2005
DOI: 10.1002/cyto.a.20119
|View full text |Cite
|
Sign up to set email alerts
|

Genetically encoded sensors for metabolites

Abstract: Background: Metabolomics, i.e., the multiparallel analysis of metabolite changes occurring in a cell or an organism, has become feasible with the development of highly efficient mass spectroscopic technologies. Functional genomics as a standard tool helped to identify the function of many of the genes that encode important transporters and metabolic enzymes over the past few years. Advanced expression systems and analysis technologies made it possible to study the biochemical properties of the corresponding pr… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
1
1
1
1

Citation Types

0
36
0

Year Published

2006
2006
2015
2015

Publication Types

Select...
8
2

Relationship

0
10

Authors

Journals

citations
Cited by 54 publications
(36 citation statements)
references
References 57 publications
(58 reference statements)
0
36
0
Order By: Relevance
“…Besides genetic differences, several other sources for population heterogeneity exist, of which several are also known to cause metabolic differences. Today, methods capable of resolving differences in metabolite levels on the single cell level are provided, within limits, by molecular sensors such as FRET sensors [6,7] or aptamer-based technology [8 ,9]. Both types of molecular sensors, however, are difficult to develop, are limited to specific analytes, and quantitative analyses (e.g.…”
Section: Introductionmentioning
confidence: 99%
“…Besides genetic differences, several other sources for population heterogeneity exist, of which several are also known to cause metabolic differences. Today, methods capable of resolving differences in metabolite levels on the single cell level are provided, within limits, by molecular sensors such as FRET sensors [6,7] or aptamer-based technology [8 ,9]. Both types of molecular sensors, however, are difficult to develop, are limited to specific analytes, and quantitative analyses (e.g.…”
Section: Introductionmentioning
confidence: 99%
“…The role for high glucose in signaling is an extraordinarily complicated problem to solve, because it must take into consideration that d-glucose is rapidly taken up, a high concentration of sucrose occurs in apoplastic transport and must encounter localized invertases within their cell walls, cells may have desensitization mechanisms operating as a function of concentration and exposure time, and there are no physiological assays for d-glucose responses with response times in minutes to assess this (current sugar sensitivity assays are in days using organ growth acclimated in applied glucose concentrations). In vivo extracellular sensors for d-glucose (Deuschle et al, 2005) are required to answer unequivocally this question, but these are not yet available. However, indirect measurements have been applied to address this problem, and estimates of apoplastic levels of d-glucose at 150 mM (3%) or higher have been suggested (McLaughlin and Boyer, 2004;Makela et al, 2005).…”
Section: Discussionmentioning
confidence: 99%
“…GFP mutants with shifted wavelengths of excitation or emission have been used as FRET donors and acceptors (Miyawaki, 2003;Miyawaki et al, 1997). GFP-based sensors can be expressed in situ by gene transfer techniques, and have therefore been applied, in particular, for cellular imaging of physiological activity in complex systems, a field extensively reviewed (Ehrhardt, 2003;Deuschle et al, 2005;Miyawaki, 2003;Fehr et al, 2005;Chudakov et al, 2005). As an example, calmodulin which undergoes a conformational change upon binding to calcium, was used to induce FRET between a donor and acceptor variant of attached GFP.…”
Section: Methods For Labeling Bindersmentioning
confidence: 99%