Genital tract dysbiosis in infertile women with a history of repeated implantation failure and pilot study for reproductive outcomes following oral enteric coating lactoferrin supplementation

Kitaya, Kotaro; Ishikawa, Tomomoto

doi:10.1007/s00404-022-06755-2

Cited by 7 publications

(5 citation statements)

References 49 publications

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“…A recent systematic review of lactobacilli-containing vaginal probiotics showed that the concept is promising, but currently available products require improvement, especially in terms of their ability to colonize the female genital tract 45 . To date, two Japanese studies have investigated endometrial microbiota manipulation as a means to improve ART success 46 , 47 . One study investigated combinations of oral and vaginal lactobacilli-containing probiotics and metronidazole 46 , while the other investigated oral lactoferrin supplementation as a prebiotic 47 .…”

Section: Discussionmentioning

confidence: 99%

“…To date, two Japanese studies have investigated endometrial microbiota manipulation as a means to improve ART success 46 , 47 . One study investigated combinations of oral and vaginal lactobacilli-containing probiotics and metronidazole 46 , while the other investigated oral lactoferrin supplementation as a prebiotic 47 . However, the results of these studies are difficult to interpret due to lack of no-intervention control groups.…”

Section: Discussionmentioning

confidence: 99%

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The endometrial microbiota of women with or without a live birth within 12 months after a first failed IVF/ICSI cycle

Bui

Hoogenhuijze

Viveen

et al. 2023

Sci Rep

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The endometrial microbiota composition may be associated with implantation success. However, a ‘core’ composition has not yet been defined. This exploratory study analysed the endometrial microbiota by 16S rRNA sequencing (V1–V2 region) of 141 infertile women whose first IVF/ICSI cycle failed and compared the microbiota profiles of women with and without a live birth within 12 months of follow-up, and by infertility cause and type. Lactobacillus was the most abundant genus in the majority of samples. Women with a live birth compared to those without had significantly higher Lactobacillus crispatus relative abundance (RA) (p = 0.029), and a smaller proportion of them had ≤ 10% L. crispatus RA (42.1% and 70.4%, respectively; p = 0.015). A smaller proportion of women in the male factor infertility group had ≤ 10% L. crispatus RA compared to women in the unexplained and other infertility causes groups combined (p = 0.030). Women with primary infertility compared to secondary infertility had significantly higher L. crispatus RA (p = 0.004); lower proportions of them had ≤ 10% L. crispatus RA (p = 0.009) and > 10% Gardnerella vaginalis RA (p = 0.019). In conclusion, IVF/ICSI success may be associated with L. crispatus RA and secondary infertility with endometrial dysbiosis, more often than primary infertility. These hypotheses should be tested in rigorous well-powered longitudinal studies.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

The endometrial microbiota of women with or without a live birth within 12 months after a first failed IVF/ICSI cycle

Bui

Hoogenhuijze

Viveen

et al. 2023

Sci Rep

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“…There was no difference in pregnancy or miscarriage rates in IVF-ET between women with Lactobacillus -dominant microbiota (>80%) and women with non- Lactobacillus -dominant microbiota [ 22 ]. A study found that the relative dominance rates of Lactobacillus species in uterine endometrium microbiota were not different between women with RIF and control women [ 23 ], and another study also showed that the rates of Lactobacillus species were not different between infertile women with RIF and those with non-RIF [ 24 ]. The results of these studies suggest that the relative dominance rates of Lactobacillus species are not associated with pregnancy outcomes in IVF-ET or RIF.…”

Section: Discussionmentioning

confidence: 99%

Chronic Endometritis and Uterine Endometrium Microbiota in Recurrent Implantation Failure and Recurrent Pregnancy Loss

Takimoto,

Yamada,

Shimada

et al. 2023

Biomedicines

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The aim of this study was to evaluate whether chronic endometritis (CE) and uterine endometrium microbiota were associated with repeated implantation failures (RIFs) and recurrent pregnancy losses (RPLs). In this prospective study, uterine endometrial specimens were obtained from 24 women with RIF, 27 with RPL, and 29 fertile control women. Immunohistochemical staining of CD138 for CE and 16S ribosomal RNA (rRNA) sequencing analysis for uterine endometrium microbiota were performed simultaneously. To assess CE, Liu’s method, McQueen scores and plasma cell count/10 mm2 were used. The frequency of CE (plasma cells > 5.15/10 mm2) was higher in women with RPL (29.6%) than in fertile controls (6.8%, p < 0.05). The plasma cell count/10 mm2 in women with RPL (median 1.53, range 0–252.6, p < 0.01) and women with RIF (median 0.6, range 0–6.98, p < 0.05) was higher than in fertile controls (median 0, range 0–29). The uterine endometrium microbiota in women with RPL or RIF was not significantly different from that in fertile controls. However, the relative dominance rate of Lactobacillus iners (median 4.7%, range 0–99.9 vs. median 0%, range 0–100, p < 0.001) and the positive rate of Ureaplasma species (36.3% vs. 8.6%, p < 0.05) were higher in 11 women with CE than in 69 women without CE. The results suggest that CE may be involved in the pathophysiology of RPL and RIF. Lactobacillus iners and Ureaplasma species may be associated with the etiology of CE.

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“…The disruption of the healthy uterine microbiome is thought to result in chronic endometritis [ 77 ] and triggers a pro-inflammatory response that renders the endometrium unfavorable for implantation [ 22 , 32 , 80 ]. What we need to mention, however, is the fact that there are studies that failed to report a difference between the women with dysbiotic and eubiotic communities as far as the IVF success rates are concerned [ 55 , 81 ]. Finally, there are even reports in which there is no difference in the microbial composition between the women who underwent IVF and got pregnant and those who did not.…”

Section: Microbiota and Rifmentioning

confidence: 99%

Understanding the Role of Female Genital Tract Microbiome in Recurrent Implantation Failure

Lafioniatis,

Samara,

Makaritsis

et al. 2024

JCM

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The realization of the role of the microbiome of the female reproductive tract in health and disease has opened numerous possibilities for the scientific examination of the intertwining role between the human host and its microbiota. The imbalance in the composition of the microbial communities of the vagina and uterus is now recognized as a risk factor for many complications in pregnancy and according to the data from numerous studies, it is possible for this imbalance to play a crucial role in creating a hostile endometrial environment, and therefore, contributing to the etiology of recurrent implantation failure. Nevertheless, our current understanding of these complicated biological phenomena is far from complete, and in the future, there needs to be a systematic and thorough investigation of the diagnosis and therapy of this condition. This will enable scientists who engage in the field of assisted reproduction technologies to accurately identify and cure women in whom dysbiosis hinders the achievement of a healthy pregnancy.

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Genital tract dysbiosis in infertile women with a history of repeated implantation failure and pilot study for reproductive outcomes following oral enteric coating lactoferrin supplementation

Cited by 7 publications

References 49 publications

The endometrial microbiota of women with or without a live birth within 12 months after a first failed IVF/ICSI cycle

The endometrial microbiota of women with or without a live birth within 12 months after a first failed IVF/ICSI cycle

Chronic Endometritis and Uterine Endometrium Microbiota in Recurrent Implantation Failure and Recurrent Pregnancy Loss

Understanding the Role of Female Genital Tract Microbiome in Recurrent Implantation Failure

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