Genetically Engineered Resistance to Fusarium Head Blight in Wheat by Expression of <i>Arabidopsis NPR1</i>

Makandar, Ragiba; Essig, Juliane S; Schapaugh, Melissa A; Trick, Harold N.; Shah, Jyoti

doi:10.1094/mpmi-19-0123

Cited by 311 publications

(256 citation statements)

References 41 publications

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“…Results obtained in this study confirm that several defense-related genes are induced at higher levels in the AtNPR1 transformants compared to their non-transgenic counterparts. Similar results, showing higher-level induction of downstream defense genes, have been obtained by others in wheat and apple (Makandar et al 2006;Chen et al 2012). It is interesting that the WT plants show little, if any, induction of these defense-related genes.…”

Section: Discussionsupporting

confidence: 88%

“…Similar limitations were observed in the AtNPR1 transformants against Verticillium wilt, where protection was observed only against the non-defoliating isolates of V. dahliae and not the defoliating isolates (Parkhi et al 2010b). Nevertheless, there are a large number of reports that show that NPR1 overexpression confers a strong protection against a variety of diseases (Lin et al 2004;Makandar et al 2006;Malnoy et al 2007;Wally et al 2009;Zhang et al 2010;Dutt et al 2015;Silva et al 2015). Thus, controlled expression of NPR1 gene may still prove to be a useful means to confer tolerance to some pathogens in the field.…”

Section: Discussionmentioning

confidence: 58%

“…Transgenic expression of NPR1 gene in various plant species has been shown to confer tolerance to a broad spectrum of diseases. Makandar et al (2006) showed that the expression of Arabidopsis NPR1 gene in susceptible wheat cultivar conferred resistance to Fusarium head blight (FHB) caused by F. graminearum. They reported that this resistance against FHB is associated with faster activation of defense response in AtNPR1-expressing transgenic wheat.…”

Section: Introductionmentioning

confidence: 99%

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Response of AtNPR1-expressing cotton plants to Fusarium oxysporum f. sp. vasinfectum isolates

Joshi

Kumar

Janga

et al. 2017

Physiol Mol Biol Plants

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In our earlier investigation, we had demonstrated that transgenic cotton plants expressing AtNPR1 showed significant tolerance to Fusarium oxysporum f. sp. vasinfectum, isolate 11 (Fov11) and several other pathogens. The current study was designed to further characterize the nature of the protection provided by AtNPR1 expression and its limitations. Green Fluorescent Protein-expressing Fov11 was generated and used to study the progression of the disease within the plant. The results show that the spread of the pathogen was slower in the AtNPR1-transformants compared to the wild type plants. Transcript analysis in the seedling root and hypocotyl showed that the transgenic lines are capable of launching a stronger defense response when infected with Fov11. We further confirmed that AtNPR1 transformants showed greater degree of tolerance to Fov11. However, little or no protection was observed against a related, but more virulent isolate, Fov43, and a highly virulent isolate, CA9.

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Section: Discussionsupporting

confidence: 88%

Section: Discussionmentioning

confidence: 58%

Section: Introductionmentioning

confidence: 99%

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Response of AtNPR1-expressing cotton plants to Fusarium oxysporum f. sp. vasinfectum isolates

Joshi

Kumar

Janga

et al. 2017

Physiol Mol Biol Plants

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“…For example, the NPR1 gene is a central regulator in both monocots and dicots. The NPR1 gene was successfully used in several plant species like Arabidopsis (Cao et al 1998), rice (Chern et al 2001), tomato (Ekengren et al 2003), and wheat (Makandar et al 2006). Similarly, the mlo gene functions in barley (Peterhänsel and Lahaye 2005), tomato (Bai et al 2008), and Arabidopsis (Consonni et al 2006).…”

Section: Introductionmentioning

confidence: 99%

Potential Candidate Genes for Improving Rice Disease Resistance

Delteil

Zhang²,

Lessard³

et al. 2010

Rice

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Diseases caused by fungal and bacterial pathogens like Magnaporthe oryzae and Xanthomonas oryzae pv. oryzae are responsible for considerable yield loss. Up to now, in rice, the modification of the expression of more than 60 genes from diverse origins has shown beneficial effects with respect to disease resistance. In this paper, we review this large set of data to identify the best genes and strategies to achieve disease resistance by transgenic approaches. Altered expression of genes involved in signal transduction and transcription may lead to many unwanted side effects, like lesion mimic phenotypes. Moreover, modification of resistance to abiotic stress has been neglected and should be carefully examined in the future. Genes like resistance genes and pathogenesis-related genes can confer broad spectrum and high levels of resistance to several pathogens. Preformed expression of defense is often observed but does not necessarily lead to detrimental effects. Although examples of gene pyramiding are scarce, they suggest that this is a very promising strategy. More field evaluation of the transgenic plants is required to draw final conclusions on the usefulness of these genes for improving disease resistance.

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“…Of these, 3 were sterile and 8 were silenced in later generations. Consequently the final transformation efficiency of transcriptionally active transgenic Alvarez et al (2000), Anand et al (2003), Makandar et al (2006) and Chen et al (2008), who also observed silencing during the early generations of selfing in transgenic plants. Gene silencing is usually associated with integration of transgenes in multiple copies and to sequence homology between a transgene and an endogenous gene.…”

Section: Discussionmentioning

confidence: 95%

Increased tolerance to wheat powdery mildew by heterologous constitutive expression of the Solanum chacoense Snakin-1 gene

Faccio¹,

Rovere²,

Hopp³

et al. 2011

CZECH J GENET PLANT

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Great efforts are currently being devoted to studying the use of transgenes to confer resistance to phytopathogenic fungi. Snakin-1 is a broad-spectrum antimicrobial peptide isolated from Solanum that is active in vitro against bacteria and fungi. Recently, it was reported that overexpression of the snakin-1 (SN1) gene in transgenic potato plants enhanced resistance to Rhizoctonia solani and Erwinia carotovora. In this work wheat transgenic plants that constitutively expressed the S. chacoense SN1 gene were challenged with Blumeria graminis f.sp. tritici. Enhanced resistance to the pathogen was observed in two transgenic lines in which the development of the disease was delayed and reduced compared with the wild type variety ProINTA Federal. An association between high resistance to the pathogen and a high level of snakin-1 transcripts in the plant was observed. This is the first report on SN1 gene expression in Gramineae and its effects on wheat powdery mildew development.

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Genetically Engineered Resistance to Fusarium Head Blight in Wheat by Expression of Arabidopsis NPR1

Cited by 311 publications

References 41 publications

Response of AtNPR1-expressing cotton plants to Fusarium oxysporum f. sp. vasinfectum isolates

Response of AtNPR1-expressing cotton plants to Fusarium oxysporum f. sp. vasinfectum isolates

Potential Candidate Genes for Improving Rice Disease Resistance

Increased tolerance to wheat powdery mildew by heterologous constitutive expression of the Solanum chacoense Snakin-1 gene

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