Genistein improve nicotine toxicity on male mice pancreas

Abstract

Nicotine is the most toxic factor of tobacco. Genistein is a phytoestrogen and antioxidant that has numerous health benefits. The aim of this study is to evaluate the effects of genistein against toxic properties of nicotine to the pancreas of mice. For this purpose, 48 male mice were randomly assigned into six groups (n=8): normal control, nicotine control (2.5 mg/kg), genistein (25 and 50 mg/kg), and nicotine+genistein (25 and 50 mg/kg) treated groups. Various doses of genistein and genistein+nicotine were administered intraperitoneally to animals for 4 weeks. The weight of pancreas, total antioxidant capacity and nitrite oxide of serum, insulin levels, and the number and diameter of islets of Langerhans were investigated. Nicotine administration reduced significantly total antioxidant capacity, insulin, pancreas weight, and the number and diameter of islets of Langerhans and increased nitrite oxide in serum compared to the control normal group ( P <0.05). Conversely, genistein and genistein+nicotine increased significantly insulin, total antioxidant capacity, and the number and diameter islets of Langerhans and decreased serum nitrite oxide compared to the nicotine control group. It seems that the genistein can improve pancreas damage following the nicotine administration.

Molecular Mechanisms of Action of Genistein in Cancer: Recent Advances

“…Further, genistein is known as a strong antioxidant compound and can provide protection against oxidative stress in various noncancerous tissues. For instance, treatment with genistein was shown to preserve follicular quality in rats with polycystic ovary syndrome by elevating antioxidant capacity (Rajael et al, 2019), increased the total antioxidant activities and reduced kidney damages in nephrotic rats (Javanbakht et al, 2014), or improved antioxidant status and decreased pancreatic damage following administration of nicotine to mice (Salahshoor et al, 2019). In concert with its multitargeted anticancer activities, protective action of genistein on adjacent normal healthy tissues makes this natural isoflavone an attractive key compound for development of novel and more effective drug for combating malignant disorders in the future.…”

Nicotine impact on rat substantia nigra compacta

“…Moreover, plasma TAC level decreased in bidi workers as per exposure to nicotine [ 33 ], and the ROS levels increased in brain regions concerned with reward after recurrent exposure to a variety of misused drugs, including nicotine [ 34 ]. In addition, it has been reported that nicotine (2.5 mg/kg), reduced the total serum antioxidant capacity level with growth in the rate of ROS from nicotine at pancreases strictures of mice [ 35 ].…”

Radioprotective Effects of Allium jesdianum Extract on Reduction of Pancrease Damages Following γ-Radiation through Down-regulation of Apoptotic Genes, Antioxidants Regulation, and Suppression of Inflammatory Markers

“…Since the pancreas is involved in the regulation of blood glucose, it is considered a vital organ (19). Nowadays, the rate of cancer epidemy is higher than ever.…”

“…The produced dye in the commercial kit was read at 532 nm. Finally, the MDA level was presented as nmol/mg of protein (19).…”

“…The glucose oxidase (GOD-PAP) technique was applied to assess serum glucose levels. Also, serum insulin was calculated by PLR ELISA (USA, Sigma) mouse kit (19).…”