Genetics and biology of coloration in reptiles: the curious case of the Lemon Frost geckos
Longhua Guo,
Leonid Kruglyak
Abstract:Although there are more than 10,000 reptile species, and reptiles have historically contributed to our understanding of biology, genetics research into class Reptilia has lagged compared with other animals. Here, we summarize recent progress in genetics of coloration in reptiles, with a focus on the leopard gecko, Eublepharis macularius. We highlight genetic approaches that have been used to examine variation in color and pattern formation in this species as well as to provide insights into mechanisms underlyi… Show more
“…Some factors, such as temperature and stress, that may affect color patterns and are mediated by iridophores have been investigated (25,29). In general, advances in genome sequencing allow for tackling genetic mechanisms of coloration in reptiles from a genomic perspective, brie y reviewed by Guo and Kruglyak (30). However, the mechanisms that may cause the lack of iridophores are still insu ciently known in squamates and are obscure in Galápagos iguanas.…”
Critically Endangered Galápagos pink land iguanas (Conolophus marthae) are one of the rarest species of iguana, found exclusively on Isabela Island, in the Galápagos. A previous histological study provided an explanation of the unique pink color of adult C. marthae. Still, it also raised a question related to the recent discovery that C. marthae hatchlings are not pink upon emergence. Like hatchlings of C. subcristatus, another Galápagos iguana species, they are maculated, but contrary to C. subcristatus, C. marthae hatchlings exhibit a green color pattern. Generally, greenish, and bluish colors in reptiles are primarily due to an interaction between different types of chromatophores in different skin layers. Iridophores are particularly important among chromophores, as they contain guanine nanocrystals capable of reflecting and diffracting light. Green color emerges when the mostly diffracted blue wavelength interferes with carotenoids in xanthophores. In a multiapproach analysis, we investigated the histological, ultrastructural and immunohistochemical features of differently-colored skin areas in three species of Galápagos iguanas. We tested for the presence of different types of chromatophores and described characteristics of stratus corneum, stratus germinativum and stratum laxum in the different species. Results demonstrated the absence of iridophores in the skin of adults of the three species, regardless of color, and the presence of stratified epithelium in the stratum germinativum of C. marthae non-black-colored skin. This research furthers interest in the evolutionary significance of pink coloration in C. marthae. The study also calls for additional research to demonstrate the presence of iridophores in pink iguana hatchlings and document possible ontogenetic changes that may lead to the lack of iridophores in adults. An investigation that could take advantage of genome mapping and gene expression along a developmental gradient would be greatly beneficial for advancing the understanding of the genomic basis of pink iguanas and, in general, reptile coloration.
“…Some factors, such as temperature and stress, that may affect color patterns and are mediated by iridophores have been investigated (25,29). In general, advances in genome sequencing allow for tackling genetic mechanisms of coloration in reptiles from a genomic perspective, brie y reviewed by Guo and Kruglyak (30). However, the mechanisms that may cause the lack of iridophores are still insu ciently known in squamates and are obscure in Galápagos iguanas.…”
Critically Endangered Galápagos pink land iguanas (Conolophus marthae) are one of the rarest species of iguana, found exclusively on Isabela Island, in the Galápagos. A previous histological study provided an explanation of the unique pink color of adult C. marthae. Still, it also raised a question related to the recent discovery that C. marthae hatchlings are not pink upon emergence. Like hatchlings of C. subcristatus, another Galápagos iguana species, they are maculated, but contrary to C. subcristatus, C. marthae hatchlings exhibit a green color pattern. Generally, greenish, and bluish colors in reptiles are primarily due to an interaction between different types of chromatophores in different skin layers. Iridophores are particularly important among chromophores, as they contain guanine nanocrystals capable of reflecting and diffracting light. Green color emerges when the mostly diffracted blue wavelength interferes with carotenoids in xanthophores. In a multiapproach analysis, we investigated the histological, ultrastructural and immunohistochemical features of differently-colored skin areas in three species of Galápagos iguanas. We tested for the presence of different types of chromatophores and described characteristics of stratus corneum, stratus germinativum and stratum laxum in the different species. Results demonstrated the absence of iridophores in the skin of adults of the three species, regardless of color, and the presence of stratified epithelium in the stratum germinativum of C. marthae non-black-colored skin. This research furthers interest in the evolutionary significance of pink coloration in C. marthae. The study also calls for additional research to demonstrate the presence of iridophores in pink iguana hatchlings and document possible ontogenetic changes that may lead to the lack of iridophores in adults. An investigation that could take advantage of genome mapping and gene expression along a developmental gradient would be greatly beneficial for advancing the understanding of the genomic basis of pink iguanas and, in general, reptile coloration.
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