Genetically-enriched microbe-facilitated self-healing concrete – a sustainable material for a new generation of construction technology

Sarkar, Manas; Adak, Dibyendu; Tamang, Abiral; Chattopadhyay, Brajadulal; Mandal, Saroj

doi:10.1039/c5ra20858k

Cited by 61 publications

(30 citation statements)

References 19 publications

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“…The characteristics absorption spectrum of the synthesized nanoparticles recorded by spectrophotometer (Figure 6) was a clear evidence of gold nanoparticles where absorption maximum of GNPs was appeared at 523 nm as reported earlier [17]. Our earlier works have shown that one hot spring bacteria isolated from Bakreshwar, West Bengal are able to increase the compressive strength of concrete material when incorporated [10] [11] [18] as well as to synthesis silica nanoparticles from silicate substrate. The bacterium BKH1 is found to secrete a protein (Bioremediase; UniProt Knowledgebase under the accession no.…”

Section: Resultsmentioning

confidence: 82%

“…Samples were then air dried and transferred onto a FESEM alumina supports and sputtered with gold by a coater of Blazers. Slides were observed under FESEM (INSPECT F50 SEM, FEI Europe BV, and Netherlands) and photomicrographs of the bacterial cells were taken in single (40×) magnification [10].…”

Section: Morphology Study By Fesemmentioning

confidence: 99%

“…The sequence similarity was searched on this using BLAST. Phylogenetic analysis of the isolated strain was done by neighbour-joining method where distances between sequences were determined by Kimura's two-parameter model [10] [14]. The confidence limits of the branching were performed by Bootstrap analysis [15].…”

Section: Phylogenetic Analysismentioning

confidence: 99%

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Characterization of a Novel MDH1 Bacterium from a Virgin Hot Spring Applicable for Gold Nanoparticle (GNPs) Synthesis

Alam¹,

Sarkar²,

Chowdhury³

et al. 2016

AiM

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Background: Use of novel microorganisms for beneficial purposes is still remaining a challenging job. This study was designed to isolate, characterize and use of a novel hot spring bacterial strain from a virgin hot spring of Metaldanga, Birbhum, West Bengal, India. Methods: A pure bacterial strain (MDH1) was identified by growing the enrichment culture isolated from Metaldanga hot spring through serial dilution process in a semi-synthetic medium at pH 8.0 and 42˚C temperature. The novelty of the strain was characterized by 16S-rRNA gene sequence analysis. The bacterium acted as template to synthesize spherical gold nanoparticles (GNPs). GNPs were characterized by using UV-Vis spectroscopy, X-ray diffraction (XRD), dynamic light scattering (DLS) and Fourier transform infra-red spectroscopy (FTIR). Results: The phylogenetic analysis suggested that MDH1 strain (GenBank accession number: KT600031) was affiliated to the family "Pseudomonadaceae" with 99% homologous to Pseudomonas putida H8234. The coccoid shaped bacterium was gramnegative and facultative-anaerobic which acted as a template to synthesize spherical GNPs with an average size of 12 ± 3 nm when examined under transmission electron microscopy (TEM). FT-IR studies revealed the presence of bioactive functional groups which acted as capping and stabilizing agents of the GNPs. XRD pattern confirmed the amorphous nature of GNPs. The Zeta potential (ζ) concluded the adequate stability of GNPs in an aqueous environment. Conclusions: The present investigation explores the microbial diversity of a virgin hot spring of Metaldanga for its beneficial applications in industry, particularly in the synthesis of the gold nanoparticles.

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Section: Resultsmentioning

confidence: 82%

Section: Morphology Study By Fesemmentioning

confidence: 99%

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Characterization of a Novel MDH1 Bacterium from a Virgin Hot Spring Applicable for Gold Nanoparticle (GNPs) Synthesis

Alam¹,

Sarkar²,

Chowdhury³

et al. 2016

AiM

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“…have been isolated from Bakreshwar hot springs previously, but none of them were found to be highly alkaliphilic as reported earlier (Sarkar et al . , ; Chaudhuri et al . ).…”

Section: Discussionmentioning

confidence: 99%

“…; Sarkar et al . , ). Unfortunately all of those isolated hot spring strains grow at pH 8·0 and survive only for 10–15 days within the cementitious matrices owing to very high alkaline environment.…”

Section: Introductionmentioning

confidence: 99%

An alkaliphilic bacterium BKH 4 of Bakreshwar hot spring pertinent to bioconcrete technology

et al. 2019

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Aims Hot springs have always drawn attention due to their unique chemical richness and the presence of different microbial communities. The use of hot spring bacteria in concrete technology is our primary focus; isolation of an alkaliphilic bacterium from the Bakreshwar hot springs having longer survival and better efficacy towards cementitious environment was the basis of our study's design. Methods and Results A novel facultative anaerobic and highly alkaliphilic bacterial strain (BKH4; GenBank accession no. ) belonging to the family ‘Bacillaceae’ and homologous (99%) with Lysinibacillus fusiformis was isolated from Bakreshwar hot springs. The isolated coccoid‐type Gram‐positive bacterium grows well in a defined semi‐synthetic medium (pH 12·0 and 65°C). This bacterium survives for more than a month and shows better efficacy in enhancing compressive strengths (>50%), ultrasonic pulse velocity (>25%) and durability of the cementitious mortar when incorporated at a concentration of 104 cells per ml of water used. Conclusion The novel bacterium BKH4 is more effective for the enhancement of the bioconcrete properties. Significance and Impact of the Study BKH4 bacterium will add a new dimension to future concrete technology for its usefulness in strength enhancement and durability due to its alkaliphilic nature and longer survival within a cementitious environment.

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Self‐Healing Bacterial Cementitious Composites

Preetham

Krishna

Chandraprabha

et al. 2021

Self‐Healing Smart Materials and Allied Applications

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Genetically-enriched microbe-facilitated self-healing concrete – a sustainable material for a new generation of construction technology

Abstract: Genetically modified spore forming B. subtilis bacterial cells for eco-friendly sustainable self-healing bio-concrete.

Cited by 61 publications

References 19 publications

Characterization of a Novel MDH1 Bacterium from a Virgin Hot Spring Applicable for Gold Nanoparticle (GNPs) Synthesis

Characterization of a Novel MDH1 Bacterium from a Virgin Hot Spring Applicable for Gold Nanoparticle (GNPs) Synthesis

An alkaliphilic bacterium BKH 4 of Bakreshwar hot spring pertinent to bioconcrete technology

Self‐Healing Bacterial Cementitious Composites

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