Genetics of female infertility: Molecular study of newborn ovary homeobox gene in poor ovarian responders

Batiha, Osamah; Alahmad, Nour Alhoda; Sindiani, Amer; Bodoor, Khaldon; Shaaban, Sherin; Al-Smadi, Mohammad

doi:10.4103/jhrs.jhrs_112_18

Cited by 8 publications

(3 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The cohort analyzed in this study has been described in detail elsewhere [ 14 ]. To summarize, 60 female partners of selected couples undergoing ART for infertility were enrolled in the present study.…”

Section: Methodsmentioning

confidence: 99%

Association of single-nucleotide polymorphisms in the ESR2 and FSHR genes with poor ovarian response in infertile Jordanian women

Sindiani

Batiha

Al‐zoubi

et al. 2021

Clin Exp Reprod Med

Self Cite

View full text Add to dashboard Cite

Objective: Poor ovarian response (POR) refers to a subnormal follicular response that leads to a decrease in the quality and quantity of the eggs retrieved after ovarian stimulation during assisted reproductive treatment (ART). The present study investigated the associations of multiple variants of the estrogen receptor 2 (ESR2) and follicle-stimulating hormone receptor (FSHR) genes with POR in infertile Jordanian women undergoing ART.Methods: Four polymorphisms, namely ESR2 rs1256049, ESR2 rs4986938, FSHR rs6165, and FSHR rs6166, were investigated in 60 infertile Jordanian women undergoing ART (the case group) and 60 age-matched fertile women (the control group), with a mean age of 33.60±6.34 years. Single-nucleotide polymorphisms (SNPs) were detected by restriction fragment length polymorphism and then validated using Sanger sequencing.Results: The p-value of the difference between the case and control groups regarding FSHR rs6166 was very close to 0.05 (p=0.054). However, no significant differences were observed between the two groups in terms of the other three SNPs, namely ESR2 rs1256049, ESR2 rs4986938, and FSHR rs6165 (p=0.561, p=0.433, and p=0.696, respectively).Conclusion: The association between FSHR rs6166 and POR was not statistically meaningful in the present study, but the near-significant result of this experiment suggests that statistical significance might be found in a future study with a larger number of patients.

show abstract

Section: Methodsmentioning

confidence: 99%

Association of single-nucleotide polymorphisms in the ESR2 and FSHR genes with poor ovarian response in infertile Jordanian women

Sindiani

Batiha

Al‐zoubi

et al. 2021

Clin Exp Reprod Med

Self Cite

View full text Add to dashboard Cite

show abstract

“…The aforesaid anti-hormonal effects could inhibit endometrial DNA synthesis and cause a decrease in endometrial receptivity, while the depletion of estrogen levels could lead to increased cervical viscosity, which is not conducive to sperm passage. Therefore, any long-term use of ovulation promoting drugs tends to lower pregnancy rates and increase early abortion rates [21][22].…”

Section: The Mechanisms Of De-t1 On Reproduction Healthmentioning

confidence: 99%

DE-T1 on the Blastocyst obtained Rate and Live Births Rates in Women Receiving IVF-ET Treatment

Shao¹,

Yamaguchi²,

Nozaki³

et al. 2022

J Women's Health Dev

View full text Add to dashboard Cite

BackgroundOur objective was to retrospectively analyze the in uence of DE-T1, a type of amino-polysaccharide extracted from dandelions, on the rates of blastocysts obtained and live births in women undergoing IVF-ET treatment. MethodsThis was a retrospective cohort study, conducting a total of 1014 patients over the age of 30, who received IVF treatment at Hanabusa Women's clinic from Aug. 1, 2012 to Feb. 29, 2020. The patients were divided into two groups, based on their own choice regarding DE-T1 supplementation, which is available as over the counter medicine at the Clinic. The two groups' overall rates of blastocysts obtained were compared and the rates of blastocysts obtained in patients with different ages and Anti-Mullerian hormone (AMH) levels were also compared using an Intention-to-treat (ITT) analysis. Among the patients who completed embryo transfers (ET), the live birth rate was compared between the supplementation group and the non-supplementation group using a Chi-squared test. ResultsThe blastocyst obtained rate in the supplementation group was 75.98%, which was signi cantly higher than that of 57.28% in the non-supplementation group (P=2.4×10 9 ). The blastocyst obtained rate across the range of ages in the supplementation group were signi cantly higher than those of the nonsupplementation group (≥30 and <35 years of age: 90.97% vs 74.32%, P=0.001;≥35 and <40 years of age: 82.40% vs 69.79%, P= 0.010; ≥40 and <43 years of age: 72.90% vs 52.11%, P=0.002; ≥43 years of age: 53.29% vs 22.95%, P= 5.7×10 5 ). The blastocyst obtained rate for both AMH levels in the supplementation group were signi cantly higher than those of the non-supplementation group (AMH≤1.1: 56.47% vs 40.44%, P=0.002; AMH> 1.1: 88.48% vs 71.08%, P=2.52×10 7 ). The live birth rate of the supplementation group was signi cantly higher than that of the non-supplementation group (57.53% vs. 40.0%, P=0.045). ConclusionDE-T1 supplementation might be an in uence on improving the blastocyst obtained rate and live birth rates in women receiving IVF-ET treatment. DE-T1 supplementation might be of bene t to women of different ages and AMH levels.

show abstract

“…The newborn ovary homeobox (NOBOX) gene, an oocyte-specific homeobox gene, transcriptionally regulates oocyte-specific genes that play key roles in ovarian development, early oogenesis, and fertility (5)(6)(7). Research on the NOBOX gene has focused on its role in ovarian development and oogenesis.…”

Section: Introductionmentioning

confidence: 99%

Association between polymorphisms in NOBOX and litter size traits in Xiangsu pigs

Huang,

Ruan,

Xiao

et al. 2024

Front. Vet. Sci.

View full text Add to dashboard Cite

The newborn ovary homeobox gene (NOBOX) regulates ovarian and early oocyte development, and thus plays an essential role in reproduction. In this study, the mRNA expression level and single nucleotide polymorphism (SNP) of NOBOX in various tissues of Xiangsu pigs were studied to explore the relationship between its polymorphism and litter size traits. Also, bioinformatics was used to evaluate the effects of missense substitutions on protein structure and function. The results revealed that NOBOX is preferentially expressed in the ovary. Six mutations were detected in the NOBOX sequence, including g.1624 T>C, g.1858 G>A, g.2770 G>A, g.2821 A>G, g.5659 A>G, and g.6025 T>A, of which g.1858 G>A was a missense mutation. However, only g.1858 G>A, g.5659 A>G, and g.6025 T>A were significantly associated with litter size traits (p < 0.05). Further prediction of the effect of the missense mutation g.1858 G>A on protein function revealed that p.V82M is a non-conservative mutation that significantly reduces protein stability and thus alters protein function. Overall, these findings suggest that NOBOX polymorphism is closely related to the litter size of Xiangsu pigs, which may provide new insights into pig breeding.

show abstract

Genetics of female infertility: Molecular study of newborn ovary homeobox gene in poor ovarian responders

Cited by 8 publications

References 32 publications

Association of single-nucleotide polymorphisms in the ESR2 and FSHR genes with poor ovarian response in infertile Jordanian women

Association of single-nucleotide polymorphisms in the ESR2 and FSHR genes with poor ovarian response in infertile Jordanian women

DE-T1 on the Blastocyst obtained Rate and Live Births Rates in Women Receiving IVF-ET Treatment

Association between polymorphisms in NOBOX and litter size traits in Xiangsu pigs

Contact Info

Product

Resources

About