Genetics of Thermophiles

A new metabolic pathway for <i>sym</i>-homospermidine synthesis in an extreme thermophile, <i>Thermus thermophilus.</i>

“…The genes used in the present study are speB (coding for aminopropylagmatinase, TTHA 1129) from genome of strain HB8 (Yokoyama et al, 2000), and TTC0764 from genome of strain HB27 (Henne et al, 2004)), speD2(coding for S-adenosylmethionine decarboxylase, TTHA1457, TTC1093), speE (coding for agmatine/triamine aminopropyltransferase, TTHA0824, TTC0472), and dhs (annotated to be deoxyhypusine synthase, TTHA1570, TTC1205). Mutants are constructed as reported in literatures Ohnuma et al, 2005;Oshima and Tamakoshi, 2011) using standard techniques. T. thermophilus knockout mutants, ΔspeE, ΔspeD2, and ΔspeB, were constructed by inserting kanamycin resistance gene (htk).…”

“…The chromosomal DNA and plasmid DNA were prepared and purified using Quiagen kits. For polyamine analysis, wild type strains and mutants were grown in a synthetic medium (Oshima and Tamakoshi, 2011;Tanaka et al, 1981;Takada et al, 1993).…”