Genetics of hemolysin of Escherichia coli

Hull, Sheila I.; Hull, Richard A.; Minshew, Barbara H.; Falkow, Stanley

doi:10.1128/jb.151.2.1006-1012.1982

Cited by 47 publications

(22 citation statements)

References 40 publications

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“…E. coli J96 (serotype 04), like strain C1212, is an upper urinary tract isolate expressing both Hly and MRHA activities (12). A significant number of J96 cosmid derivatives (5 of 50) which expressed Hly also expressed MRHA and Syn [Gal-Gal]-latex agglutination.…”

Section: Resultsmentioning

confidence: 99%

“…Hly was shown initially to be plasmid encoded in E. coli isolated from animals (4). A related gene has been recently shown to be chromosomally encoded in most human urinary tract isolates (12,19). Because a larger proportion of human extraintestinal E. coli isolates produce Hly (35 to 60%) relative to normal fecal isolates (10%), it has been proposed that this determinant may play a role in the pathogenesis of extraintestinal E. coli human infections (10,18).…”

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confidence: 99%

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Gene clusters governing the production of hemolysin and mannose-resistant hemagglutination are closely linked in Escherichia coli serotype O4 and O6 isolates from urinary tract infections

Low¹,

David²,

Lark³

et al. 1984

Infect Immun

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113

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The genes encoding alpha-hemolysin and mannose-resistant hemagglutination were shown to be closely linked in cloned DNA from two Escherichia coli urinary tract isolates of serotypes 04 (J96) and 06 (C1212). DNA hybridization experiments demonstrated that the hly and mrh gene clusters of other E. coli 06 serotypes were also linked. Colony hybridizations showed that most normal fecal E. coli do not contain hly and mrh DNA but much of the intervening DNA between these two gene clusters is common among all E. coli. We have further demonstrated that there is a small (about 1 kilobase) region of homology located on both sides of the hly sequence and present elsewhere in the C1212 strain. We suggest that linkage of hly and mrh occurred through a transposition event, and we discuss the potential significance of this linkage in the acquisition of virulence determinants by these bacteria.

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Section: Resultsmentioning

confidence: 99%

mentioning

confidence: 99%

Gene clusters governing the production of hemolysin and mannose-resistant hemagglutination are closely linked in Escherichia coli serotype O4 and O6 isolates from urinary tract infections

Low¹,

David²,

Lark³

et al. 1984

Infect Immun

Self Cite

113

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show abstract

“…Five such islands have previously been identified in pathogenic E. coli isolates (10,14). PAIs I, II, IV, and V carry a number of virulence gene clusters, among them the P (pap) and P-related (prf) fimbria gene clusters and two alpha-hemolysin loci, hlyI and hlyII (2,3,16,21). The locus LEE (locus of enterocyte effacement) was identified in an EPEC strain (23); LEE carries a different set of virulence genes than those found in PAI I, II, IV, or V but is inserted in the same chromosomal site as PAI I, at 82 min at the selenocysteine-specific tRNA (2,23).…”

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Chromosomal Regions Specific to Pathogenic Isolates of Escherichia coli Have a Phylogenetically Clustered Distribution

1998

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We studied the ancestry of virulence-associated genes inEscherichia coli by examining chromosomal regions specific to pathogenic isolates. The four virulence determinants examined were the alpha-hemolysin (hly) loci hlyI andhlyII, the type II capsule gene cluster kps, and the P (pap) and S (sfa) fimbria gene clusters. All four loci were shown previously to be associated with pathogenicity islands of uropathogenic E. coli isolates. The hly, kps, sfa, andpap regions each have an unexpected clustered distribution among the E. coli collection of reference (ECOR) strains, but all these regions were absent from a collection of diarrheagenic E. coli isolates. Strains in the ECOR subgroup B2 typically had a combination of at least three of the four loci, and all strains in subgroup D had a copy of the kpsand pap clusters. In contrast, only four strains in subgroup A had either hly, kps,sfa, or pap, and no subgroup A strains had all four together. Strains of subgroup B1 were devoid of all four virulence regions, with the exception of one isolate that had a copy of thesfa gene cluster. This phylogenetic distribution of strain-specific sequences corresponds to the ECOR groups with the largest genome size, namely, B2 and D. We propose that the pathogenicity islands are ancestral to subgroups B2 and D and were acquired after speciation, with subsequent horizontal transfer into some group A, B1, and E lineages. These results suggest that thehly, kps, sfa, and pappathogenicity determinants may play a role in the evolution of enteric bacteria quite apart from, and perhaps with precedence over, their ability to cause disease.

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“…The genes for the hemolytic property are either located on transmissible plasmids (3-5, 7, 19) or on the bacterial chromosome (1,5,11,12,14,15,20). Hly plasmids belong to different incompatibility groups (3) and have been isolated from various sources.…”

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Relation between the hemolytic property and iron metabolism in Escherichia coli

Lebek¹,

Gruenig²

1985

Infect Immun

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The hemolytic activity of wild-type strqips of Escherichia coli was measured by a standardized method in liquid broth. The system also allowed us to investigate the influence of various Fe3+ concentrations in the cultures on the amount of secreted hemolysin. We found that the ,hemolysin secretion of all strains was clearly reduced after addition of FeCl3. However, the influ,ence of additional iron chelators showed remarkable differepces. The hemolytic activity of Hly plasmid-containing strains isolated in Berne significantly increased. Most of the strains w;th a chromosomal hemolysin determinant showed a similar effect, but to a lesser degree.

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Genetics of hemolysin of Escherichia coli

Cited by 47 publications

References 40 publications

Gene clusters governing the production of hemolysin and mannose-resistant hemagglutination are closely linked in Escherichia coli serotype O4 and O6 isolates from urinary tract infections

Gene clusters governing the production of hemolysin and mannose-resistant hemagglutination are closely linked in Escherichia coli serotype O4 and O6 isolates from urinary tract infections

Chromosomal Regions Specific to Pathogenic Isolates of Escherichia coli Have a Phylogenetically Clustered Distribution

Relation between the hemolytic property and iron metabolism in Escherichia coli

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