Genetics on a maize cob: A teaching tool for schools

Hossain, Firoz; Bhat, S. R.; Mohapatra, Trilochan; Singh, Ashok Kumar

doi:10.31742/ijgpb.79s.1.27

Cited by 6 publications

(5 citation statements)

References 43 publications

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“…To be able to solve all these problems, a cereal species is the most viable option, as the dry ears can be stored and maintained for successive student generations, allowing for phenotypic studies without the need to cultivate the lines yearly. In this sense, some interesting resources have been developed in maize for teaching purposes [ 10 ].…”

Section: Introductionmentioning

confidence: 99%

An F2 Barley Population as a Tool for Teaching Mendelian Genetics

Giménez

Benavente

Pascual

et al. 2021

Plants

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In the context of a general genetics course, mathematical descriptions of Mendelian inheritance and population genetics are sometimes discouraging and students often have serious misconceptions. Innovative strategies in expositive classes can clearly encourage student’s motivation and participation, but laboratories and practical classes are generally the students’ favourite academic activities. The design of lab practices focused on learning abstract concepts such as genetic interaction, genetic linkage, genetic recombination, gene mapping, or molecular markers is a complex task that requires suitable segregant materials. The optimal population for pedagogical purposes is an F2 population, which is extremely useful not only in explaining different key concepts of genetics (as dominance, epistasis, and linkage) but also in introducing additional curricular tools, particularly concerning statistical analysis. Among various model organisms available, barley possesses several unique features for demonstrating genetic principles. Therefore, we generated a barley F2 population from the parental lines of the Oregon Wolfe Barley collection. The objective of this work is to present this F2 population as a model to teach Mendelian genetics in a medium–high-level genetics course. We provide an exhaustive phenotypic and genotypic description of this plant material that, together with a description of the specific methodologies and practical exercises, can be helpful for transferring our fruitful experience to anyone interested in implementing this educational resource in his/her teaching.

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Section: Introductionmentioning

confidence: 99%

An F2 Barley Population as a Tool for Teaching Mendelian Genetics

Giménez

Benavente

Pascual

et al. 2021

Plants

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“…However, the recessive y1 allele is unable to catalyse the reaction and makes the kernel devoid of any carotenoids and eventually kernels look white 34 . In BC 2 F 3 seeds borne on BC 2 F 2 ears, Y1 gene segregated in four forms viz., (i) dark yellow (Y1Y1Y1), medium yellow (Y1Y1y1), light yellow (Y1y1y1) and white (y1y1y1) in the endosperm 54 . We selected only the white kernels to raise the BC 2 F 3 progenies, and eventually develop white grained waxy hybrids.…”

Section: Discussionmentioning

confidence: 99%

Combining higher accumulation of amylopectin, lysine and tryptophan in maize hybrids through genomics-assisted stacking of waxy1 and opaque2 genes

Talukder

Muthusamy

Chhabra

et al. 2022

Sci Rep

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Waxy maize rich in amylopectin has emerged as a preferred food. However, waxy maize is poor in lysine and tryptophan, deficiency of which cause severe health problems. So far, no waxy hybrid with high lysine and tryptophan has been developed and commercialized. Here, we combined recessive waxy1 (wx1) and opaque2 (o2) genes in the parental lines of four popular hybrids (HQPM1, HQPM4, HQPM5, and HQPM7) using genomics-assisted breeding. The gene-based markers, wx-2507F/RG and phi057 specific for wx1 and o2, respectively were successfully used to genotype BC1F1, BC2F1 and BC2F2 populations. Background selection with > 100 SSRs resulted in recovering > 94% of the recurrent parent genome. The reconstituted hybrids showed 1.4-fold increase in amylopectin (mean: 98.84%) compared to the original hybrids (mean: 72.45%). The reconstituted hybrids also showed 14.3% and 14.6% increase in lysine (mean: 0.384%) and tryptophan (mean: 0.102%), respectively over the original hybrids (lysine: 0.336%, tryptophan: 0.089%). Reconstituted hybrids also possessed similar grain yield (mean: 6248 kg/ha) with their original versions (mean: 6111 kg/ha). The waxy hybrids with high lysine and tryptophan assume great significance in alleviating malnutrition through sustainable and cost-effective means. This is the first report of development of lysine and tryptophan rich waxy hybrids using genomics-assisted selection.

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“…Purple corn has more anthocyanin content than other varieties of corn (Georgiana et al 2019). This variety can be produced not only by crossing purple corn with other purple corn but also by crossing with non-purple corn since the dominant behavior of the P1 gene can generate a hybrid of purple corn (Hossain et al 2019). One initiative to increase corn quality is to develop a new corn cultivar by incorporating kernel color characteristics and combining it with other specialty corn such as waxy and sweet corn.…”

Section: Introductionmentioning

confidence: 99%

Assessment of combining ability in purple corn parents under line × tester mating design using GGE biplot

2021

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Abstract. Mufidah N, Sugiharto AN, Waluyo B. 2021. Assessment of combining ability in purple corn parents under line × tester mating design using GGE biplot. Biodiversitas 22: 4545-4554. One strategy to increase corn quality is the development of new corn cultivars by incorporating the character of nutritional content. The study aims to obtain information on the combining ability of purple corn lines as well as determine the best parental lines for purple corn hybrid development using the GGE biplot approach. This study used 24 hybrids generated from crossings of 8 purple corn lines with 3 testers, which were crossed using a line × tester mating design. Plant performance was assessed using a randomized block design of three replications. The F-test was used to assess and test the experiment variance. The combining ability of purple corn lines was examined using the GGE biplot method. The result showed that the GGE biplot approach successfully identified the best combiner and combinations generated between purple corn lines and non-purple corn lines. Purple corn lines P1Y1 had the best general combining ability for fresh ear yield and total anthocyanin content, meanwhile, P1Y2 had the best general combining ability in most of the agronomic traits, namely ear diameter, shelled ear weight, number of grains row-1, and as well as total anthocyanin content. These 2 lines were also identified as the best parent chosen in the best combination in this study.

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Genetics on a maize cob: A teaching tool for schools

Cited by 6 publications

References 43 publications

An F2 Barley Population as a Tool for Teaching Mendelian Genetics

An F2 Barley Population as a Tool for Teaching Mendelian Genetics

Combining higher accumulation of amylopectin, lysine and tryptophan in maize hybrids through genomics-assisted stacking of waxy1 and opaque2 genes

Assessment of combining ability in purple corn parents under line × tester mating design using GGE biplot

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