Genistein and daidzein treatments differently affect uterine homeostasis in the ovary-intact middle-aged rats

Jarić, Ivana; Živanović, Jasmina; Miler, Marko; Ajdžanović, Vladimir; Blagojević, Duško; Ristić, Nataša; Milošević, Verica; Nestorović, Nataša

doi:10.1016/j.taap.2017.12.001

Cited by 15 publications

(10 citation statements)

References 65 publications

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“…Contrariwise, a significant increase in the percentage of PCNA positive cells was observed in granulosa cells of irradiated rats treated with either GEN or E2 as compared to the untreated irradiated group. These data support a recent study demonstrating the proliferative effect of GEN on rat uterus 51 . The proliferative effects of GEN or E2 could be attributed to increased AMH levels which could act as a guardian to control the balance between proliferation and differentiation of germ cells 52 .…”

Section: Discussionsupporting

confidence: 92%

Phytoestrogen genistein hinders ovarian oxidative damage and apoptotic cell death-induced by ionizing radiation: co-operative role of ER-β, TGF-β, and FOXL-2

Haddad¹,

Said

Kamel

et al. 2020

Sci Rep

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Radiotherapy is a well-known cause of premature ovarian failure (POF). Therefore, we investigated the molecular influence of genistein (GEN) on the ovarian reserve of rats exposed to ϒ-radiation. Female Sprague Dawley rats were exposed to a 3.2 Gy γ-radiation to induce POF and/or treated with either GEN (5 mg/kg, i.p.) or Ethinyl estradiol (E2; 0.1 mg/kg, s.c.), once daily for 10 days. GEN was able to conserve primordial follicles stock and population of growing follicles accompanied with reduction in atretic follicles. GEN restored the circulating estradiol and anti-Müllerian hormone levels which were diminished after irradiation. GEN has potent antioxidant activity against radiation-mediated oxidative stress through upregulating endogenous glutathione levels and glutathione peroxidase activity. Mechanistically, GEN inhibited the intrinsic pathway of apoptosis by repressing Bax expression and augmenting Bcl-2 expression resulted in reduced Bax/Bcl-2 ratio with subsequent reduction in cytochrome c and caspase 3 expression. These promising effects of GEN are associated with improving granulosa cells proliferation. On the molecular basis, GEN reversed ovarian apoptosis through up-regulation of ER-β and FOXL-2 with downregulation of TGF-β expression, therefore inhibiting transition of primordial follicles to more growing follicles. GEN may constitute a novel therapeutic modality for safeguarding ovarian function of females’ cancer survivors.

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Section: Discussionsupporting

confidence: 92%

Phytoestrogen genistein hinders ovarian oxidative damage and apoptotic cell death-induced by ionizing radiation: co-operative role of ER-β, TGF-β, and FOXL-2

Haddad¹,

Said

Kamel

et al. 2020

Sci Rep

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“…These results were in agreement with studies where the uterus weight was increased in ovary-intact middle-aged rats (Jarić et al. 2018) and ovariectomized mice (Ishimi et al. 2000; Zhang et al.…”

Section: Discussionsupporting

confidence: 93%

Differential effects of genistein and 8-prenylgenistein on reproductive tissues in immature female mice

Sui

Lin

et al. 2019

Pharmaceutical Biology

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Context: We identified an active prenylated derivative of genistein, 8-prenylgenistein (8PG) from Erythrina variegata L. (Leguminosae) and found that 8PG increased osteoprotective effects of genistein in oestrogen-deficient mice. Objective: This study investigated and compared the oestrogenic effects of genistein and 8PG on uterus and vagina of immature mice. Materials and methods: Immature female CD-1 mice were orally treated with vehicle (Control, n = 10) or genistein (75 mg/kg, n = 10) or 8PG with low (8PG-L, 75 mg/kg, n = 10) and high dose (8PG-H, 150 mg/kg, n = 10) for 7 consecutive days by intragastric gavage. The uterus and vagina were harvested for histological and molecular measurements. Results: Treatment with genistein and 8PG-H significantly increased uterus index (1.98 ± 0.21 & 1.49 ± 0.16 mg/g) and vagina index (3.83 ± 0.11 & 3.13 ± 0.25 mg/g) as compared to untreated control (uterus, 1.12 ± 0.13 mg/g; vagina, 2.32 ± 0.18 mg/g). Accordingly, both genistein and 8PG-H made vaginal cells keratinized and induced uterine and vaginal hypertrophy associated with the endometrial proliferation. 8PG-L did not affect oestrus cycle and histology of uterus and vagina. Treatment of immature mice with genistein or 8PG-H upregulated protein expression of oestrogen receptor-α (ER-α) and proliferating cell nuclear antigen (PCNA), but 8PG-L did not alter ER-α and PCNA expression in uterus and vagina. Conclusion: This study indicated that 8-prenylgenistein exerted oestrogenic effects in immature female mice. The efficacy and safety of 8-prenylgenistein when applied in improving oestrogen deficiency-induced syndrome requires further elucidation.

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“…The smaller increase in virus activity observed in the daidzein diets (1.5 ×) may be due to daidzein having the lowest anti-oxidant and phytoestergenic activity, as compared with genistein and kaempferol 33,42 . Daidzein can often produce distinctly different physiological and cell signaling responses despite a minor structural difference with genistein 44 .…”

Section: Discussionmentioning

confidence: 99%

Biopesticide synergy when combining plant flavonoids and entomopathogenic baculovirus

Hay

Behle

Berhow

et al. 2020

Sci Rep

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four crop plants known to be hosts for the lepidopteran Trichoplusia ni (soybean, green bean, cotton, and cabbage) were treated with the biopesticide AfMNPV baculovirus in a dosage response assay. Treated soybean had, on average, a 6-fold increase in virus activity compared with the other crops. Leaf trichomes on soybeans were not found to be responsible for the observed increase of insecticidal activity. Three flavonoid compounds (daidzein, genistein, and kaempferol) were uniquely found only in the soybean crop, and were not detected in cotton, cabbage, or green bean plant matter. The individual flavonoid compounds did not cause T ni. mortality in no-virus assays when incorporated into artificial insect diet. The combination of the three flavonoid compounds at leaf level concentrations significantly increased baculovirus activity in diet incorporation assays. When the daidzein, genistein, and kaempferol were added to artificial diet, at 3.5-6.5 × leaf level concentrations, virus activity increased 1.5, 2.3, and 4.2-fold for each respective flavonoid. The soybean flavonoid compounds were found to synergistically improve baculovirus activity against T. ni.Biological control has been defined as the use of predators, parasites, or pathogens to manage crop pests in a highly specific and environmentally friendly method 1 . The development of microbial-based biopesticides represents the augmentation of biological control for insect pests of plants and is increasingly viable as a commercial control tactic. The use of naturally occurring biological control agents allows growers to precisely target a pest population 2 . Research on this topic often focuses on the interaction specifically between the pathogen and target insect, such as the control efficacy provided by the application of a biopesticide. However, plants primarily protect themselves through the production of chemical defense compounds, which may alter aspects of this pest/pathogen interaction 3 . In a natural forest ecosystem, plant chemistries contribute to complex interactions depending on many parameters of the particular ecosystem, such as the density of oak trees in a forest 4 . Although comparatively less complex, reports of interactions among crop, pest, and pathogen vary for monoculture cropping systems. The susceptibility of Helicoverpa zea to Helicoverpa zea nucleopolyhedrovirus was found to be greater when fed on soybean rather than cotton, however, no specific plant chemistry was identified 5 . In okra and tomato, induced plant defense chemistries are correlated with reduced baculovirus infection of the lepidopteron Heliothis virescens (Fabricius), however induced systemic acquired resistance in cotton foliage had no effect on baculovirus infection of the lepidopteron Helicoverpa armigera (Hübner) 6,7 .A naturally occurring baculovirus was isolated and incorporated in an integrated pest management system in Brazil, which successfully controlled the soybean defoliating lepidopteran velvet bean caterpillar, Anticarsia gemmatalis Hübner 8,9 . Another...

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Genistein and daidzein treatments differently affect uterine homeostasis in the ovary-intact middle-aged rats

Cited by 15 publications

References 65 publications

Phytoestrogen genistein hinders ovarian oxidative damage and apoptotic cell death-induced by ionizing radiation: co-operative role of ER-β, TGF-β, and FOXL-2

Phytoestrogen genistein hinders ovarian oxidative damage and apoptotic cell death-induced by ionizing radiation: co-operative role of ER-β, TGF-β, and FOXL-2

Differential effects of genistein and 8-prenylgenistein on reproductive tissues in immature female mice

Biopesticide synergy when combining plant flavonoids and entomopathogenic baculovirus

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