Genipin cross-linked decellularized tracheal tubular matrix for tracheal tissue engineering applications

Sun, Fei; Yuan, Jun; Xu, Yan; Shi, Hongcan; Zhang, Siquan; Liu, Xingchen; Pan, Shu; Ye, Gang; Zhang, Weidong; Zhang, Fangbiao; Zhong, Chonghao

doi:10.1038/srep24429

Cited by 36 publications

(29 citation statements)

References 39 publications

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“…A similar pattern was observed in the scanning electron microscopy images with an increase in the compactness of fibers at higher formaldehyde concentrations ( Figure 3a-d), can be considered as the effect of the cross-linking reaction. Similar results have been reported by the treatment with crosslinking agents, such as glutaraldehyde and genipin (Sun et al, 2016).…”

Section: Discussionsupporting

confidence: 89%

Controlled cross‐linking of porcine cholecyst extracellular matrix for preparing tissue engineering scaffold

Mony

Anilkumar

2019

J Biomed Mater Res

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Treatment with cross‐linking agents for stabilizing biomolecules is an integral step during the preparation of many extracellular matrix‐based tissue engineering scaffolds from mammalian organs. However, excess cross‐linking may cause nonavailability of biomolecules and consequent deterioration of bioinductive properties of the scaffold. The present study considered controlling the extent of cross‐linking in a porcine cholecyst extracellular matrix scaffold prepared by a nonenzymatic and nondetergent method, by ex situ incubation of the source organ in varying concentrations of neutral buffered formaldehyde (10, 4, 1 or 0%; v/v) for in situ cross‐linking of biomolecules. Reduction of the formaldehyde concentration resulted in an increase in the extent of biodegradation and a decrease in the compactness of the mesh‐like surface microarchitecture of the scaffold. Retention of collagen was maximum when treated with 10% neutral buffered formaldehyde without any variation in the content of elastin and sulphated glycosaminoglycans. Although there was a reduction in the quantity of growth factors following the cross‐linking, fibroblasts remained viable on the scaffolds. The retention of major biomolecule was maximum and autodigestion was minimum in the scaffold prepared by the ex situ treatment of cholecyst in 10% neutral buffered formalin and found suitable for preparing the tissue engineering scaffold.

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Section: Discussionsupporting

confidence: 89%

Controlled cross‐linking of porcine cholecyst extracellular matrix for preparing tissue engineering scaffold

Mony

Anilkumar

2019

J Biomed Mater Res

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“…Subcutaneous in vivo implanted slices from different crosslinking groups showed increased resistance against degradation. This finding might indicate a masking effect of crosslinking on the cleavage sites of collagen that prevents enzymes penetrating into the treated scaffolds [36]. Qualitatively, there were no differences in the sizes of the implanted slices among the different cross-linking groups after 21 days of implantation.…”

Section: Discussionmentioning

confidence: 82%

Silver nanoparticles improve structural stability and biocompatibility of decellularized porcine liver

Saleh

Ahmed

et al. 2018

Artificial Cells, Nanomedicine, and Biotechnology

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No ideal cross-linking agent has been identified for decellularized livers (DLs) yet. In this study, we evaluated structural improvements and biocompatibility of porcine DLs after cross-linking with silver nanoparticles (AgNPs). Porcine liver slices were decellularized and then loaded with AgNPs (100 nm) after optimization of the highest non-toxic concentration (5 µg/mL) using Human hepatocellular carcinoma (HepG2) and EAhy926 human endothelial cell lines. The cross-linking effect of AgNPs was evaluated and compared to that of glutaraldehyde and ethyl carbodiimide hydrochloride and N-hydroxysuccinimide. The results indicated that AgNPs improved the ultra-structure of DLs' collagen fibres with good porosity and increased DLs' resistance against in vitro degradation with good cytocompatibility. AgNPs decreased the host inflammatory reaction against implanted porcine DL slices in vivo and increased the polarization of M2 macrophages. Thus, structural and functional improvements of Porcine DLs could be achieved using AgNPs.

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“…7 This process removes the cellular components that cause tissue rejection and preserves the three-dimensional structure of the trachea and the extracellular matrix (ECM) components, providing a material scaffold for cell growth and proliferation. 10–13 Currently, decellularized tracheal scaffolds are often prepared by the detergent-enzymatic method (DEM), which removes the tracheal antigens while retaining the three-dimensional structure and ECM of the trachea, providing a compatible and supportive environment for the graft. 14–17 There are clinical cases of transplanted acellular tracheal stroma, 10,18 some of which have been relatively successful, but the function of these grafts needs to be improved.…”

Section: Introductionmentioning

confidence: 99%

Rapid preparation of decellularized trachea as a 3D scaffold for organ engineering

et al. 2020

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Objective: To shorten the preparation time of rabbit decellularized tracheal matrix through a modified detergent-enzymatic method with higher concentration of DNase (50 kU/mL), providing an experimental and theoretical basis for clinical decellularization technology. Methods: The control group was a natural trachea, and the experimental group was a tracheal matrix subjected to two and four decellularization cycles. The performance of each group of samples was evaluated by histology and immunohistochemical staining, scanning electron microscopy, biomechanical property testing, inoculation and cytotoxicity tests, and allograft experiments. Results: The results showed that the nuclei of the nonchondral areas of the tracheal stroma were essentially completely removed and MHC-I and MHC-II antigens were removed after two decellularization cycles. Histological staining and scanning electron microscopy showed that the extracellular matrix was retained and the basement membrane was intact. Cell inoculation and proliferation tests confirmed that the acellular tracheal matrix had good biocompatibility, and the proliferation capacity of bone mesenchymal stem cells on the matrix was increased in the experimental group compared with the control group ( p < 0.05). Histological staining and CD68 molecular marker analysis after the allograft experiment showed that the inflammatory response of the acellular tracheal matrix was weak and the infiltration of surrounding macrophages was reduced. Conclusion: A modified detergent-enzymatic method with an increased DNase (50 kU/mL) concentration requires only two cycles (4 days) to obtain a decellularized rabbit tracheal matrix with a short preparation time, good biocompatibility, suitable mechanical properties, and reduced preparation cost.

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Genipin cross-linked decellularized tracheal tubular matrix for tracheal tissue engineering applications

Cited by 36 publications

References 39 publications

Controlled cross‐linking of porcine cholecyst extracellular matrix for preparing tissue engineering scaffold

Controlled cross‐linking of porcine cholecyst extracellular matrix for preparing tissue engineering scaffold

Silver nanoparticles improve structural stability and biocompatibility of decellularized porcine liver

Rapid preparation of decellularized trachea as a 3D scaffold for organ engineering

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