Genetically Related Protein Variants Specifically Associated with Fruiting Body Maturation in
            <i>Neurospora</i>

Nasrallah, June B.; Srb, Adrian M.

doi:10.1073/pnas.70.6.1891

Cited by 25 publications

(25 citation statements)

References 8 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Biochemical analysis of components of the Neurospora life cycle has revealed, in N. crassa, N. sitophila, N. tetrasperma, and N. terricola, a specific association between fruiting body (perithecial) development and a major protein species resolvable on polyacrylamide gels (1,2). This-protein can be detected in unfertilized fruiting bodies, but its concentration increases drastically in fertilized fruiting bodies until 4 or 5 days after fertilization, when it constitutes a major fraction of the total perithecial proteins.…”

mentioning

confidence: 99%

“…This-protein can be detected in unfertilized fruiting bodies, but its concentration increases drastically in fertilized fruiting bodies until 4 or 5 days after fertilization, when it constitutes a major fraction of the total perithecial proteins. Later in maturation, as perithecial contents are lost in the perithecial exudate and subsequently in spore discharge, the levels of the major perithecial protein decrease (1,2). Detailed reports of this type of perithecial protein have so far been limited to the usual laboratory species, N. crassa and N. tetrasperma.…”

mentioning

confidence: 99%

“…Harvesting and extraction of samples on 0.1M phosphate buffer (pH 7.0) and electrophoresis on 7.5% polyacrylamide gels were as described earlier (1).…”

mentioning

confidence: 99%

See 2 more Smart Citations

Occurrence of a major protein associated with fruiting body development in Neurospora and related Ascomycetes.

Nasrallah¹,

Srb²

1977

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

show abstract

mentioning

confidence: 99%

mentioning

confidence: 99%

See 1 more Smart Citation

Occurrence of a major protein associated with fruiting body development in Neurospora and related Ascomycetes.

Nasrallah¹,

Srb²

1977

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

show abstract

“…Perithecia maturation includes differentiation of the perithecial wall and internal development of asci containing ascospores. By use of appropriate mutants, loci that regulate development of one phase only (1,2) as well as loci regulating both phases (3) have been identified. A number of loci of the second type regulate morphology of both asci and vegetative hyphae.…”

mentioning

confidence: 99%

“…When testing the linearity of the 3-methyl-2-benzothiazolinone hydrazone (MBTH) assay to HAG concentration, the chloroform-washed sample was precipitated with 1 Fractionation. An aliquot of the HAG sample was applied to a column of Sephadex G-50 (fine) (100-cm bed height in a 1.6-cm diameter column) equilibrated with 5 mM TES, pH 8.0, in order to desalt the sample and equilibrate it to the TES buffer for ion exchange fractionation.…”

mentioning

confidence: 99%

Molecular alteration in a Neurospora crassa morphological mutant and its phenocopy.

Springer¹,

Srb²

1978

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

A procedure using ion exchange chromatography has been developed to detect alterations in a polysaccharide produced by Neurospora crassa. The polysaccharide, isolated from medium that has supported the growth of a culture, is highly responsive to the 3-methyl-2-benzothiazolinone hydrazone assay, indicating a high hexosamine content. The substance elaborated by wild-type N. crassa can be fractionated into two components that appear by rechromatography to be closely related. When isolated from mutants of the peak (pk) locus, the corresponding polysaccharide cannot be resolved into two components. Instead, a single component is consistently found. This variant chromatographic pattern cosegregates with morphological effects of the pk allele after crosses with the wild type. The polysaccharide isolated from a wild-type culture that has been induced by sorbose to phenocopy the hyphal characteristics of pk mutants elutes from the ion exchange column in a manner similar to the corresponding polysaccharide from the pk mutants.Neurospora crassa may undergo two distinct developmental phases. One phase involves the asexual production of conidia that germinate to produce a mycelial mat producing more conidia. The second, the sexual developmental phase, consists of protoperithecia production, fertilization of the protoperithecia, and then maturation of the perithecia, or fruiting bodies. Perithecia maturation includes differentiation of the perithecial wall and internal development of asci containing ascospores. By use of appropriate mutants, loci that regulate development of one phase only (1, 2) as well as loci regulating both phases (3) have been identified. A number of loci of the second type regulate morphology of both asci and vegetative hyphae. Mutations at these various loci offer the potential for dissecting ascus development and providing insight into the relationship of the zygote to the developing ascus. Full utilization of these mutants to study development requires some knowledge of their molecular function. No consistent molecular alteration that can be directly related to alterations in morphology has been previously detected for mutations at any of these loci. The careful examination of a molecular component of Neurospora now reveals a consistent molecular alteration for members of one set of allelic mutants.Reissig and Glasgow (4) reported the isolation of a growthregulating substance both from purified hyphal wall of Neurospora and from medium that had supported a culture. They found no difference between the materials from the two sources. The substance was found to be composed primarily of 2-amino-2-deoxygalactose, with several neutral sugars and amino acids as minor components. A portion of the 2-amino-2-deoxygalactose residues was found to be N-acetylated.Originally referred to as MP (for mucopolysaccharide), the designation was later changed to galactosaminoglycan (5).Mycelial morphology in Neurospora is determined in part by the frequency and location of branching and by growth rate. The galac...

show abstract

Identification and partial characterization of a developmentally regulated protein involved in sexual morphogenesis in the discomycete plant pathogenPyrenopeziza brassicae

Ashby

1998

Physiological and Molecular Plant Pathology

View full text Add to dashboard Cite

Genetically Related Protein Variants Specifically Associated with Fruiting Body Maturation in Neurospora

Cited by 25 publications

References 8 publications

Occurrence of a major protein associated with fruiting body development in Neurospora and related Ascomycetes.

Occurrence of a major protein associated with fruiting body development in Neurospora and related Ascomycetes.

Molecular alteration in a Neurospora crassa morphological mutant and its phenocopy.

Identification and partial characterization of a developmentally regulated protein involved in sexual morphogenesis in the discomycete plant pathogenPyrenopeziza brassicae

Contact Info

Product

Resources

About