Genistein and curcumin suppress epidermal growth factor‐induced MUC5AC mucin production and gene expression from human airway epithelial cells

Heo, Ho Jin; Lee, Su Yel; Lee, Mi Nam; Lee, Hyun Jae; Seok, Jeong Ho; Lee, Choong Jae

doi:10.1002/ptr.2801

Cited by 41 publications

(29 citation statements)

References 18 publications

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“…We have tried to investigate the possible activities of some natural products on mucin secretion from cultured airway epithelial cells. As a result of our trial, we previously reported that several natural compounds affected mucin secretion and/or production from airway epithelial cells (Lee et al, , 2004a(Lee et al, , 2004bHeo et al, 2007aHeo et al, , 2007bHeo et al, , 2009). According to traditional oriental medicine, the root of Platycodon grandiflorum has been used for regulating pulmonary inflammatory diseases (Jang, 2003).…”

Section: Introductionmentioning

confidence: 80%

Effect of Platycodin D on Airway MUC5AC Mucin Production and Gene Expression Induced by Growth Factor and Proinflammatory Factor

Lee¹,

Lee²,

Jeon³

et al. 2010

Biomolecules and Therapeutics

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-In this study, we tried to investigate whether platycodin D significantly affects MUC5AC mucin production and gene expression induced by epidermal growth factor (EGF), phorbol ester (PMA) and tumor necrosis factor-α (TNF-α) from human airway epithelial cells. Confluent NCI-H292 cells were pretreated with varying concentrations of platycodin D for 30 min and then stimulated with EGF, PMA and TNF-α for 24h, respectively. MUC5AC mucin gene expression and mucin protein production were measured by RT-PCR and ELISA. The results were as follows: (1) Platycodin D was found to inhibit the production of MUC5AC mucin protein induced by EGF, PMA, and TNF-α, respectively. (2) It also inhibited the expression of MUC5AC mucin gene induced by the same inducers. These results suggest that platycodin D can regulate mucin gene expression and production of mucin protein, by directly acting on human airway epithelial cells.

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Section: Introductionmentioning

confidence: 80%

Effect of Platycodin D on Airway MUC5AC Mucin Production and Gene Expression Induced by Growth Factor and Proinflammatory Factor

Lee¹,

Lee²,

Jeon³

et al. 2010

Biomolecules and Therapeutics

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“…We investigated the possible activities of some natural products on mucin secretion and/or production in cultured airway epithelial cells. As a result of our study, we previously reported that several natural compounds affected mucin secretion (release) and/or production from airway epithelial cells Lee et al, 2004;Heo et al, 2007;Heo et al, 2009). According to a num-www.biomolther.org berine (purity: 98.0%), were purchased from Sigma (St. Louis, MO, USA) unless otherwise specifi ed.…”

Section: Introductionmentioning

confidence: 53%

Effect of Berberine on MUC5AC Mucin Gene Expression and Mucin Production from Human Airway Epithelial Cells

Sikder¹,

Lee²,

Lee³

et al. 2011

Biomolecules and Therapeutics

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We conducted this study to investigate whether berberine signifi cantly affects MUC5AC mucin gene expression and mucin production induced by epidermal growth factor (EGF), phorbol 12-myristate 13-acetate (PMA) or tumor necrosis factor-α (TNF-α) from human airway epithelial cells. Confl uent NCI-H292 cells were pretreated with varying concentrations of berberine for 30 min and then stimulated with EGF, PMA or TNF-α for 24 h. MUC5AC mucin gene expression and mucin production were measured by reverse transcription-polymerase chain reaction (RT-PCR) and enzyme-linked immunosorbent assay (ELISA), respectively. Berberine was found to inhibit the expression of MUC5AC mucin gene induced by EGF, PMA or TNF-α. Berberine also inhibited the production of MUC5AC mucin protein stimulated by the same inducers. This result suggests that berberine can regulate the expression of mucin gene and production of mucin protein, by directly acting on human airway epithelial cells. Abstractber of reports, Coptis japonica Makino and its components, berberine, have been used to control airway allergic or infl ammatory diseases and reported to have anti-infl ammatory and anti-cancer effects (Yoo et al., 2008;Sun et al., 2009;Tang et al., 2009). On the other hand, it was previously reported that berberine stimulated basal mucin release from airway goblet cells, by our group . However, to the best of our knowledge, there are no reports about the effect of berberine on MUC5AC mucin gene expression and mucin production induced by epidermal growth factor (EGF), phorbol 12-myristate 13-acetate (PMA) or tumor necrosis factor-α (TNF-α), from human airway epithelial cells. Therefore, in this study, we investigated whether berberine affects the expression of MUC5AC mucin gene and the production of mucin protein induced by EGF, PMA or TNF-α, from the NCI-H292 human pulmonary mucoepidermoid cell line.

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“…We have tried to investigate the possible activities of some natural products on mucin secretion from cultured airway epithelial cells. As a result of our trial, we previously reported that several natural compounds affected mucin secretion and/or production from airway epithelial cells3,4,5. According to traditional oriental medicine, the root bark of Morus alba L. has been used for controlling pulmonary inflammatory diseases6 and the natural products derived it, kuwanon E, kuwanon G, mulberrofuran G, and morusin have been reported to show antibacterial, anti-inflammatory, antiviral and anticancer effects7,8,9,10.…”

Section: Introductionmentioning

confidence: 79%

Effects ofMorus albaL. and Natural Products Including Morusin onIn VivoSecretion andIn VitroProduction of Airway MUC5AC Mucin

et al. 2014

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BackgroundIt is valuable to find the potential activity of regulating the excessive mucin secretion by the compounds derived from various medicinal plants. We investigated whether aqueous extract of the root bark of Morus alba L. (AMA), kuwanon E, kuwanon G, mulberrofuran G, and morusin significantly affect the secretion and production of airway mucin using in vivo and in vitro experimental models.MethodsEffect of AMA was examined on hypersecretion of airway mucin in sulfur dioxide-induced acute bronchitis in rats. Confluent NCI-H292 cells were pretreated with ethanolic extract, kuwanon E, kuwanon G, mulberrofuran G, or morusin for 30 minutes and then stimulated with phorbol 12-myristate 13-acetate (PMA) for 24 hours. The MUC5AC mucin secretion and production were measured by enzyme-linked immunosorbent assay.ResultsAMA stimulated the secretion of airway mucin in sulfur dioxide-induced bronchitis rat model; aqueous extract, ethanolic extract, kuwanon E, kuwanon G, mulberrofuran G and morusin inhibited the production of MUC5AC mucin induced by PMA from NCI-H292 cells, respectively.ConclusionThese results suggest that extract of the root bark and the natural products derived from Morus alba L. can regulate the secretion and production of airway mucin and, at least in part, explains the folk use of extract of Morus alba L. as mucoregulators in diverse inflammatory pulmonary diseases.

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Genistein and curcumin suppress epidermal growth factor‐induced MUC5AC mucin production and gene expression from human airway epithelial cells

Cited by 41 publications

References 18 publications

Effect of Platycodin D on Airway MUC5AC Mucin Production and Gene Expression Induced by Growth Factor and Proinflammatory Factor

Effect of Platycodin D on Airway MUC5AC Mucin Production and Gene Expression Induced by Growth Factor and Proinflammatory Factor

Effect of Berberine on MUC5AC Mucin Gene Expression and Mucin Production from Human Airway Epithelial Cells

Effects ofMorus albaL. and Natural Products Including Morusin onIn VivoSecretion andIn VitroProduction of Airway MUC5AC Mucin

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