Genital papillomavirus in Greek women with high-grade cervical intraepithelial neoplasia and cervical carcinoma

Abstract

Fifty biopsies from high-grade squamous intraepithelial lesions (HG-SIL) and 14 cervical carcinoma biopsies from Greek women were screened for the presence of human papillomavirus (HPV) DNA sequences by Southern blot hybridization and by the polymerase chain reaction (PCR) for the presence of different HPV types. In high-grade SIL, HPV DNA sequences were detected in 44 of 50 biopsies with the following distribution: 36% HPV 16, 12% HPV 18, 6% HPV 31, 6% HPV 33, 4% HPV 51, and 24% unclassified HPV types. In cervical carcinoma biopsies, 13 of 14 specimens were positive for HPV DNA sequences. Six biopsies were positive for HPV 16, three were positive for HPV 18, and four contained unclassified HPV types. Overall, of the total 64 biopsies, 57 (89%) were positive for HPV DNA sequences. Of these, Southern blot hybridization alone detected HPV DNA sequences in 39 cases, whereas by PCR 18 additional specimens were found to be positive for HPV. Among the HPV 16-positive biopsies, two samples produced a Pstl banding pattern very similar but not identical to that of HPV 16 prototype and were referred to as HPV 16 isolates. One HPV 16 isolate appears to carry a mutation within the carboxy-terminal half of the L2 gene that results in the loss of a Pstl site. The other HPV 16 isolate had a similar Pstl banding pattern to that previously reported as HPV 16 "variant" found in Cape Town [Williamson et al., 1989, Journal of Medical Virology 28: 146-149, 1994, Journal of Medical Virology 43: 231-237.] and in Italy [Li Vigni et al., 1994, 2nd International Congress of Papillomavirus in Human Pathology (Abstracts), p 100.].

Detection of human papillomaviruses in squamous cell carcinomas of the lung

“…To extract DNA from frozen specimens, small pieces taken from the tumour specimens were minced well and incubated overnight at 55°C in 10 mM Tris-HCl, pH 8.0, 10 mM NaCl, 0.25% SDS and 200 µg/ml proteinase K. The DNA was extracted with phenol/chloroform, precipitated and resuspended in dH 2 O [14,15].…”

“…HPV DNA is detected in approximately 10% of all human neoplasms; such viruses may, then, be related to the aetiology of a number of tumours [20]. There is strong support for the role of specific HPV types in the pathogenesis of squamous cell carcinoma of the anogenital tract, and more than 90% of cervical carcinomas contain HPV DNA, usually of the high-oncogenic-risk HPV types 16 and 18 and less frequently of intermediate-risk HPV types 31, 33 and 35 [15,38]. DNAs from high-risk HPV types are frequently integrated into genomic DNA, and E6/E7 oncoproteins accelerate the cell cycle by inactivation of tumour suppressor proteins [39].…”