Genetically Programmed Clusters of Gold Nanoparticles for Cancer Cell-Targeted Photothermal Therapy

Oh, Mi Hwa; Yu, Jeong Heon; Kim, Insu; Nam, Yoon Sung

doi:10.1021/acsami.5b07029

Cited by 55 publications

(52 citation statements)

References 40 publications

(72 reference statements)

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“…It can facilitate construction of nanocomposites in optimum size and volume for intended use. In addition, distinct biophysical properties enable metallic NPs to be used in photothermal therapy, computed tomography, and magnetic resonance imaging, allowing them to develop as theranostic nanomedicine, as well as drug‐delivery vehicles . These beneficial features of metallic NPs may be easily adopted into our NNP system with established surface chemistry, and bioconjugation methods.…”

Section: Discussionmentioning

confidence: 99%

Programed Assembly of Nucleoprotein Nanoparticles Using DNA and Zinc Fingers for Targeted Protein Delivery

Ryu

Kang

Kim

et al. 2018

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With a growing number of intracellular drug targets and the high efficacy of protein therapeutics, the targeted delivery of active proteins with negligible toxicity is a challenging issue in the field of precision medicine. Herein, a programed assembly of nucleoprotein nanoparticles (NNPs) using DNA and zinc fingers (ZnFs) for targeted protein delivery is presented. Two types of ZnFs with different sequence specificities are genetically fused to a targeting moiety and a protein cargo, respectively. Double‐stranded DNA with multiple ZnF‐binding sequences is grafted onto inorganic nanoparticles, followed by conjugation with the ZnF‐fused proteins, generating the assembly of NNPs with a uniform size distribution and high stability. The approach enables controlled loading of a protein cargo on the NNPs, offering a high cytosolic delivery efficiency and target specificity. The utility and potential of the assembly as a versatile protein delivery vehicle is demonstrated based on their remarkable antitumor activity and target specificity with negligible toxicity in a xenograft mice model.

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Section: Discussionmentioning

confidence: 99%

Programed Assembly of Nucleoprotein Nanoparticles Using DNA and Zinc Fingers for Targeted Protein Delivery

Ryu

Kang

Kim

et al. 2018

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“…For example, Guo et al have recently shown interesting results on the PCa cells PC-3 and LNCaP indicating that two of their investigated formulations with transferrin and folate-receptor targeting ligands respectively (AuNPs-PEG-Tf and AuNPs-PEI-FA) show potential as non-viral gene delivery vectors in the treatment of PCa [141]. The photo-dynamic/thermal technique has also shown some progress recent years for both PCa and BC [156,157,158,159,160,161,162,163,164,165]. For example, Oh et al have shown promising PCa cell killing efficacy by using a 55 nm small icosahedral phage that was engineered to display a gold-binding peptide as well as a PCa cell-binding peptide and applying a 60 mW/cm 2 light irradiation [156].…”

Section: Nanoparticles For Prostate and Breast Cancermentioning

confidence: 99%

“…The photo-dynamic/thermal technique has also shown some progress recent years for both PCa and BC [156,157,158,159,160,161,162,163,164,165]. For example, Oh et al have shown promising PCa cell killing efficacy by using a 55 nm small icosahedral phage that was engineered to display a gold-binding peptide as well as a PCa cell-binding peptide and applying a 60 mW/cm 2 light irradiation [156]. Mkandawire et al have recently investigated an alternative way to treat BC cells inducing apoptosis by targeting their mitochondria using AuNPs during photothermal treatment [164].…”

Section: Nanoparticles For Prostate and Breast Cancermentioning

confidence: 99%

“…Regarding PCa and BC there are several publications investigating PTT in different NP settings [91,156,228,230,247,343,422,485,486]. For example, Hosoya et al evaluated a theranostic hydrogel-based NP platform combining both targeting of the tumor cells, photon-to-heat conversion, as well as triggered drug delivery enabling controlled release of the anticancer drug and multimodal imaging [247].…”

Section: Multimodal Therapy Options For Prostate and Breast Cancermentioning

confidence: 99%

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Nanoparticles as Theranostic Vehicles in Experimental and Clinical Applications—Focus on Prostate and Breast Cancer

Elgqvist

2017

IJMS

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Prostate and breast cancer are the second most and most commonly diagnosed cancer in men and women worldwide, respectively. The American Cancer Society estimates that during 2016 in the USA around 430,000 individuals were diagnosed with one of these two types of cancers, and approximately 15% of them will die from the disease. In Europe, the rate of incidences and deaths are similar to those in the USA. Several different more or less successful diagnostic and therapeutic approaches have been developed and evaluated in order to tackle this issue and thereby decrease the death rates. By using nanoparticles as vehicles carrying both diagnostic and therapeutic molecular entities, individualized targeted theranostic nanomedicine has emerged as a promising option to increase the sensitivity and the specificity during diagnosis, as well as the likelihood of survival or prolonged survival after therapy. This article presents and discusses important and promising different kinds of nanoparticles, as well as imaging and therapy options, suitable for theranostic applications. The presentation of different nanoparticles and theranostic applications is quite general, but there is a special focus on prostate cancer. Some references and aspects regarding breast cancer are however also presented and discussed. Finally, the prostate cancer case is presented in more detail regarding diagnosis, staging, recurrence, metastases, and treatment options available today, followed by possible ways to move forward applying theranostics for both prostate and breast cancer based on promising experiments performed until today.

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“…This ultimately amplifies the photothermal effect in the NIR region [34][35][36]. Inspired by these interesting properties, gold nanoparticle clusters (AuNCs) can be applied as an efficient photothermal agents [37][38][39][40].…”

Section: Introductionmentioning

confidence: 99%

Gold nanoparticle clusters for the investigation of therapeutic efficiency against prostate cancer under near-infrared irradiation

et al. 2020

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Gold particles have been widely used in the treatment of prostate cancer due to their unique optical properties, such as their light-heat conversion in response to near-infrared radiation. Due to well-defined synthesis mechanisms and simple manufacturing methods, gold particles have been fabricated in various sizes and shapes. However, the low photothermal transduction efficiency in their present form is a major obstacle to practical and therapeutic uses of these particles. In the current work, we present a silica-coated gold nanoparticle cluster to address the therapeutic limit of single gold nanoparticles (AuNPs) and use its photothermal effect for treatment against PC-3, a typical prostate cancer. Due to its specific nanostructure, this gold nanocluster showed three times higher photothermal transduction efficiency than free single AuNPs. Moreover, while free single particles easily clump and lose optical properties, this silica-coated cluster form remained stable for a longer time in a given medium. In photothermal tests under near-infrared radiation, the excellent therapeutic efficacy of gold nanoclusters, referred to as AuNC@SiO 2 , was observed in a preclinical sample. Only the samples with both injected nanoclusters followed by photothermal treatment showed completely degraded tumors after 15 days. Due to the unique intrinsic biocompatibility and higher therapeutic effect of these silica-coated gold nanoclusters, they may contribute to enhancement of therapeutic efficacy against prostate cancer.

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Genetically Programmed Clusters of Gold Nanoparticles for Cancer Cell-Targeted Photothermal Therapy

Cited by 55 publications

References 40 publications

Programed Assembly of Nucleoprotein Nanoparticles Using DNA and Zinc Fingers for Targeted Protein Delivery

Programed Assembly of Nucleoprotein Nanoparticles Using DNA and Zinc Fingers for Targeted Protein Delivery

Nanoparticles as Theranostic Vehicles in Experimental and Clinical Applications—Focus on Prostate and Breast Cancer

Gold nanoparticle clusters for the investigation of therapeutic efficiency against prostate cancer under near-infrared irradiation

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