Genetically encoded tools for measuring and manipulating metabolism

Choe, Mangyu; Titov, Denis V.

doi:10.1038/s41589-022-01012-8

Cited by 24 publications

(3 citation statements)

References 100 publications

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“…We investigated the changes in cellular ATP and the main pathways involved using ATeam1.03 YEMK (Choe & Titov, 2022) expressed in neurons or astrocytes (Lerchundi et al., 2019; Lerchundi et al., 2020). Our experiments demonstrate that chemical ischemia induces a rapid decrease in ATP levels of neurons and astrocytes, confirming earlier observations (e.g.…”

Section: Discussionmentioning

confidence: 99%

Activation of TRPV4 channels promotes the loss of cellular ATP in organotypic slices of the mouse neocortex exposed to chemical ischemia

Pape

Rose

2023

The Journal of Physiology

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Section: Discussionmentioning

confidence: 99%

Activation of TRPV4 channels promotes the loss of cellular ATP in organotypic slices of the mouse neocortex exposed to chemical ischemia

Pape

Rose

2023

The Journal of Physiology

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“…Most biomedical companies maintain great spectrophotometers for quantitative measurement in diverse research. Scientists can measure the effect of metabolic intermediates on a cell by measuring the dye color in each section [7,8]. Even the degree of antigen-antibody reaction by the COVID-19 virus might be determined when it is appropriately configured [9].…”

Section: Introductionmentioning

confidence: 99%

Developing an Automated Spectrophotometer with RGB LED and Light Sensor Using Arduino Microcontroller

Cha,

Lee

2024

JIRMEPS

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This research used RGB LED (Red Green Blue Light Emission Diode), LDR (Light Dependent Resistant) sensors, 3D printed sampling cartridge, and Arduino Mega to create a spectrophotometer with automation software to select wavelength, calculate calibration parameters, and report the concentration of the materials being studied. Spectrophotometers are finding applications across many fields, including clinical laboratories, pharmaceuticals, molecular biology, biochemistry, and even the medical realm. This study assembled an Arduino mega system coupled with software for the automatic selection of best-fit color light, calibration curve, and actual concentration interpolation processes. Dilution solutions from multiple chemical materials were evaluated to examine their linearity of calibration curves. Our study demonstrated that the tested chemical samples revealed a significant regression coefficient of up to 0.9945 for serial dilution solutions. Relative concentrations were measured with remarkable precision, exhibiting less than 5.0 % error compared to a commercial spectrophotometer. These findings highlight the viability of the RGB spectrophotometer and emphasize its potential with the promise of further refinement in development.

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“…Biosensors can be easily modified to direct expression into subcellular compartments, such as the mitochondria, allowing compartment-specific metabolite analysis. We direct the reader to several comprehensive reviews on this topic that include discussions of the limitations of genetically encoded biosensors and extensive lists of biosensors available for metabolic analysis [21][22][23][24][25][26][27] . Here, we provide a partial list of potentially useful sensors in Table 1.…”

Section: Introductionmentioning

confidence: 99%

Imaging immunometabolism in situ in live animals

Molnar,

Miskolci

2024

Immunometabolism

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Immunometabolism is a rapidly developing field that holds great promise for diagnostic and therapeutic benefits to human diseases. The field has emerged based on seminal findings from in vitro and ex vivo studies that established the fundamental role of metabolism in immune cell effector functions. Currently, the field is acknowledging the necessity of investigating cellular metabolism within the natural context of biological processes. Examining cells in their native microenvironment is essential not only to reveal cell-intrinsic mechanisms but also to understand how cross-talk between neighboring cells regulates metabolism at the tissue level in a local niche. This necessity is driving innovation and advancement in multiple imaging-based technologies to enable analysis of dynamic intracellular metabolism at the single-cell level, with spatial and temporal resolution. In this review, we tally the currently available imaging-based technologies and explore the emerging methods of Raman and autofluorescence lifetime imaging microscopy, which hold significant potential and offer broad applications in the field of immunometabolism.

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Genetically encoded tools for measuring and manipulating metabolism

Cited by 24 publications

References 100 publications

Activation of TRPV4 channels promotes the loss of cellular ATP in organotypic slices of the mouse neocortex exposed to chemical ischemia

Activation of TRPV4 channels promotes the loss of cellular ATP in organotypic slices of the mouse neocortex exposed to chemical ischemia

Developing an Automated Spectrophotometer with RGB LED and Light Sensor Using Arduino Microcontroller

Imaging immunometabolism in situ in live animals

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