Genistein suppresses the inflammation and GSK-3 pathway in an animal model of spontaneous ovarian cancer

Abstract

Background/aim: Numerous studies show that cancer risk is reduced by consumption of soy-based foods containing genistein, but its effects on the glycogen synthase kinase-3 pathway (GSK-3) in ovarian cancer is unknown. Therefore, we tested the properties of genistein on inflammatory biomarkers and GSK-3 signaling pathways in the ovaries of old laying hens with ovarian cancer. Materials and methods: A total of 300 laying hens were distributed into three groups as follows: group 1, animals fed a standard diet (comprising 22.39 mg of genistein/kg of diet); groups 2 and 3, animals fed a standard diet reconstituted with supplementation of 400 mg or 800 mg of genistein/kg of diet, respectively. Results: Genistein modulated the inflammatory biomarkers by decreasing serum tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), interleukin-8 (IL-8), and vascular endothelial growth factor (VEGF) compared with control (P < 0.001). Moreover, genistein up-regulated insulin receptor substrate-1 (p-IRS-1) and protein kinase B (p-AKT), but down-regulated GSK-3α and β after treatment. Genistein acts in a dosedependent manner. Conclusion: Genistein exhibited an anti-cancer effect by reducing pro-inflammatory biomarkers levels and inhibiting GSK-3 expression in the ovaries of old laying hens. Genistein is a potential candidate in the chemoprevention and/or treatment of ovarian cancer.

Immunosuppressive activity is attenuated by Astragalus polysaccharides through remodeling the gut microenvironment in melanoma mice

“…Previous studies suggested that daidzein and genistein could exert antitumor effects. 50 , 51 However, here, most of these two metabolites in the APS group were downregulated compared with the other three groups. Therefore, it may not be through them that APS exert antitumor effects.…”

Understanding the mechanism of Trikatu in type 2 diabetes mellitus and lipid-related metabolic disorders: A network pharmacology approach

“…Genistein also protects pancreatic beta cells and improve insulin secretion, as well as regulate insulin resistance via the PI3K and AMPK pathways (63–66). Moreover, literature from other diseases like cancer provides empirical evidence that genistein regulates GSK3B in cell proliferation (67, 68). This study also supports the notion that genistein regulates GSK3B in the insulin resistance pathway.…”

Traditional Chinese Medicines as Effective Reversals of Epithelial-Mesenchymal Transition Induced-Metastasis of Colorectal Cancer: Molecular Targets and Mechanisms

“…Genistein, a beneficial soy-derived isoflavone and is well known for its various biological activities, such as the inhibition of inflammatory, promotion of apoptosis, anti-proliferative, and anti-bacterial effects (Erten et al, 2021). Convincing evidence has shown that genistein can repress the proliferation and induce apoptosis of CRC cells (Shafiee et al, 2016;Liang et al, 2018;.…”