Genetically modified cell sheets in regenerative medicine and tissue engineering

Jiang, Zhiwei; Li, Na; Zhu, Danji; Ren, Lingfei; Shao, Qing; Yu, Ke; Yang, Guoli

doi:10.1016/j.biomaterials.2021.120908

Cited by 23 publications

(20 citation statements)

References 169 publications

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“…However, gaining bone regeneration at large bone defects parts is still challenging. Therefore, recently, genes and/or RNAi modified osteoinductive artificial scaffolds (Laird et al, 2021;Sun et al, 2021) and genetically arranged high functional cell seeded scaffolds (Sato et al, 2018;Gugliandolo et al, 2021;Jiang et al, 2021) have been considered as new upcoming bone regeneration strategies.…”

Section: Discussionmentioning

confidence: 99%

Osteogenic Response to Polysaccharide Nanogel Sheets of Human Fibroblasts After Conversion Into Functional Osteoblasts by Direct Phenotypic Cell Reprogramming

Nakai

Yamamoto

Kishida

et al. 2021

Front. Bioeng. Biotechnol.

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Human dermal fibroblasts (HDFs) were converted into osteoblasts using a ALK inhibitor II (inhibitor of transforming growth factor-β signal) on freeze-dried nanogel-cross-linked porous (FD-NanoClip) polysaccharide sheets or fibers. Then, the ability of these directly converted osteoblasts (dOBs) to produce calcified substrates and the expression of osteoblast genes were analyzed in comparison with osteoblasts converted by exactly the same procedure but seeded onto a conventional atelocollagen scaffold. dOBs exposed to FD-NanoClip in both sheet and fiber morphologies produced a significantly higher concentration of calcium deposits as compared to a control cell sample (i.e., unconverted fibroblasts), while there was no statistically significant difference in calcification level between dOBs exposed to atelocollagen sheets and the control group. The observed differences in osteogenic behaviors were interpreted according to Raman spectroscopic analyses comparing different polysaccharide scaffolds and Fourier transform infrared spectroscopy analyses of dOB cultures. This study substantiates a possible new path to repair large bone defects through a simplified transplantation procedure using FD-NanoClip sheets with better osteogenic outputs as compared to the existing atelocollagen scaffolding material.

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Section: Discussionmentioning

confidence: 99%

Osteogenic Response to Polysaccharide Nanogel Sheets of Human Fibroblasts After Conversion Into Functional Osteoblasts by Direct Phenotypic Cell Reprogramming

Nakai

Yamamoto

Kishida

et al. 2021

Front. Bioeng. Biotechnol.

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“…In recent years, genetically modified cell sheets using virus-based or non-viral gene transfection have shown great potential in personalized and precision medicine [ 188 ]. Sesns-modified cell sheets might act as a promising preventive or therapeutic drug against aging-related diseases such as sarcopenia, cancers, AD, PD, and CAD.…”

Section: Future Directions: Sesns As Biomarkers and Therapeutic Targets Of Diseasesmentioning

confidence: 99%

The functions and roles of sestrins in regulating human diseases

Chen

Huang

et al. 2022

Cell Mol Biol Lett

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Sestrins (Sesns), highly conserved stress-inducible metabolic proteins, are known to protect organisms against various noxious stimuli including DNA damage, oxidative stress, starvation, endoplasmic reticulum (ER) stress, and hypoxia. Sesns regulate metabolism mainly through activation of the key energy sensor AMP-dependent protein kinase (AMPK) and inhibition of mammalian target of rapamycin complex 1 (mTORC1). Sesns also play pivotal roles in autophagy activation and apoptosis inhibition in normal cells, while conversely promoting apoptosis in cancer cells. The functions of Sesns in diseases such as metabolic disorders, neurodegenerative diseases, cardiovascular diseases, and cancer have been broadly investigated in the past decades. However, there is a limited number of reviews that have summarized the functions of Sesns in the pathophysiological processes of human diseases, especially musculoskeletal system diseases. One aim of this review is to discuss the biological functions of Sesns in the pathophysiological process and phenotype of diseases. More significantly, we include some new evidence about the musculoskeletal system. Another purpose is to explore whether Sesns could be potential biomarkers or targets in the future diagnostic and therapeutic process.

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“…Bone regeneration is a more complicated process than osteogenesis and physical function recovery. With the constant development of the PDLSC sheets for promoting bone formation, genetic modification may become another promising method at the same time [ 73 ]. In short, how to better improve the function of the sheets is still a problem worthy of in-depth study.…”

Section: Pdlsc Sheets Applicationsmentioning

confidence: 99%

Dental-derived mesenchymal stem cell sheets: a prospective tissue engineering for regenerative medicine

Chen

Huang

et al. 2022

Stem Cell Res Ther

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Stem cells transplantation is the main method of tissue engineering regeneration treatment, the viability and therapeutic efficiency are limited. Scaffold materials also play an important role in tissue engineering, whereas there are still many limitations, such as rejection and toxic side effects caused by scaffold materials. Cell sheet engineering is a scaffold-free tissue technology, which avoids the side effects of traditional scaffolds and maximizes the function of stem cells. It is increasingly being used in the field of tissue regenerative medicine. Dental-derived mesenchymal stem cells (DMSCs) are multipotent cells that exist in various dental tissues and can be used in stem cell-based therapy, which is impactful in regenerative medicine. Emerging evidences show that cell sheets derived from DMSCs have better effects in the field of regenerative medicine applications. Extracellular matrix (ECM) is the main component of cell sheets, which is a dynamic repository of signalling biological molecules and has a variety of biological functions and may play an important role in the application of cell sheets. In this review, we summarized the application status, mechanisms that sheets and ECM may play and future prospect of DMSC sheets on regeneration medicine.

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Genetically modified cell sheets in regenerative medicine and tissue engineering

Cited by 23 publications

References 169 publications

Osteogenic Response to Polysaccharide Nanogel Sheets of Human Fibroblasts After Conversion Into Functional Osteoblasts by Direct Phenotypic Cell Reprogramming

Osteogenic Response to Polysaccharide Nanogel Sheets of Human Fibroblasts After Conversion Into Functional Osteoblasts by Direct Phenotypic Cell Reprogramming

The functions and roles of sestrins in regulating human diseases

Dental-derived mesenchymal stem cell sheets: a prospective tissue engineering for regenerative medicine

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