Genetics of soybean seed lipoxygenases and linolenic acid content in seeds of the soybean wild ancestor

Lenis, Julián M.

doi:10.32469/10355/14213

Cited by 4 publications

(5 citation statements)

References 85 publications

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“…While investigating the inheritance of the α-linolenic acid trait, they observed that separate alleles from each parent recombined in an additive manner to increase the α-linolenic acid concentration in the progenies from an interspecific cross between G. max and G. soja. Another study suggested complex inheritance of the high α-linolenic acid trait from a wild soybean accession independent of the FAD3 alleles [30].…”

Section: Resultsmentioning

confidence: 99%

Genetic Improvement of the Fatty Acid Biosynthesis System to Alter the ω‐6/ω‐3 Ratio in the Soybean Seed

Kulkarni

Kim

Song

et al. 2017

J Americ Oil Chem Soc

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A high ω‐6/ω‐3 fatty acid ratio in the soybean seed adversely affects human health. The objective of the present study was to improve the fatty acid biosynthesis to reduce the ω‐6/ω‐3 ratio by combining the FAD2‐1A and FAD2‐1B mutant alleles with α‐linolenic acid (ω‐3) related alleles from wild soybean. The F2 population comprising 2320 F2:3 lines developed from S08‐14717 × PI 483463 cross exhibited significant variation for fatty acid components. Of these, 114 lines were advanced to the F5:6 generation and genotyped for FAD2‐1A and FAD2‐1B alleles. The lines carrying mutant FAD2‐1A and FAD2‐1B alleles showed ~ 761 g kg−1 oleic, and ~ 50 g kg−1 linoleic acids, which reduced ω‐6/ω‐3 ratios to ~ 0.6. Conversely, the lines carrying FAD2‐1A or FAD2‐1B mutant alleles had 267 or 399 g kg−1 oleic, 327 or 471 g kg−1 linoleic, and 120 or 130 g kg−1 α‐linolenic acids concentration, respectively. The elevated α‐linolenic acid resulted in the reduction of ω‐6/ω‐3 ratios in the range 2.5–3.9. The present study demonstrated that combining FAD2 mutant alleles with α‐linolenic acid‐related alleles from wild soybean reduces the seed ω‐6/ω‐3 ratio.

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Section: Resultsmentioning

confidence: 99%

Genetic Improvement of the Fatty Acid Biosynthesis System to Alter the ω‐6/ω‐3 Ratio in the Soybean Seed

Kulkarni

Kim

Song

et al. 2017

J Americ Oil Chem Soc

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“…The interspecific mapping population used in this study was first genotyped using the GoldenGate ® assay, which contained 1536 SNP loci with 169 F 4:5 RILs derived from 'Williams 82' × 'PI366121' [41]. The genetic map was constructed with 414 polymorphic and filtered SNPs to detect the QTL regions for foxglove aphid resistance [42,43], 100-seed weight [44], and seed starch content [40].…”

Section: Discussionmentioning

confidence: 99%

Identification of Quantitative Trait Loci Controlling Root Morphological Traits in an Interspecific Soybean Population Using 2D Imagery Data

Islam,

Ghimire,

Lay

et al. 2024

IJMS

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Roots are the hidden and most important part of plants. They serve as stabilizers and channels for uptaking water and nutrients and play a crucial role in the growth and development of plants. Here, two-dimensional image data were used to identify quantitative trait loci (QTL) controlling root traits in an interspecific mapping population derived from a cross between wild soybean ‘PI366121’ and cultivar ‘Williams 82’. A total of 2830 single-nucleotide polymorphisms were used for genotyping, constructing genetic linkage maps, and analyzing QTLs. Forty-two QTLs were identified on twelve chromosomes, twelve of which were identified as major QTLs, with a phenotypic variation range of 36.12% to 39.11% and a logarithm of odds value range of 12.01 to 17.35. Two significant QTL regions for the average diameter, root volume, and link average diameter root traits were detected on chromosomes 3 and 13, and both wild and cultivated soybeans contributed positive alleles. Six candidate genes, Glyma.03G027500 (transketolase/glycoaldehyde transferase), Glyma.03G014500 (dehydrogenases), Glyma.13G341500 (leucine-rich repeat receptor-like protein kinase), Glyma.13G341400 (AGC kinase family protein), Glyma.13G331900 (60S ribosomal protein), and Glyma.13G333100 (aquaporin transporter) showed higher expression in root tissues based on publicly available transcriptome data. These results will help breeders improve soybean genetic components and enhance soybean root morphological traits using desirable alleles from wild soybeans.

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“…DNA extraction and linkage mapping: Genomic DNA was isolated from the young trifoliate leaves from individual plants following the modified CTAB (hexadecyltrimethylammonium bromide) method (Lenis 2011). Earlier, 169 RILs from the cross 'Williams 82' and 'PI 366121' were genotyped with 1536 SNP USLP 1.0 panel using the GoldenGate assay (Lee et al 2015).…”

Section: Methodsmentioning

confidence: 99%

Mapping quantitative trait loci controlling soybean seed starch content in an interspecific cross of ‘Williams 82’ (Glycine max) and ‘PI 366121’ (Glycine soja)

et al. 2017

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Seed starch content (SSC) greatly affects the taste, flavour and processing properties of soy foods. The objective in this study was to identify quantitative trait loci (QTL) for SSC in soybean. A total in 169 recombinant inbred lines (RILs) derived from a cross in ‘Williams 82’ and ‘PI 366121’ were grown for three consecutive years. The SSC of the RILs displayed continuous variation with transgressive segregation and hence amenable for QTL mapping. Nine significant QTL exhibiting 5.6–11.3% of the total phenotypic variation (PVE) were identified. The QTL qSTR06_2 showed highest PVE (9.1–11.3%) at LOD values of 4.25–5.39. No stable QTL over 3 years were identified, indicating strong environmental influence on SSC. The QTL qSTR11_1 and qSTR20_1 were found to colocalize with some of the previously reported QTL for sucrose content in soybean, implying the interrelationship between starch and sucrose biosynthesis. As the carbohydrate components may affect key constituents such as oil and protein in soybean seed, findings of the study may be useful in breeding soybeans with improved seed composition.

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Genetics of soybean seed lipoxygenases and linolenic acid content in seeds of the soybean wild ancestor

Cited by 4 publications

References 85 publications

Genetic Improvement of the Fatty Acid Biosynthesis System to Alter the ω‐6/ω‐3 Ratio in the Soybean Seed

Genetic Improvement of the Fatty Acid Biosynthesis System to Alter the ω‐6/ω‐3 Ratio in the Soybean Seed

Identification of Quantitative Trait Loci Controlling Root Morphological Traits in an Interspecific Soybean Population Using 2D Imagery Data

Mapping quantitative trait loci controlling soybean seed starch content in an interspecific cross of ‘Williams 82’ (Glycine max) and ‘PI 366121’ (Glycine soja)

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