Genic Variability in the Longnose Dace, Rhinichthys cataractae

Merritt, Robert B.; Rogers, Jean F.; Kurz, Brenda

doi:10.2307/2407414

Cited by 16 publications

(10 citation statements)

References 18 publications

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“…It has been suggested, however, that selection acting at several loci in some concerted fashion may be more effective than selection acting at a single locus (Sing & Templeton, 1975;Mitton & Koehn, 1976;Merritt et al, 1978). Kirpichnikov & Muske (1980) reported that in sockeye salmon, Oncorhynchus nerka, several polymorphic loci show latitudinal clines in allele distribution.…”

Section: Discussionmentioning

confidence: 99%

Correlations of allele frequencies with physical and environmental variables for populations of largemouth bass, Micropterus salmoides (Lacepede)

Philipp

Childers

Whitt

1985

Journal of Fish Biology

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Differences in the biochemical genetic structure of each of 90 populations of largemouth bass, Microprerus salmoides (Lacepede), were detected by vertical starch gel electrophoresis. The allele frequencies at each of 28 enzyme loci were tested for correlation with 24 physical and environmental variables determined for each body of water from which the populations were sampled. The allele frequencies at several loci (malate dehydrogenase-B, ismitrate dehydrogenase-B, superoxide dismutase-A, and aspartate aminotransferase-B) were highly significantly correlated (PSO.0001) with several physical and environmental variables (latitude. heating degree-days, cooling degree-days, and the length of the growing season). These correlations are consistent with the hypothesis that allelic differences at specific enzyme loci can confer different degrees of fitness in different thermal environments. Additional laboratory and field studies will be required to determine the extent to which allelic variants at a given enzyme locus contribute to thermal tolerance and thermal preference of largemouth bass.

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Section: Discussionmentioning

confidence: 99%

Correlations of allele frequencies with physical and environmental variables for populations of largemouth bass, Micropterus salmoides (Lacepede)

Philipp

Childers

Whitt

1985

Journal of Fish Biology

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“…Peptidases were visualized using the histochemical staining procedures modified from Merritt et al (1978). For each slice of the gel, 0.04 g of peptide substrate, 0.01 g of snake venom L-amino acid oxidase (from either Crotalus atrox or C. adamanteus), 0.02 g of horseradish peroxidase, and 0.01 g of O-dianisidine were mixed in approximately 15 ml of 0.2 M Tris-HC1 (pH 8.0) buffer.…”

Section: Methodsmentioning

confidence: 99%

Avian peptidase isozymes: Tissue distributions, substrate affinities, and assignment of homology

Matson

1989

Biochem Genet

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Peptidase (EC 3.4.13.9 and EC 3.4.13.11) isozymes were examined from 14 tissues of 19 species of birds, representing 8 families and 6 orders, via horizontal starch gel electrophoresis. Peptidases were detected by employing histochemical staining techniques utilizing 13 di-, tri-, and tetrapeptides as substrates. It was determined that there is a minimum of six presumptive structural gene loci encoding peptidases in birds (excluding those encoding cytosol aminopeptidase; EC 3.4.11.1). The products of the peptidase loci were widely expressed in most tissues with the exception of Pep-F, which was restricted to pancreas. Products of most loci have multiple, overlapping substrate affinities (except for the products of Pep-D, which react only with a peptide containing a carboxyterminal proline). By using a combination of differential electrophoretic mobility and substrate preferences, the products of the various peptidase loci can be distinguished from one another. The homologies of the avian peptidases with those in other vertebrates were tentatively established and it is suggested that the nomenclature proposed by Rapley et al.

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“…Nevertheless, the species-protein interaction remained overwhelming (67 per cent of total variance). To investigate the effect of study bias on the drainage-protein interaction, we identified seven studies (39,8,13,31,22,24,33; see Appendix) in each of which a single species was scored from several drainage basins. Only two variance components were substantial.…”

Section: _jmentioning

confidence: 99%

“…The main effects of protein (4 per cent of total variance) and species (9 per cent) remained small and non-significant, while the species-protein interaction contributed 80 per cent of the variance and was significant at P<0.0005. For mammals, we used the eight proteins most often reported (AAT, ALB, IDH, LDH, MDH, PGDH, PGM, TF; see Table 2), for five populations of each of six species (28,29,31,32,34,39; see Table 1). The main effects of species and protein were negligible (1 per cent each) and non-significant, while the interaction remained large (39 per cent) and highly significant (P< 0.0005).…”

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confidence: 99%

Sources of variance in protein heterozygosity: the importance of the species-protein interaction

1992

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We report on a detailed survey of protein heterozygosity in Canadian freshwater fish and mammals.A simple one-way analysis showed substantial variance among species. However, a two-way analysis of species and proteins showed that there was little if any variance among species or among proteins, but a very large species-protein interaction. We could not remove this interaction by analysing taxa separately, by constructing completely balanced datasets, by eliminating study bias or by excluding monomorphic proteins, and we could not decompose the interaction by classifying enzymes according to their form and function. We conclude that most of the variance in protein heterozygosity is attributable to species-protein interaction. This casts some doubt on the interpretation of comparative studies of mean heterozygosity among species or among proteins. Our result seems inconsistent with the neutral theory of protein variation but not with the differential action of natural selection.

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Genic Variability in the Longnose Dace, Rhinichthys cataractae

Cited by 16 publications

References 18 publications

Correlations of allele frequencies with physical and environmental variables for populations of largemouth bass, Micropterus salmoides (Lacepede)

Correlations of allele frequencies with physical and environmental variables for populations of largemouth bass, Micropterus salmoides (Lacepede)

Avian peptidase isozymes: Tissue distributions, substrate affinities, and assignment of homology

Sources of variance in protein heterozygosity: the importance of the species-protein interaction

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