Genetically modified canine Schwann cells—In vitro and in vivo evaluation of their suitability for peripheral nerve tissue engineering

Schmitte, Ruth; Tipold, Andrea; Stein, Veronika M.; Schenk, Henning; Flieshardt, C.; Grothe, Claudia; Haastert, Kirsten

doi:10.1016/j.jneumeth.2009.11.023

Cited by 26 publications

(22 citation statements)

References 19 publications

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“…Schwann cell transplantation enhances axon outgrowth both in vitro ( Schlosshauer et al, 2003) and in vivo ( Keilhoff et al, 2006). Transplantation of viable SCs offers better results than the sheer release of growth factors, because SCs have the above mentioned regeneration promoting effect ( Bhatheja and Field, 2006;Fansa et al, 1999;Hall, 1978;Ide, 1996;Madduri and Gander, 2010;Muir, 2010;Schmitte et al, 2010). The generation of sufficient amounts of SCs for auto-transplantation, however, requires a significant time for cell culture.…”

Section: Introductionmentioning

confidence: 99%

Use of poly(DL-lactide-ε-caprolactone) membranes and mesenchymal stem cells from the Wharton's jelly of the umbilical cord for promoting nerve regeneration in axonotmesis: In vitro and in vivo analysis

et al. 2012

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Cellular systems implanted into an injured nerve may produce growth factors or extracellular matrix molecules, modulate the inflammatory process and eventually improve nerve regeneration. In the present study, we evaluated the therapeutic value of human umbilical cord matrix MSCs (HMSCs) on rat sciatic nerve after axonotmesis injury associated to Vivosorbs membrane. During HMSCs expansion and differentiation in neuroglial-like cells, the culture medium was collected at 48, 72 and 96 h for nuclear magnetic resonance (NMR) analysis in order to evaluate the metabolic profile. To correlate the HMSCs ability to differentiate and survival capacity in the presence of the Vivosorbs membrane, the [Ca 2þ ]i of undifferentiated HMSCs or neuroglial-differentiated HMSCs was determined by the epifluorescence technique using the Fura-2AM probe. The Vivosorbs membrane proved to be adequate and used as scaffold associated with undiffer-entiated HMSCs or neuroglial-differentiated HMSCs. In vivo testing was carried out in adult rats where a sciatic nerve axonotmesis injury was treated with undifferentiated HMSCs or neuroglial differentiated HMSCs with or without the Vivosorbs membrane. Motor and sensory functional recovery was evaluated throughout a healing period of 12 weeks using sciatic functional index (SFI), extensor postural thrust (EPT), and withdrawal reflex latency (WRL).Stereological analysis was carried out on regenerated nerve fibers. In vitro investigation showed the formation of typical neuroglial cells after differentiation, which were positively stained for the typical specific neuroglial markers such as the GFAP, the GAP-43 and NeuN.NMR showed clear evidence that HMSCs expansion is glycolysis-dependent but their differentiation requires the switch of the metabolic profile to oxidative metabolism. In vivo studies showed enhanced recovery of motor and sensory function in animals treated with transplanted undifferentiated and differentiated HMSCs that was accompanied by an increase in myelin sheath. Taken together, HMSC from the umbilical cord Wharton jelly might be useful for improving the clinical outcome after peripheral nerve lesion.

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Section: Introductionmentioning

confidence: 99%

Use of poly(DL-lactide-ε-caprolactone) membranes and mesenchymal stem cells from the Wharton's jelly of the umbilical cord for promoting nerve regeneration in axonotmesis: In vitro and in vivo analysis

et al. 2012

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“…For chitosan core implantation (CES), the epifascicular epi-/perineurium was cut in a longitudinal direction and the fascicles were resected over a distance of 10 mm leaving an empty epineural sleeve [25, 26]. The chitosan core was then implanted and the epifascicular epi-/perineurium was closed using single interrupted 9-0 Ethilon sutures.…”

Section: Methodsmentioning

confidence: 99%

Outer Electrospun Polycaprolactone Shell Induces Massive Foreign Body Reaction and Impairs Axonal Regeneration through 3D Multichannel Chitosan Nerve Guides

Duda

Dreyer

Behrens

et al. 2014

BioMed Research International

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We report on the performance of composite nerve grafts with an inner 3D multichannel porous chitosan core and an outer electrospun polycaprolactone shell. The inner chitosan core provided multiple guidance channels for regrowing axons. To analyze the in vivo properties of the bare chitosan cores, we separately implanted them into an epineural sheath. The effects of both graft types on structural and functional regeneration across a 10 mm rat sciatic nerve gap were compared to autologous nerve transplantation (ANT). The mechanical biomaterial properties and the immunological impact of the grafts were assessed with histological techniques before and after transplantation in vivo. Furthermore during a 13-week examination period functional tests and electrophysiological recordings were performed and supplemented by nerve morphometry. The sheathing of the chitosan core with a polycaprolactone shell induced massive foreign body reaction and impairment of nerve regeneration. Although the isolated novel chitosan core did allow regeneration of axons in a similar size distribution as the ANT, the ANT was superior in terms of functional regeneration. We conclude that an outer polycaprolactone shell should not be used for the purpose of bioartificial nerve grafting, while 3D multichannel porous chitosan cores could be candidate scaffolds for structured nerve grafts.

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“…If SC-derived vesicles are demonstrated to have functional roles in axonal regeneration, SC differentiation, or other processes crucial for neural tissue regeneration, these vesicles can be used for therapeutic purposes by using their endogenous potentials or by loading them with specific transcript or proteins by modifying glial cells, which can be easily manipulated in vitro (Schmitte et al, 2010; Zhang et al, 2010). It has been demonstrated that neuronal-targeted exosomes obtained from genetically modified dendritic cells in vitro can be electroporated with specific siRNA, and after intravenous injection, they specifically knock-down their target gene in brain neurons (Alvarez-Erviti et al, 2011).…”

Section: Potential Roles and Functions Of Secreted Vesicles In The Pnsmentioning

confidence: 99%

Transfer of Vesicles From Schwann Cells to Axons: a Novel Mechanism of Communication in the Peripheral Nervous System

Lopez-Verrilli¹,

Court²

2012

Front. Physio.

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Schwann cells (SCs) are the glial component of the peripheral nervous system, with essential roles during development and maintenance of axons, as well as during regenerative processes after nerve injury. SCs increase conduction velocities by myelinating axons, regulate synaptic activity at presynaptic nerve terminals and are a source of trophic factors to neurons. Thus, development and maintenance of peripheral nerves are crucially dependent on local signaling between SCs and axons. In addition to the classic mechanisms of intercellular signaling, the possibility of communication through secreted vesicles has been poorly explored to date. Interesting recent findings suggest the occurrence of lateral transfer mediated by vesicles from glial cells to axons that could have important roles in axonal growth and axonal regeneration. Here, we review the role of vesicular transfer from SCs to axons and propose the advantages of this means in supporting neuronal and axonal maintenance and regeneration after nerve damage.

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Genetically modified canine Schwann cells—In vitro and in vivo evaluation of their suitability for peripheral nerve tissue engineering

Cited by 26 publications

References 19 publications

Use of poly(DL-lactide-ε-caprolactone) membranes and mesenchymal stem cells from the Wharton's jelly of the umbilical cord for promoting nerve regeneration in axonotmesis: In vitro and in vivo analysis

Use of poly(DL-lactide-ε-caprolactone) membranes and mesenchymal stem cells from the Wharton's jelly of the umbilical cord for promoting nerve regeneration in axonotmesis: In vitro and in vivo analysis

Outer Electrospun Polycaprolactone Shell Induces Massive Foreign Body Reaction and Impairs Axonal Regeneration through 3D Multichannel Chitosan Nerve Guides

Transfer of Vesicles From Schwann Cells to Axons: a Novel Mechanism of Communication in the Peripheral Nervous System

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