Genetics of host-pathogen interactions in the Pyrenophora teres f. teres (net form) – barley (Hordeum vulgare) pathosystem

Afanasenko, Olga; Мироненко, Н. В.; Filatova, O.; Kopahnke, Doris; Krämer, Ilona; Ordon, Frank

doi:10.1007/s10658-006-9093-5

Cited by 26 publications

(22 citation statements)

References 19 publications

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“…The virulence of mating progeny populations was studied on selected barley genotypes from a barley differential set previously used (Robinson & Jalli, 1996;, 2000. Barley genotypes CI 5791, CI 5822 and Manchuria carry 1-3 net blotch resistance genes (Steffenson & Webster, 1992;Afanasenko et al, 2007), Pohto has quantitative resistance and Arve is a susceptible variety (Robinson & Jalli, 1999). The resistant reaction of barley genotypes to the parental net form isolates was similar to previous studies (Robinson & Jalli, 1996).…”

Section: Discussionsupporting

confidence: 79%

“…Necrotrophic pathogens have been reported to produce host-specific toxins (HST) that may function as effectors in inducing toxicity and disease in the host species following a gene-for-gene model (Friesen et al, 2008). There is strong evidence for a gene-for-gene interaction in the P. teres-barley pathosystem (Afanasenko et al, 2007). Lai et al (2007) identified three major avirulence genes in P. teres f. teres involved in avirulence/virulence of the fungus: AvrHar, AvrPra1 and AvrPra2, and Beattie et al (2007) identified six amplified fragment length polymorphism (AFLP) markers linked to a P. teres f. teres avirulence gene AvrHeartland.…”

Section: Introductionmentioning

confidence: 99%

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Sexual reproduction and soil tillage effects on virulence of Pyrenophora teres in Finland

Jalli

2010

Annals of Applied Biology

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Knowledge of the virulence of a pathogen population and recognition of the risks of changes in the virulence spectrum are essential in breeding crops for disease resistance. Sexual recombination in a pathogen increases the level of genotypic diversity and can influence the virulence spectrum. This study aimed to determine how sexual recombination can change virulence of the barley pathogen Pyrenophora teres and whether the barley cultivation system, no-tillage or normal tillage, influences P. teres virulence. The inheritance of avirulence/virulence in P. teres following sexual reproduction was studied in three artificially created pathogen populations. The first was a product of crossing two net forms of the pathogen, and the second and the third were products of crossing net and spot forms. None of the progeny generated resembled the parents exactly. The average similarity of the progeny isolates of the net by net cross with the parental type, based on avirulence/virulence tests, was 92%. That for net and spot form progenies was 58% in comparison with the net form parents and 73% with the spot form parents. The virulence reactions of the progeny isolates did not correlate with morphological traits of the isolates: growth rate on agar, spore production, spore width, spore length and numbers of septa per conidium. To study the effect of the barley cultivation method on P. teres virulence, 313 single-spore cultures were obtained from barley fields. Two hundred and seventy-six of the isolates represented the spot form and 37 represented the net form of P. teres. No association was established between the tillage method and virulence for either the net form or the spot form isolates.

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Section: Discussionsupporting

confidence: 79%

Section: Introductionmentioning

confidence: 99%

Sexual reproduction and soil tillage effects on virulence of Pyrenophora teres in Finland

Jalli

2010

Annals of Applied Biology

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“…This result agrees well with previous reports on NFNB where one to three major genes, in addition to minor genes, have been shown to control NFNB resistance/susceptibility in barley in any given biparental population (reviewed in Liu et al ., ). Current and previous genetic studies using fungal mapping populations have also shown that a few major genes in each P. teres f. teres isolate condition virulence/avirulence towards a particular host genotype (Afanasenko et al ., ; Beattie et al ., ; Lai et al ., ; Shjerve et al ., ).…”

Section: Discussionmentioning

confidence: 99%

Necrotrophic effector‐triggered susceptibility (NETS) underlies the barley–Pyrenophora teres f. teres interaction specific to chromosome 6H

Liu

Holmes

Faris

et al. 2014

Molecular Plant Pathology

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Barley net form net blotch (NFNB), caused by the necrotrophic fungus Pyrenophora teres f. teres, is a destructive foliar disease in barley-growing regions worldwide. Little is known about the genetic and molecular basis of this pathosystem. Here, we identified a small secreted proteinaceous necrotrophic effector (NE), designated PttNE1, from intercellular wash fluids of the susceptible barley line Hector after inoculation with P. teres f. teres isolate 0-1. Using a barley recombinant inbred line (RIL) population developed from a cross between the sensitive/susceptible line Hector and the insensitive/resistant line NDB 112 (HN population), sensitivity to PttNE1, which we have named SPN1, mapped to a common resistance/susceptibility region on barley chromosome 6H. PttNE1-SPN1 interaction accounted for 31% of the disease variation when the HN population was inoculated with the 0-1 isolate. Strong accumulation of hydrogen peroxide and increased levels of electrolyte leakage were associated with the susceptible reaction, but not the resistant reaction. In addition, the HN RIL population was evaluated for its reactions to 10 geographically diverse P. teres f. teres isolates. Quantitative trait locus (QTL) mapping led to the identification of at least 10 genomic regions associated with disease, with chromosomes 3H and 6H harbouring major QTLs for resistance/susceptibility. SPN1 was associated with all the 6H QTLs, except one. Collectively, this information indicates that the barley-P. teres f. teres pathosystem follows, at least partially, an NE-triggered susceptibility (NETS) model that has been described in other necrotrophic fungal disease systems, especially in the Dothideomycete class of fungi.

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“…Among them, Canadian Lake Shore CI2750, Tifang CI4407-1, Manchurian M-CI2330, Manchurian CI5791, Manchurian CI1251, Harbin CI4929, CIho4922, CIho5822, Atlas CI4118, Rojo CI5401, Coast CI2235, and Ming CI4797 haven been reported and used as the International Differential set previously (Afanasenko et al 2007;Afanasenko et al 2009). Seed of the international differential set was obtained from Genetic Resource Unit of ICARDA.…”

Section: Plant Materialsmentioning

confidence: 99%

Virulence of MoroccanPyrenophora teresf.teresRevealed by International Differential Barley Genotypes

Taibi

Bentata

Rehman³

et al. 2016

Cereal Research Communications

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Pyrenophora teres f. teres (Ptt), causing net blotch in barley, is an important and frequently isolated leaf pathogen across the globe. The virulence spectrum of Ptt from North Africa including Morocco is poorly understood. Sixteen barley genotypes were challenged, at seedling stage, with 15 Ptt isolates that were collected from different agroecological zones of Morocco. The experiment was conducted in a factorial arrangement of treatments in a randomized complete block design with three replicates. The ANOVA revealed highly significant (P < 0.001) effects of genotype (G), isolate (I) and G×I interaction explaining 23.2, 62.5, and 13.9% of the variation, respectively. Therefore, the current study revealed highly diverse virulence pattern of Moroccan isolates. Furthermore, the results indicated that minor virulence of Ptt isolates dominated over virulence interaction. In addition, Taffa (6-rowed) and Aglou (2 rowed), had the highest level of resistance to Ptt, while Coast and Rabat071 were the most susceptible genotypes. Pt2, Pt7, Pt8 and Pt4 were being the most virulent isolates, while Pt10 and Pt11 were the least virulent isolates. The emergence of the new Ptt pathotypes, which were highly virulent to durable resistance in Rabat071 posed a risk of breaking down the currently deployed resistance to net blotch in Morocco. A careful evaluation and selection of Ptt isolates based on minor virulence pattern to barley genotypes is essential for successful barley breeding program for resistance to net blotch in Morocco.

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Genetics of host-pathogen interactions in the Pyrenophora teres f. teres (net form) – barley (Hordeum vulgare) pathosystem

Abstract: The genetics of host-pathogen interactions in the Hordeum vulgare -P. teres f. teres pathosystem was studied in twelve resistant barley accessions, i.e. CI

Cited by 26 publications

References 19 publications

Sexual reproduction and soil tillage effects on virulence of Pyrenophora teres in Finland

Sexual reproduction and soil tillage effects on virulence of Pyrenophora teres in Finland

Necrotrophic effector‐triggered susceptibility (NETS) underlies the barley–Pyrenophora teres f. teres interaction specific to chromosome 6H

Virulence of MoroccanPyrenophora teresf.teresRevealed by International Differential Barley Genotypes

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