Genipin-crosslinked electrospun chitosan nanofibers: Determination of crosslinking conditions and evaluation of cytocompatibility

Q, Li; Wang, Xianliu; Lou, Xiangxin; Yuan, Huihua; Tu, Hongbin; Li, Biyun; Zhang, Yanzhong

doi:10.1016/j.carbpol.2015.05.039

Cited by 83 publications

(40 citation statements)

References 41 publications

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“…Biological studies with chitosan cross-linked with GTA have demonstrated cytotoxicity. On the contrary, GNP crosslinked chitosan materials have showed good biocompatibility towards human RPE cells and low acute toxicity in vivo [21,29], making them more suitable for regeneration of tissues [29][30][31][32][33]. Meanwhile, GTA cross-linked chitosan fibers triggered apoptosis at earlier stage on cells.…”

Section: Introductionmentioning

confidence: 99%

Comparative Study of One-Step Cross-Linked Electrospun Chitosan-Based Membranes

Aguirre-Chagala

Pavón-Pérez

Altúzar

et al. 2017

Journal of Nanomaterials

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Chitosan membranes are widely applied for tissue engineering; however, a major drawback is their low resistance in aqueous phases and therefore the structure collapses impeding their long-term use. Although there is extensive research, because of chitosan’s importance as a biomaterial, studies involving chitosan-based membranes are still needed. Herein, a detailed investigation of diverse chemical routes to cross-link fibers in situ by electrospinning process is described. In case of using genipin as cross-linker, a close relationship with the content and the mean diameter values is reported, suggesting a crucial effect over the design of nanostructures. Also, the physical resistance is enhanced for the combination of two types of methods, such as chemical and physical methods. Cross-linked fibers upon exposure to long wave ultraviolet A (UVA light) change their morphology, but not their chemical composition. When they are incubated in aqueous phase for 70 days, they show an extensive improvement of their macrostructural integrity which makes them attractive candidates for tissue engineering application. As a result, the thermal properties of these materials reveal less crystallinity and higher temperature of degradation.

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Section: Introductionmentioning

confidence: 99%

Comparative Study of One-Step Cross-Linked Electrospun Chitosan-Based Membranes

Aguirre-Chagala

Pavón-Pérez

Altúzar

et al. 2017

Journal of Nanomaterials

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“…Loss of fibrous structure upon contact with aqueous solutions could limit practical utilization of 3D-plotted or electrospun chitosan nanofibers. To meet the demands for tissue engineering uses [ 112 ], post-electrospinning crosslinking may be performed to inhibit solubility and improve mechanical properties [ 113 ].…”

Section: Bone Regenerationmentioning

confidence: 99%

Genipin-Crosslinked Chitosan Gels and Scaffolds for Tissue Engineering and Regeneration of Cartilage and Bone

et al. 2015

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The present review article intends to direct attention to the technological advances made since 2009 in the area of genipin-crosslinked chitosan (GEN-chitosan) hydrogels. After a concise introduction on the well recognized characteristics of medical grade chitosan and food grade genipin, the properties of GEN-chitosan obtained with a safe, spontaneous and irreversible chemical reaction, and the quality assessment of the gels are reviewed. The antibacterial activity of GEN-chitosan has been well assessed in the treatment of gastric infections supported by Helicobacter pylori. Therapies based on chitosan alginate crosslinked with genipin include stem cell transplantation, and development of contraction free biomaterials suitable for cartilage engineering. Collagen, gelatin and other proteins have been associated to said hydrogels in view of the regeneration of the cartilage. Viability and proliferation of fibroblasts were impressively enhanced upon addition of poly-l-lysine. The modulation of the osteocytes has been achieved in various ways by applying advanced technologies such as 3D-plotting and electrospinning of biomimetic scaffolds, with optional addition of nano hydroxyapatite to the formulations. A wealth of biotechnological advances and know-how has permitted reaching outstanding results in crucial areas such as cranio-facial surgery, orthopedics and dentistry. It is mandatory to use scaffolds fully characterized in terms of porosity, pore size, swelling, wettability, compressive strength, and degree of acetylation, if the osteogenic differentiation of human mesenchymal stem cells is sought: in fact, the novel characteristics imparted by GEN-chitosan must be simultaneously of physico-chemical and cytological nature. Owing to their high standard, the scientific publications dated 2010–2015 have met the expectations of an interdisciplinary audience.

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“…The most exploited sources of chitin are the shells of crabs and shrimps. Because of its non‐toxicity, stability, biodegradability, and similarity to the internal tissues of the body, chitosan has found itself as a very versatile material with extensive application in the biomedical and biotechnological fields . The swelling properties of chitosan have been controlled by crosslinking using crosslinking agents such as glutaldehyde, and genipin .…”

Section: Introductionmentioning

confidence: 99%

pH‐Responsive Studies of Bacterial Cellulose /Chitosan Hydrogels Crosslinked with Genipin: Swelling and Drug Release Behaviour

et al. 2019

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Semi interpenetrating hydrogels (semi-IPN) of bacterial cellulose (BC) and chitosan (Ch) crosslinked with genipin were prepared and characterised using Differential Scanning Calorimetry (DSC), Fourier Transform Infrared Spectroscopy (FTIR) and Scanning Electron Microscopy (SEM). Infrared spectra showed amide I and II absorption peaks at 1632 and 1554 cm À 1 and CÀ N stretching absorptions at 1250 and 1020 cm À 1 , respectively, for crosslinked hydrogels. The fibrous structure of BC and the porosity in the hydrogels were revealed by SEM. Swelling of hydrogels was sensitive to pH, and maximum at pH 1.0. Swelling of non-crosslinked samples decreased as the pH increased. However, with crosslinked hydrogels, swelling increased as chitosan ratio increased at low pH, as well as with increase in BC ratio at high pH. Free, bound and intermediate water types in hydrogels were revealed by DSC. The release kinetics of Quetiapine fumarate (QF), an antipsychotic drug used for the treatment of Schizophrenia was studied using UV spectrometry, followed predominantly, the Higuchi model at all pH for crosslinked hydrogels. The transport mechanism of hydrogels was a combination of non-Fickian and Super Case II. Crosslinked hydrogels showed controlled drug release behaviour. These hydrogel systems possess potential application in pharmaceutical field as all chemicals used in the development of the hydrogels are non-toxic, with BCÀ Ch 60:40 showing most promising potential.[a] J.

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Genipin-crosslinked electrospun chitosan nanofibers: Determination of crosslinking conditions and evaluation of cytocompatibility

Cited by 83 publications

References 41 publications

Comparative Study of One-Step Cross-Linked Electrospun Chitosan-Based Membranes

Comparative Study of One-Step Cross-Linked Electrospun Chitosan-Based Membranes

Genipin-Crosslinked Chitosan Gels and Scaffolds for Tissue Engineering and Regeneration of Cartilage and Bone

pH‐Responsive Studies of Bacterial Cellulose /Chitosan Hydrogels Crosslinked with Genipin: Swelling and Drug Release Behaviour

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