“…More recent studies by Holmes and co-workers revealed that some E. coli strains produce enterotoxins (LT-IIa, LT-IIb) that are antigenically distinct and differ significantly in amino acid sequences from LT-I or CT, although they also cause elevation of intracellular cyclic AMP (17,19,21,22). Based on comparison of toxin nucleotide sequences, it was hypothesized that the A subunits of all toxins in the LT family originated from a common ancestral gene, with separate branches leading to the LT-I and LT-II serogroups (21,22 amino acid sequences of toxin Al, A2, and B polypeptides revealed that Al, in particular the amino terminus of Al, is conserved to the greatest extent (21,22).…”