Genetics and Ecology of Isoprene Degradation

Crombie, Andrew T.; Mejia-Florez, Nasmille L.; McGenity, Terry J.; Murrell, J. Colin

doi:10.1007/978-3-319-39782-5_27-1

Cited by 4 publications

(2 citation statements)

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“…Various bacterial isolates, predominantly of the phyla Actinobacteriota and Proteobacteriota , can utilize isoprene as a carbon and energy source under oxic conditions ( 1 , 8 ). Genome analysis showed that actinobacterial isolates belonging to the genera Rhodococcus , Mycobacterium , and Gordonia all possess two linked operons (i.e., isoABCDEF , isoGHIJ ) responsible for the stepwise conversion of isoprene via 1,2-epoxy-isoprene and a glutathione conjugate 1-hydroxy-2-glutathionyl-2-methyl-3-butene ( 8 , 11 , 13 – 16 ). Many aerobic isoprene-degrading microorganisms have been isolated from soil and the leaves of isoprene-producing trees (e.g., poplar, willow), including Gordonia , Nocardia , Methylobacterium , Pseudomonas , Klebsiella , Alcaligenes , Arthrobacter , Variovorax , Ramlibacter , Sphingopyxis , Sphingobacterium , Sphingobium , Leifsonia , Micrococcus , Mycobacterium , Nocardioides , Loktanella , Shinella , Stappia , Pantoea , Bacillus , and Rhodococcus strains ( 8 ), indicating that a diversity of aerobic bacteria contributes to isoprene turnover.…”

Section: Introductionmentioning

confidence: 99%

“…To date, few studies have reported on isoprene-degrading anaerobes, and isolates capable of isoprene degradation and transformation under anoxic conditions are not available. The contributions of anaerobic isoprene degradation and transformation are uncertain and possibly overlooked processes in isoprene cycling in the environment ( 8 , 13 ). A prior study observed that methanogenic enrichment cultures containing Methanospirillum hungatei and Methanothrix soehngenii converted unsaturated hydrocarbons, including squalene and 1-hexadecene, to methane and carbon dioxide, but isoprene was not transformed ( 17 ).…”

Section: Introductionmentioning

confidence: 99%

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Anaerobic Biohydrogenation of Isoprene by Acetobacterium wieringae Strain Y

Jin

Wang

et al. 2022

mBio

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Anaerobic Biohydrogenation of Isoprene by Acetobacterium wieringae Strain Y

Jin

Wang

et al. 2022

mBio

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Possible links between soil variables, bacterial abundance and kinetic constants in isoprene degradation by dry deciduous tropical forest soils

Singh

Dubey

2023

Eur J Forest Res

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Gene probing reveals the widespread distribution, diversity and abundance of isoprene-degrading bacteria in the environment

et al. 2018

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BackgroundApproximately 500 Tg of isoprene are emitted to the atmosphere annually, an amount similar to that of methane, and despite its significant effects on the climate, very little is known about the biological degradation of isoprene in the environment. Isolation and characterisation of isoprene degraders at the molecular level has allowed the development of probes targeting isoA encoding the α-subunit of the isoprene monooxygenase. This enzyme belongs to the soluble diiron centre monooxygenase family and catalyses the first step in the isoprene degradation pathway. The use of probes targeting key metabolic genes is a successful approach in molecular ecology to study specific groups of bacteria in complex environments. Here, we developed and tested a novel isoA PCR primer set to study the distribution, abundance, and diversity of isoprene degraders in a wide range of environments.ResultsThe new isoA probes specifically amplified isoA genes from taxonomically diverse isoprene-degrading bacteria including members of the genera Rhodococcus, Variovorax, and Sphingopyxis. There was no cross-reactivity with genes encoding related oxygenases from non-isoprene degraders. Sequencing of isoA amplicons from DNA extracted from environmental samples enriched with isoprene revealed that most environments tested harboured a considerable variety of isoA sequences, with poplar leaf enrichments containing more phylogenetically diverse isoA genes. Quantification by qPCR using these isoA probes revealed that isoprene degraders are widespread in the phyllosphere, terrestrial, freshwater and marine environments. Specifically, soils in the vicinity of high isoprene-emitting trees contained the highest number of isoprene-degrading bacteria.ConclusionThis study provides the molecular ecology tools to broaden our knowledge of the distribution, abundance and diversity of isoprene degraders in the environment, which is a fundamental step necessary to assess the impact that microbes have in mitigating the effects of this important climate-active gas.Electronic supplementary materialThe online version of this article (10.1186/s40168-018-0607-0) contains supplementary material, which is available to authorized users.

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Genetics and Ecology of Isoprene Degradation

Cited by 4 publications

References 57 publications

Anaerobic Biohydrogenation of Isoprene by Acetobacterium wieringae Strain Y

Anaerobic Biohydrogenation of Isoprene by Acetobacterium wieringae Strain Y

Possible links between soil variables, bacterial abundance and kinetic constants in isoprene degradation by dry deciduous tropical forest soils

Gene probing reveals the widespread distribution, diversity and abundance of isoprene-degrading bacteria in the environment

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