Genistein reduces the noxious effects of in utero bisphenol A exposure on the rat prostate gland at weaning and in adulthood

Bernardo, Bruna Dias; Brandt, Joyce Zalotti; Grassi, Thiago Luís Magnani; Silveira, Lívia Teresa Ribeiro da; Scarano, Wellerson Rodrigo; Barbisan, Luı́s Fernando

doi:10.1016/j.fct.2015.07.011

Cited by 31 publications

(31 citation statements)

References 60 publications

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“…Early exposure to weak and strong oestrogens can induce adverse changes in prostate development. A recent study revealed an increased incidence of multifocal inflammation and reactive hyperplasia in Sprague Dawley rat ventral prostate at 6 months of age after prenatal exposure to 25 and 250 μg/kg bw/day of BPA (Bernardo et al ., ). In contrast to the current study, BPA exposure induced increased ventral prostate weight, increased proliferation of epithelial cells and changes in distribution of compartments at PD 21 (Bernardo et al ., ).…”

Section: Discussionmentioning

confidence: 97%

“…A recent study revealed an increased incidence of multifocal inflammation and reactive hyperplasia in Sprague Dawley rat ventral prostate at 6 months of age after prenatal exposure to 25 and 250 μg/kg bw/day of BPA (Bernardo et al ., ). In contrast to the current study, BPA exposure induced increased ventral prostate weight, increased proliferation of epithelial cells and changes in distribution of compartments at PD 21 (Bernardo et al ., ). It is possible that different effects arise after pre‐ and postnatal exposures, as no effects on inflammation in adult ventral prostate were seen in the low‐dose group of the current study, and as a study on neonatal exposure to BPA revealed no pathological changes at 6 months of age (Ho et al ., ).…”

Section: Discussionmentioning

confidence: 97%

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Low‐dose effect of developmental bisphenol A exposure on sperm count and behaviour in rats

et al. 2016

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SUMMARYBisphenol A is widely used in food contact materials and other products and is detected in human urine and blood. Bisphenol A may affect reproductive and neurological development; however, opinion of the European Food Safety Authority (EFSA) on bisphenol A (EFSA J, 13, 2015 and 3978) concluded that none of the available studies were robust enough to provide a point of departure for setting a tolerable daily intake for bisphenol A. In the present study, pregnant Wistar rats (n = 17-21) were gavaged from gestation day 7 to pup day 22 with bisphenol A doses of 0, 25 lg, 250 lg, 5 mg or 50 mg/kg bw/day. In the offspring, growth, sexual maturation, weights and histopathology of reproductive organs, oestrus cyclicity and sperm counts were assessed. Neurobehavioural development was investigated using a behavioural testing battery including tests for motor activity, sweet preference, anxiety and spatial learning. Decreased sperm count was found at the lowest bisphenol A dose, that is 25 lg/kg/day, but not at the higher doses. Reproductive organ weight and histology were not affected and no behavioural effects were seen in male offspring. In the female offspring, exposure to 25 lg/kg bw/day bisphenol A dose resulted in increased body weight late in life and altered spatial learning in a Morris water maze, indicating masculinization of the brain. Decreased intake of sweetened water was seen in females from the highest bisphenol A dose group, also a possible sign of masculinization. The other investigated endpoints were not significantly affected. In conclusion, the present study using a robust experimental study design, has shown that developmental exposure to 25 lg/kg bw/day bisphenol A can cause adverse effects on fertility (decreased sperm count), neurodevelopment (masculinization of spatial learning in females) and lead to increased female body weight late in life. These results suggest that the new EFSA temporary tolerable daily intake of 4 lg/kg bw/day is not sufficiently protective with regard to endocrine disrupting effects of bisphenol A in humans.

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Section: Discussionmentioning

confidence: 97%

Section: Discussionmentioning

confidence: 97%

Low‐dose effect of developmental bisphenol A exposure on sperm count and behaviour in rats

et al. 2016

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“…This difference has been partially associated with Asian diets that consist of a considerably large proportion of phytoestrogens [20, 21]. Some phytoestrogens can reduce the carcinogenic effect of bisphenol A by blocking the connection of bisphenol A and ER [23, 24]. Epidemiologic and experimental evidence suggest that phytoestrogens play a chemopreventive role in breast cancers.…”

Section: Discussionmentioning

confidence: 99%

Spatholobus suberectusColumn Extract Inhibits Estrogen Receptor Positive Breast Cancer via Suppressing ER MAPK PI3K/AKT Pathway

Sun

Zhang

et al. 2016

Evidence-Based Complementary and Alternative Medicine

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Although Chinese herbal compounds have long been alternatively applied for cancer treatment in China, their treatment effects have not been sufficiently investigated. The Chinese herb Spatholobus suberectus is commonly prescribed to cancer patients. HPLC analysis has shown that the main components of Spatholobus suberectus are flavonoids that can be classified as phytoestrogens, having a structure similar to estrogen. This study was designed to investigate the effects of Spatholobus suberectus column extract (SSCE) on the estrogen receptor-positive (ER+) breast cancer cell line MCF-7 and its possible molecular mechanism. In our study, MTT assay was performed to evaluate cell viability. The results show that SSCE (80, 160, and 320 μg/ml) significantly decreased the viability of MCF-7 cells. SSCE also triggered apoptosis, arrested the cell cycle at the G0/G1 phase, and inhibited cell migration. A dual-luciferase reporter system showed that SSCE suppressed intranuclear p-ER activity; Western blot analysis confirmed the repressed expression of phosphorylated-ER alpha (p-ERα), ERK1/2, p-ERK1/2, AKT, p-AKT, p-mTOR, PI3K, and p-PI3K, indicating that SSCE suppressed the MAPK PI3K/AKT signaling pathway. Collectively, our results suggest that SSCE causes apoptosis, an arrest in the G0/G1 phase, and a decrease in migration in ER+ MCF-7 cells via hypoactivity of the ER and suppression of the MAPK PI3K/AKT pathway.

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“…In agreement with our finding, Piotrowska et al (2011) did not find any change in testosterone levels of rats treated with soy isoflavones despite the high dose used and the fact that exposure was during the critical prenatal life to sexual maturity period. In general, some studies have implied that there is an antioxidant potential role of soy isoflavones for improving the inflammatory and oxidative effects of BPA on the gonad (Zhang et al 2014;Bernardo et al 2015), and thus soy isoflavones can maintain proper function of the male reproductive system. In contrary, a few studies have also indicated isoflavones reduced fertility and sperm quality along with a possibly antiandrogenic effect (Meena et al 2017) that cannot be ignored in this context.…”

Section: Discussionmentioning

confidence: 99%

Co-exposure to endocrine disruptors: effect of bisphenol A and soy extract on glucose homeostasis and related metabolic disorders in male mice

Veissi

Jafarirad

Ahangarpour

et al. 2018

Endocrine Regulations

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Genistein reduces the noxious effects of in utero bisphenol A exposure on the rat prostate gland at weaning and in adulthood

Cited by 31 publications

References 60 publications

Low‐dose effect of developmental bisphenol A exposure on sperm count and behaviour in rats

Low‐dose effect of developmental bisphenol A exposure on sperm count and behaviour in rats

Spatholobus suberectusColumn Extract Inhibits Estrogen Receptor Positive Breast Cancer via Suppressing ER MAPK PI3K/AKT Pathway

Co-exposure to endocrine disruptors: effect of bisphenol A and soy extract on glucose homeostasis and related metabolic disorders in male mice

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