad pélvica inflamatoria (EPI), endometritis, infección de las trompas de Falopio, abscesos ováricos y peritonitis pélvica (1,3) dejando secuelas a largo plazo como la infertilidad (4). La infección masculina trae como consecuencia la uretritis, prostatitis, epididimitis e infertilidad (5,6).…”

Section: Introductionunclassified

Evaluación in vitro del efecto de Neisseria gonorrhoeae y los factores solubles producto de su metabolismo sobre la calidad espermática

Suárez

Maya

2016

Rev. chil. obstet. ginecol.

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a Microbióloga Bioanalista. b Bacteriólogo. RESUMENAntecedentes: N. gonorrhoeae es una de las principales causas de uretritis, prostatitis y epididimitis en hombres. En el tracto urogenital masculino, esta bacteria o los factores solubles producto de su metabolismo pueden interactuar con los espermatozoides y alterar su calidad. Objetivos: Determinar el efecto de la incubación in vitro de N. gonorrhoeae y los factores solubles producto de su metabolismo sobre la calidad espermática y evaluar la interacción bacteria-espermatozoide. Método: Se realizó incubación de una concentración 0,5 McFarland de N. gonorrhoeae y de los factores solubles producto de su metabolismo bacteriano con muestras de semen de voluntarios aparentemente sanos. Se cuantificaron los parámetros espermáticos convencionales (movilidad y viabilidad) y funcionales (potencial de membrana mitocondrial, integridad y lipoperoxidación de la membrana espermática, detección de especies reactivas del oxígeno, integridad de la cromatina y expresión de Anexina V), empleando microscopía y citometría de flujo, respectivamente. Para evaluar la interacción de N. gonorrhoeae con los espermatozoides humanos se realizaron extendidos en placas para su observación al microscopio. Resultados: N. gonorrhoeae puede unirse al espermatozoide y disminuir la viabilidad espermática luego de 1,5 horas de incubación con los espermatozoides humanos (84,5% vs 66,5%, p<0,05), sin afectar los parámetros espermáticos funcionales. Conclusión: N. gonorrhoeae interactúa con los espermatozoides humanos afectando la viabilidad espermática.PALABRAS CLAVE: Neisseria gonorrhoeae, factores solubles bacterianos, calidad seminal, parámetros espermáticos convencionales y funcionales SUMMARY Background: N. gonorrhoeae is a major cause of urethritis, prostatitis and epididymitis in men. In the male urogenital tract, the bacteria or soluble products of their metabolism may interact with sperm, and alter their quality. Aims: To determine the in vitro effect of incubation of N. gonorrhoeae and soluble products of their metabolism on sperm quality and assess the bacteria-sperm interaction. Methods: Soluble products of bacterial metabolism and 0.5 McFarland concentration of N. gonorrhoeae were incubated with semen samples from healthy volunteers. Conventional (motility and viability) and functional sperm parameters (potential of mitochondrial membrane integrity and sperm membrane lipid peroxidation, detection of reactive oxygen species, chromatin integrity and expression of annexin V) were quantified using microscopy and cytometry flow, respectively. To assess the interaction of N. gonorrhoeae to human sperm microscopic observation was performed. Results: N. gonorrhoeae can join the sperm and decreases sperm viability after 1.5 hours of

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“…Genital tract infection and inflammation have been associated to 8-35% of male infertility cases (1,2,3). Asymptomatic bacteriospermia may play a major role (4,5).…”

Section: Introduction:-mentioning

confidence: 99%