Genetically encoded RNA nanodevices for cellular imaging and regulation

Yu, Qikun; You, Mingxu

doi:10.1039/d0nr08301a

Cited by 14 publications

(18 citation statements)

References 184 publications

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“…A spatio-temporally resolved analysis indicated that sequestration of endogenous beta-actin mRNA attenuated cell motility through the regulation of focal-adhesion dynamics. There is also a broad field of genetically encoded photo-responsive RNA nanodevices [ 89 ]. From mere light-regulated RNA switches based on the specific recognition of RNA aptamers towards a particular photo-induced isomerization state of a chromophore to actual photo-responsive RNA nanodevices engineered to specifically recognize a bacterial light-oxygen-voltage photoreceptor and sterically inhibit gene expression or generate reactive oxygen species (ROS) upon light irradiation and lead to cell structure damage and photodynamic therapy, these reversible and irreversible photo-regulated RNA nanodevices can be potentially used to precisely regulate cell functions.…”

Section: The Optogenetic Toolboxmentioning

confidence: 99%

“…Moreover, the advanced optogenetic based biosensing and related biomaterials could fuel applications in transversal, emerging domains: Design and engineer synthetic genetically encoded functional nucleic acids FNAs nanostructures and nanodevices [ 89 ], extending the traditional biological roles of nucleic acids as catalytic enzymes, intracellular regulatory molecules and carriers of genetic information towards directing the assembly and functionality of materials at the nanoscale. Versatile FNAs-based, light-controlled nanodevices are expected to be broadly used in the near future to probe and program cells and other biological systems (e.g., regulating and compartmentalizing cellular gene expression, imaging, logic operation).…”

Section: Wide Biosensing Relevancementioning

confidence: 99%

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Advanced Optogenetic-Based Biosensing and Related Biomaterials

et al. 2021

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The ability to stimulate mammalian cells with light, brought along by optogenetic control, has significantly broadened our understanding of electrically excitable tissues. Backed by advanced (bio)materials, it has recently paved the way towards novel biosensing concepts supporting bio-analytics applications transversal to the main biomedical stream. The advancements concerning enabling biomaterials and related novel biosensing concepts involving optogenetics are reviewed with particular focus on the use of engineered cells for cell-based sensing platforms and the available toolbox (from mere actuators and reporters to novel multifunctional opto-chemogenetic tools) for optogenetic-enabled real-time cellular diagnostics and biosensor development. The key advantages of these modified cell-based biosensors concern both significantly faster (minutes instead of hours) and higher sensitivity detection of low concentrations of bioactive/toxic analytes (below the threshold concentrations in classical cellular sensors) as well as improved standardization as warranted by unified analytic platforms. These novel multimodal functional electro-optical label-free assays are reviewed among the key elements for optogenetic-based biosensing standardization. This focused review is a potential guide for materials researchers interested in biosensing based on light-responsive biomaterials and related analytic tools.

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Section: The Optogenetic Toolboxmentioning

confidence: 99%

Section: Wide Biosensing Relevancementioning

confidence: 99%

Advanced Optogenetic-Based Biosensing and Related Biomaterials

et al. 2021

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“…As a result, different RNAs, proteins, and small-molecule analytes can be detected and imaged by these RNA-based fluorescent sensors. 11-14 On the other hand, RNA-based bioluminescent sensors remain largely underdeveloped. RNA-based genetic regulators can induce bioluminescence signals by controlling the cellular expression of luciferase reporters.…”

Section: Introductionmentioning

confidence: 99%

“…RNA-based genetic regulators can induce bioluminescence signals by controlling the cellular expression of luciferase reporters. 2,11,15 However, these RNA nanodevices can rarely be used as biosensors for the real-time detection or monitoring of cellular target analytes.…”

Section: Introductionmentioning

confidence: 99%

“…22,23 We and others have previously demonstrated that these FRs can be engineered into modular fluorescent sensors for the sensitive imaging of various cellular targets. 11-14,24-29 We speculate that once our RNA-based BRET platform was established, these existing fluorescent RNA sensors can be readily converted to generate target-specific bioluminescence signals.…”

Section: Introductionmentioning

confidence: 99%

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Genetically Encoded RNA-based Bioluminescence Resonance Energy Transfer (BRET) Sensors

Mudiyanselage

et al. 2022

Preprint

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RNA-based nanostructures and molecular devices have become popular for developing biosensors and genetic regulators. These programmable RNA nanodevices can be genetically encoded and modularly engineered to detect various cellular targets and then induce output signals, most often a fluorescence readout. Although powerful, the high reliance of fluorescence on the external excitation light raises concerns on its high background, photobleaching, and phototoxicity. Bioluminescence signals can be an ideal complementary readout for these genetically encoded RNA nanodevices. However, RNA-based real-time bioluminescent reporters have been rarely developed. In this study, we reported the first type of genetically encoded RNA-based bioluminescence resonance energy transfer (BRET) sensors that can be used for real-time target detection in living cells. By coupling a luciferase bioluminescence donor with a fluorogenic RNA-based acceptor, our BRET system can be modularly designed to image and detect various cellular analytes. We expect this novel RNA-based bioluminescent system can be potentially used broadly in bioanalysis and nanomedicine for engineering biosensors, characterizing cellular RNA-protein interactions, as well as high-throughput screening or in vivo imaging.

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