Genistein synergizes centchroman action in human breast cancer cells

Kaushik, Shweta; Shyam, Hari; Sharma, Rishi; Balapure, Anil K.

doi:10.4103/0253-7613.194852

Cited by 29 publications

(9 citation statements)

References 24 publications

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“…In contrast, MDA-MB-231 cells are estrogen-independent. 21 In this study, we used the breast CSCs of MDA-MB-231 and MCF-7 cell as cell models for studying the effects of miR-628 on migration and invasion.…”

Section: Discussionmentioning

confidence: 99%

MicroRNA 628 suppresses migration and invasion of breast cancer stem cells through targeting SOS1

Lin¹,

Gao²,

Yan³

et al. 2018

OTT

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PurposeThe purpose of this study is to evaluate the effects of miR-628 on migration and invasion of breast cancer stem cells (CSCs), which are essential for tumor recurrence and metastasis.Materials and methodsQuantitative reverse transcription-polymerase chain reaction was used to determine the expression of microRNAs and mRNAs. A subpopulation of CD44+/CD24− breast CSCs were sorted by flow cytometry. Transwell assays were used to evaluate cell migration and invasion. Luciferase reporter assays were performed to verify whether miR-628 targeted SOS Ras/Rac guanine nucleotide exchange factor 1 (SOS1). pcDNA3.1(+)-SOS1 was constructed for overexpressing SOS1 after transfection.ResultsCompared with primary breast cancer cells, bone metastatic breast cancer cells showed significant downregulation of miR-628. The CD44+/CD24− breast CSC subpopulations in MDA-MB-231 and MCF-7 cell lines were analyzed and sorted. Transfection with an miR-628 mimic significantly suppressed the migration and invasion of these breast CSCs by targeting SOS1, which plays an essential role in epithelial-to-mesenchymal transition. Overexpression of SOS1 rescued miR-628-mediated migration and invasion by upregulating Snail and vimentin, and downregulating E-cadherin.ConclusionmiR-628 suppressed migration and invasion of breast CSCs of MDA-MB-231 and MCF-7 cells by directly targeting SOS1. Enhancement of miR-628 expression might be an effective strategy for managing breast cancer metastasis.

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Section: Discussionmentioning

confidence: 99%

MicroRNA 628 suppresses migration and invasion of breast cancer stem cells through targeting SOS1

Lin¹,

Gao²,

Yan³

et al. 2018

OTT

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“…MDA-MB-231 and MCF-7 human cancer cell lines are estrogen receptor and estrogen receptor-positive cells, respectively. These cell lines are well-established in vitro models for evaluating estrogen-responsive or estrogen-independent antineoplastic drugs [ 3 ].…”

Section: Introductionmentioning

confidence: 99%

Antiproliferative Activity of Combined Biochanin A and Ginsenoside Rh2 on MDA-MB-231 and MCF-7 Human Breast Cancer Cells

et al. 2018

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Breast cancer is the most frequently diagnosed cancer in women worldwide. The antiproliferative activities of biochanin A (BA) and ginsenoside Rh2 were determined by evaluating their inhibitory effect on MDA-MB-231 human breast cancer cell proliferation. The combination of BA with Rh2 was also assessed. In MDA cells, combination treatment led to a decrease in the EC50 values of BA and Rh2 to 25.20 μM and 22.75 μM, respectively. In MCF-7 cells, the EC50 values of combined BA and Rh2 decreased to 27.68 μM and 25.41 μM, respectively. BA combined with Rh2 also improved the inhibition of MDA-MB-231 and MCF-7 cell migration and invasion compared to the individual compounds. Western blot analysis demonstrated upregulation in p-p53, p-p38, and p-ASK1 proteins while levels of TRAF2 were downregulated. These results suggest that BA combined with Rh2 exhibits synergistic effects against MDA-MB-231 and MCF-7 cell proliferation.

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“…Genistein sensitized large cell lymphoma to chemotherapeutic agents such as cyclophosphamide, doxorubicin, vincristine, and prednisone [ 333 ]. Additionally, co-administration of genistein with drugs such as methotrexate, eicosapentaenoic acid, topotecan, centchroman, and other phytochemicals such as capsaicin showed synergistic effects in TNBC cells and other cancers, indicative of better efficacy in targeting multiple oncogenic pathways using drug combinations when compared to drug monotherapy [ 322 , 334 , 335 , 336 , 337 ].…”

Section: Phytochemicals As Anticancer Compounds Effective In the Tmentioning

confidence: 99%

The “Yin and Yang” of Natural Compounds in Anticancer Therapy of Triple-Negative Breast Cancers

Varghese

Samuel

Abotaleb

et al. 2018

Cancers

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Among the different types of breast cancers, triple-negative breast cancers (TNBCs) are highly aggressive, do not respond to conventional hormonal/human epidermal growth factor receptor 2 (HER2)-targeted interventions due to the lack of the respective receptor targets, have chances of early recurrence, metastasize, tend to be more invasive in nature, and develop drug resistance. The global burden of TNBCs is increasing regardless of the number of cytotoxic drugs being introduced into the market each year as they have only moderate efficacy and/or unforeseen side effects. Therefore, the demand for more efficient therapeutic interventions, with reduced side effects, for the treatment of TNBCs is rising. While some plant metabolites/derivatives actually induce the risk of cancers, many plant-derived active principles have gained attention as efficient anticancer agents against TNBCs, with fewer adverse side effects. Here we discuss the possible oncogenic molecular pathways in TNBCs and how the purified plant-derived natural compounds specifically target and modulate the genes and/or proteins involved in these aberrant pathways to exhibit their anticancer potential. We have linked the anticancer potential of plant-derived natural compounds (luteolin, chalcones, piperine, deguelin, quercetin, rutin, fisetin, curcumin, resveratrol, and others) to their ability to target multiple dysregulated signaling pathways (such as the Wnt/β-catenin, Notch, NF-κB, PI3K/Akt/mammalian target of rapamycin (mTOR), mitogen-activated protein kinase (MAPK) and Hedgehog) leading to suppression of cell growth, proliferation, migration, inflammation, angiogenesis, epithelial-mesenchymal transition (EMT) and metastasis, and activation of apoptosis in TNBCs. Plant-derived compounds in combination with classical chemotherapeutic agents were more efficient in the treatment of TNBCs, possibly with lesser side effects.

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Genistein synergizes centchroman action in human breast cancer cells

Cited by 29 publications

References 24 publications

MicroRNA 628 suppresses migration and invasion of breast cancer stem cells through targeting SOS1

MicroRNA 628 suppresses migration and invasion of breast cancer stem cells through targeting SOS1

Antiproliferative Activity of Combined Biochanin A and Ginsenoside Rh2 on MDA-MB-231 and MCF-7 Human Breast Cancer Cells

The “Yin and Yang” of Natural Compounds in Anticancer Therapy of Triple-Negative Breast Cancers

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