Geniposide prevents rotenone-induced apoptosis in primary cultured neurons

Li, Lin; Zhao, Juan; Liu, Ke; Li, Guanglai; Han, Yanqing; Liu, Yueze

doi:10.4103/1673-5374.167760

Cited by 11 publications

(6 citation statements)

References 28 publications

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“…GLP-1RA showed obvious neuroprotective effect in neuronal cell lines [ 39 , 40 ], animal models [ 31 , 41 ] with or without diabetes mellitus and diabetic patients [ 42 ]. In this recent study, improved viability of PC12 cells with by AGEs (600μg/ml) at 48h and HT22 cells with by AGEs (400μg/ml) at 72h by liraglutide at a concentration of 200nM or 500nM were measured ( Figures 3F , 4F ).…”

Section: Resultsmentioning

confidence: 99%

Liraglutide improved the cognitive function of diabetic mice via the receptor of advanced glycation end products down-regulation

Zhang

Chu

Zheng

et al. 2020

Aging

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Background and aims Advanced glycation end products (AGEs) and receptor of advanced glycation end products (RAGE), are associated with cognition decline. We aim to investigate the effect of liraglutide on cognitive function in diabetic mice. Results Diabetic mice showed decreased cognitive function. Moreover, lower glucagon like peptide-1 (GLP-1) levels in plasma were detected in db/db mice. Additionally, up-regulated RAGE and down-regulated glucagon like peptide-1 (GLP-1R) levels were observed in db/db mice. However, decreased GLP-1R and increased RAGE were reversed by liraglutide. We also found decreased cellular activity in cells with AGEs. Moreover, AGEs up-regulated RAGE in PC12 and HT22 cells. However, liraglutide improved the cell activity damaged by AGEs. Although we did not discover the direct-interaction between RAGE and GLP-1R, elevated RAGE levels induced by AGEs were restored by liraglutide. Conclusion We demonstrated that the cognitive function of diabetic mice was improved by liraglutide via the down-regulation of RAGE. Methods db/db mice and db/m mice were used in this study. Liraglutide was used to remedy diabetic mice. Neurons and RAGE in hippocampus were shown by immunofluorescence. And then, PC12 cells or HT22 cells with AGEs were treated with liraglutide. GLP-1R and RAGE were measured by western blotting.

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Section: Resultsmentioning

confidence: 99%

Liraglutide improved the cognitive function of diabetic mice via the receptor of advanced glycation end products down-regulation

Zhang

Chu

Zheng

et al. 2020

Aging

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“…The neurodegenerative diseases include Alzheimer’s disease (AD), Parkinson’s disease (PD), amyotrophic lateral sclerosis (ALS), frontotemporal dementia (FTD) and so on [ 1 – 4 ]. Geniposide is a pharmacologically active compound in Gardenia jasminoides Ellis (Rubiaceae) used for the treatment of hepatic disease, inflammation disorders, contusions and brain disorders [ 5 – 7 ]. Accumulated research data showed geniposide could inhibit oxidative stress and mitochondrial dysfunction [ 8 ], improve cognition [ 9 ], inhibit the interaction between amyloid-beta peptide and RAGE, attenuate Aβ-induced neuronal injury [ 10 , 11 ].…”

Section: Introductionmentioning

confidence: 99%

A mucoadhesive, thermoreversible in situ nasal gel of geniposide for neurodegenerative diseases

et al. 2017

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Neurodegenerative diseases are becoming prevalent as the population ages. Geniposide could inhibit oxidative stress, reduce apoptosis, protect neuron, and has been used for therapy of the neurodegenerative diseases. The bioavailability of geniposide by nasal route is greater than that by oral administration. However, mucociliary clearance is a rate-limiting factor for nasal route administration. The objective of this study was to develop and evaluate a mucoadhesive, thermoreversible in situ nasal gel of geniposide. The poloxamers (P407, P188) and the hydroxypropyl methylcellulose were used as thermoreversible and mucoadhesive polymers, respectively. Borneol was used as a permeation enhancer. The hydrogel was prepared with the cold method and optimized by the response surface methodology-central composite design. Gelation temperature, pH, clarity, gel strength, mucoadhesive strength, in vitro and ex vivo release kinetics of formulations were evaluated. The optimized amounts of poloxamer407 (P407), poloxamer188 (P188) and hydroxypropyl methylcellulose were determined to be 19.4–20.5%, 1.1–4.0% and 0.3–0.6% respectively. The second-order polynomial equation in terms of actual factors indicated a satisfactory correlation between the independent variables and the response (R2 = 0.9760). An ANOVA of the empirical second-order polynomial model indicated the model was significant (P<0.01). P407, P188, P407×P188, P4072 and P1882 were significant model terms. The effects of P407 on gelation temperature were greater than those of other independent variables. The pH values of all the formulations were found to be within 6.3–6.5 which was in the nasal physiological pH range 4.5–6.5. The drug content, gel strength, mucoadhesive strength of the optimized formulations were 97–101%, 25–50 sec and 4000–6000 dyn/cm2 respectively. The in vitro release kinetics of cumulative release of geniposide was fitted to the zero-order model. The ex vivo cumulative release kinetics of geniposide was fitted to the Weibull model. This study concludes that the release of geniposide is controlled by gel corrosion, and that the permeation of geniposide is time-dependent. The more residence time, mucoadhesive, thermoreversible in situ nasal gel of geniposide for neurodegenerative diseases is of compliance and potential application.

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“…At the cellular level, GP antagonized Aβ1-42-induced neurotoxicity by inhibiting the Akt/mTOR signalling pathway and enhancing autophagy in an Aβ1-42-induced in vitro model of AD in the human neuroblastoma SH-SY5Y cell line [ 43 ]. GP attenuates rotenone-induced primary neuronal injury by inhibiting apoptosis [ 44 ]. GP significantly reduced the level of tau phosphorylation and accelerated the phosphorylation of GSK-3β in the brains of APP/PS1 transgenic mice with insulin deficiency [ 44 ].…”

Section: Discussionmentioning

confidence: 99%

A network pharmacology approach to reveal the key ingredients in Scrophulariae Radix (SR) and their effects against Alzheimer's disease

Shi,

Chen,

Huang

et al. 2024

Heliyon

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Geniposide prevents rotenone-induced apoptosis in primary cultured neurons

Cited by 11 publications

References 28 publications

Liraglutide improved the cognitive function of diabetic mice via the receptor of advanced glycation end products down-regulation

Liraglutide improved the cognitive function of diabetic mice via the receptor of advanced glycation end products down-regulation

A mucoadhesive, thermoreversible in situ nasal gel of geniposide for neurodegenerative diseases

A network pharmacology approach to reveal the key ingredients in Scrophulariae Radix (SR) and their effects against Alzheimer's disease

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