Genetics of Skin Disease in Horses

Lindgren, Gabriella; Naboulsi, Rakan; Frey, Rebecka; Solé, Marina

doi:10.1016/j.cveq.2020.03.010

Cited by 6 publications

(5 citation statements)

References 74 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

Section: Resultsmentioning

confidence: 99%

“…The equine dermis has a heterogeneous cell population of which fibroblasts are the primary cells supported by skin dendrocytes, principal elements of the equine cutaneous immune system. 31 The dermis is subdivided into the superficial papillary and deep reticular dermis. In the upper regions of the superficial papillary dermis, just below the epidermis, CBR1 (Figure 2C1 ‐C4) and CBR2 (Figure 3C1 ‐C4) expression was seen in fibroblast‐like cells, and their expression levels decreased in the deeper dermis parts.…”

Section: Resultsmentioning

confidence: 99%

“…The equine dermis has a heterogeneous cell population of which fibroblasts are the primary cells supported by skin dendrocytes, principal elements of the equine cutaneous immune system. 31 The dermis is subdivided into the superficial papillary and deep reticular dermis. In However, although rabbit polyclonal antibodies for CBRs, applied during confocal microscopy, allowed us to demonstrate CBRs distribution in the tissues, verification to assess their specificity, as well as quantification of the CBRs, was performed using a WB method in the whole skin tissue and brain samples (Figure 4).…”

Section: Dermal Expression Of Cbrsmentioning

confidence: 99%

See 2 more Smart Citations

The cannabinoid receptors system in horses: Tissue distribution and cellular identification in skin

Kupczyk

Rykała

Serek

et al. 2022

Veterinary Internal Medicne

View full text Add to dashboard Cite

Background: The endocannabinoid system (ECS) is composed of cannabinoid receptors type 1 (CBR1) and type 2 (CBR2), cannabinoid-based ligands (endogenous chemically synthesized phytocannabinoids), and endogenous enzymes controlling their concentrations. Cannabinoid receptors (CBRs) have been identified in invertebrates and in almost all vertebrate species in the central and peripheral nervous system as well as in immune cells, where they control neuroimmune homeostasis. In humans, rodents, dogs, and cats, CBRs expression has been confirmed in the skin, and their expression and tissue distribution become disordered in pathological conditions. Cannabinoid receptors may be a possible therapeutic target in skin diseases.Objectives: To characterize the distribution and cellular expression of CBRs in the skin of horses under normal conditions. Animals: Fifteen healthy horses.Methods: Using full-thickness skin punch biopsy samples, skin-derived primary epidermal keratinocytes and dermal-derived cells, we performed analysis of Cnr1 and

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

“…The equine dermis has a heterogeneous cell population of which fibroblasts are the primary cells supported by skin dendrocytes, principal elements of the equine cutaneous immune system. 31 The dermis is subdivided into the superficial papillary and deep reticular dermis. In However, although rabbit polyclonal antibodies for CBRs, applied during confocal microscopy, allowed us to demonstrate CBRs distribution in the tissues, verification to assess their specificity, as well as quantification of the CBRs, was performed using a WB method in the whole skin tissue and brain samples (Figure 4).…”

Section: Dermal Expression Of Cbrsmentioning

confidence: 99%

See 1 more Smart Citation

The cannabinoid receptors system in horses: Tissue distribution and cellular identification in skin

Kupczyk

Rykała

Serek

et al. 2022

Veterinary Internal Medicne

View full text Add to dashboard Cite

show abstract

“…In addition, Mittmann et al had previously identified several candidate genes under the hypothesis of an overregulated inflammatory autoimmune response, two of which were confirmed to lie within regions associated with CPL (ubiquitin protein ligase E3A and CD109 on ECA1 and ECA10, respectively) [ 10 , 11 ]. Further functional research is needed to identify the genetic cause underlying CPL and the involvement of the immune system [ 34 ].…”

Section: Pathogenesis: Two Diverging Hypothesesmentioning

confidence: 99%

“…None of the conducted studies have identified a specific genetic marker and/or mutation that is correlated with CPL. Different candidate genes have been suggested, but further functional research is needed to identify the genetic cause underlying CPL [ 34 ].…”

Section: Diagnosismentioning

confidence: 99%

Chronic Progressive Lymphedema in Belgian Draft Horses: Understanding and Managing a Challenging Disease

Bryś

Claerebout

Chiers

2023

Veterinary Sciences

View full text Add to dashboard Cite

Chronic progressive lymphedema (CPL) in draft horses is characterized by increased dermal thickness and fibrosis, with the development of skinfolds and nodules, hyperkeratosis, and ulcerations on the distal limbs of affected horses. Secondary bacterial, fungal, or parasitic infections frequently complicate and aggravate the lesions, as well as the progression of this disease. CPL has a particularly high prevalence of up to 85.86% in the Belgian draft horse breed. Due to the disease’s progressive and incurable nature, affected horses are often euthanized prematurely. The treatment options are solely symptomatic, aimed at improving the horse’s quality of life. Despite the severity of this condition, many uncertainties about its etiology and pathogenesis still remain to date. The established scientific research on CPL is rather limited, although there is an urgent need for strategies to tackle this disease. This review summarizes the available knowledge, serving as a guideline for practitioners, and provides perspectives for future research programs.

show abstract

Allergen immunotherapy using recombinant Culicoides allergens improves clinical signs of equine insect bite hypersensitivity

Graner,

Mueller,

Geisler

et al. 2024

Front. Allergy

View full text Add to dashboard Cite

IntroductionInsect bite hypersensitivity (IBH) is an IgE-mediated allergic dermatitis of horses caused by bites of Culicoides spp., sharing some common features with human atopic dermatitis. Allergen immunotherapy (AIT) using Culicoides whole-body extracts has limited efficacy. This study aimed to evaluate AIT with a pool of major Culicoides recombinant allergens in a prospective, double-blinded, placebo-controlled study.MethodsThe IBH lesion score was assessed during a pre-treatment year and first treatment year (May–October) in 17 horses and in May and July of a second treatment year. Nine horses were immunized subcutaneously 3× with a combination of nine r-allergens (20 μg each/injection) in alum and monophosphoryl lipid A (MPLA). Eight horses received a placebo. The immunization was repeated twice the following year. The specific antibody response to one of the AIT Culicoides r-allergens was assessed.ResultsIn the first treatment year, the decrease in average IBH lesion score was significantly larger in the AIT compared to the placebo group, with 67% of the AIT group and 25% of the placebo horses reaching >50% improvement of the average IBH lesion score. The response to the AIT was enhanced in the 2nd treatment year when 89% of the AIT vs. 14% of the placebo horses showed an improvement (p ≤ 0.01). IgG antibodies of all subclasses were induced, with IgG4/7 showing the most significant differences between groups. The post-AIT sera showed IgE blocking activity.DiscussionAIT using only a few injections of small amounts of r-allergens in alum and MPLA as immunomodulators seems a promising approach for the treatment of insect bite allergy.

show abstract

Genetics of Skin Disease in Horses

Cited by 6 publications

References 74 publications

The cannabinoid receptors system in horses: Tissue distribution and cellular identification in skin

The cannabinoid receptors system in horses: Tissue distribution and cellular identification in skin

Chronic Progressive Lymphedema in Belgian Draft Horses: Understanding and Managing a Challenging Disease

Allergen immunotherapy using recombinant Culicoides allergens improves clinical signs of equine insect bite hypersensitivity

Contact Info

Product

Resources

About