Genetically Epilepsy‐Prone Rats are Characterized by Altered Tissue Trace Element Concentrations

Carl, G. F.; Critchfield, James W.; Thompson, Jennifer L.; Holmes, Gregory L.; Gallagher, Brian B.; Keen, Carl L.

doi:10.1111/j.1528-1157.1990.tb05372.x

Cited by 26 publications

(8 citation statements)

References 22 publications

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“…It is understood that seizures produce unusually sharp fluctuations in the internal environment of an organism (Carl et al, 1990). Maintenance of acidic internal environments (i.e.…”

Section: Discussionmentioning

confidence: 99%

Herbal treatment following post‐seizure induction in rat by lithium pilocarpine: Scutellaria lateriﬂora (Skullcap), Gelsemium sempervirens (Gelsemium) and Datura stramonium (Jimson Weed) may prevent development of spontaneous seizures

Peredery

Persinger

2004

Phytotherapy Research

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About 1 week after the induction of status epilepticus in male rats by a single systemic injection of lithium (3 mEq/kg) and pilocarpine (30 g/kg), rats were continuously administered one of three herbal treatments through the water supply for 30 days. A fourth group received colloidal minerals and diluted food grade hydrogen peroxide in tap water, while a fifth group of rats received only tap water (control). Herbal treatments were selected for their historical antiseizure activities and sedative actions on the nervous system. The numbers of spontaneous seizures per day during a 15 min observation interval were recorded for each rat during the treatment period and during an additional 30 days when only tap water was given. Rats that received a weak solution of the three herbal fluid extracts of Scutellaria lateri flora (Skullcap), Gelsemium sempervirens (Gelsemium) and Datura stramonium (Jimson Weed) displayed no seizures during treatment while all the other groups were not seizure-free. However, when this treatment was removed, the rats in this group displayed numbers of spontaneous seizures comparable to the controls. Although there is no proof that herbal remedies can control limbic or temporal lobe epilepsy, the results of this experiment strongly suggest that the appropriate combination of herbal compounds may be helpful as adjunctive interventions.

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“…It is understood that seizures produce unusually sharp fluctuations in the internal environment of an organism (Carl et al, 1990). Maintenance of acidic internal environments (i.e.…”

Section: Discussionmentioning

confidence: 99%

Herbal treatment following post‐seizure induction in rat by lithium pilocarpine: Scutellaria lateriﬂora (Skullcap), Gelsemium sempervirens (Gelsemium) and Datura stramonium (Jimson Weed) may prevent development of spontaneous seizures

Peredery

Persinger

2004

Phytotherapy Research

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“…Hence, these elements are responsible for various metabolic processes, including those occurring in the brain (16). Existing evidence suggests that epileptic seizures signifi cantly change the metabolism and distribution of trace elements in the nervous tissue (17)(18)(19). On the other hand, metal contents determine susceptibility to convulsions (20).…”

Section: Discussionmentioning

confidence: 99%

Major and minor bio-element status in children with febrile seizure

Akbayram¹,

Cemek²,

Büyükben³

et al. 2012

BLL

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Febrile seizures (FS) are the most common cause of seizures in children. The exact etiopathogenesis is unknown but involves factors like genetic predisposition and alterations in the levels of neurotransmitters and some trace elements. The study includes 48 consecutive children with FS, and 55 healthy age matched control subjects. Calcium, magnesium and potassium concentrations in the febrile study group were lower than in the control group (p<0.05). Iron and Gallium levels in the study group were lower than in the control group (p<0.01). Serum Selenium (p<0.001), Zinc (p<0.001) and Strontium (p<0.05) levels were signifi cantly decreased in the study group when compared to the control group. There was no signifi cant difference between the control and study groups in Serum Barium, Beryllium and Copper levels (p>0.05). The aim of the present prospective analytical case-control study was to determine whether there was any change in element levels in children with FS (Ref. 33). Full Text in PDF www.elis.sk.

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“…Existing evidence suggests that epileptic seizures significantly change metabolism and distribution of trace elements in the nervous tissue [6][7][8]. From the other hand, content of metals determinates susceptibility to convulsions [9].…”

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confidence: 99%

The role of trace elements in the pathogenesis and progress of pilocarpine-induced epileptic seizures

et al. 2008

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Epilepsy, the third most common neurological disorder, is characterized by recurrent unprovoked seizures. Although the mechanisms of epileptic convulsions have been a subject of intensive investigations for many years in most cases the etiology of the disease is not known [1].Metallic elements participating in the processes like: oxidative stress, excitotoxicity, mitochondrial dysfunction or protein aggregation may lead to the atrophy and death of neurons in case of some neurodegenerative disorders [2][3][4]. Because epileptic seizures induce neurodegeneration in selected areas of brain it is suspected that metals may be involved in the pathogenesis and progress of epilepsy as well [5].Existing evidence suggests that epileptic seizures significantly change metabolism and distribution of trace elements in the nervous tissue [6][7][8]. From the other hand, content of metals determinates susceptibility to convulsions [9].In our experiment we used pilocarpine in order to induce epilepsy in rats. In order to find what elements are involved in the pathogenesis and progress of epileptic convulsions the comparison between pilocarpine treated and control rats was done. The samples of rat brain were cut using a cryomicrotome into 15 µm thick slices. Thin tissue sections were mounted on Ultralene foil and freeze-dried.X-ray fluorescence microscopy was employed for topographic and quantitative analysis of selected metals in the areas of brain tissue involved in epilepsy. The measurements were done on the bending magnet beamline L at HASYLAB. The multilayer monochromator was applied and the primary photon energy was set to 17 keV. The beam was focused with polycapillary optics for a final beam spot dimension of 15 µm x 15 µm. The characteristic X-ray lines were measured using the Vortex SDD detector from SII Nano Technology USA inc. and the time of spectra acquisition was equal to 6 s per pixel. Measurements of NIST standard reference materials (SRM 1833 and SRM 1832) were performed for spectrometer calibration.The elements such us P, S, Cl, K, Ca, Fe, Cu, Zn, Br, Rb and Sr were detected in rat brain sections. For each sample two-dimensional analysis of elemental distribution was performed for the area of hippocampus and brain cortex. The results of such analysis for selected control and epileptic sample were shown in Figures 1 and 2 respectively.For four regions of hippacampus formation (CA1 -Cornu Ammonis, CA3, DG -Dentate Gyrus and H -Hilus) and motor cortex the mean masses per unit area were evaluated. The areas taken into account in calculations were equal to 300 µm per 300 µm. The preliminary results showed that the level of Ca tends to be higher for CA1 region of hippocampus and cortex in case of animals with pilocarpine induced epilepsy. The opposite relation was observed for Zn level in the area of DG.1551

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Genetically Epilepsy‐Prone Rats are Characterized by Altered Tissue Trace Element Concentrations

Cited by 26 publications

References 22 publications

Herbal treatment following post‐seizure induction in rat by lithium pilocarpine: Scutellaria lateriﬂora (Skullcap), Gelsemium sempervirens (Gelsemium) and Datura stramonium (Jimson Weed) may prevent development of spontaneous seizures

Herbal treatment following post‐seizure induction in rat by lithium pilocarpine: Scutellaria lateriﬂora (Skullcap), Gelsemium sempervirens (Gelsemium) and Datura stramonium (Jimson Weed) may prevent development of spontaneous seizures

Major and minor bio-element status in children with febrile seizure

The role of trace elements in the pathogenesis and progress of pilocarpine-induced epileptic seizures

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